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Filming ProtocolFree in-browser calculator

Zebrafish Swimming Video Checklist.

Generate a printable filming protocol with camera positions, swimming assay types, apparatus, acclimation notes, and standardized file naming for zebrafish locomotion studies or ConductVision upload.

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PrivateData stays in your browser
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Validated2026-04-09
CitableMethods and citation included

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Load example zebrafish swimming video checklist data to see the full workflow

Session Info

Swimming Types

Camera Positions

Apparatus

Filming Protocol (0 clips)

Subject ID / genotype is empty.
No locomotion types selected. Select at least spontaneous swimming.
No camera positions selected.
No apparatus selected.

File naming: _larval_5dpf_2026-06-09_[LOCO]_[CAMERA]_[APPARATUS]_trial[N]

Select swimming types, camera positions, and apparatus to generate your protocol.

Analyze your zebrafish swimming videos with ConductVision

Upload your standardized clips for AI-powered locomotion analysis, swim speed quantification, and behavioral scoring.

Go to ConductVision

Filming protocol follows established zebrafish behavioral methodologies (DanioVision, ZebraBox, EthoVision XT). Upload completed videos to ConductVision for AI locomotion analysis.

How It Works

Select the developmental stage (3/5/7 dpf larva, juvenile, adult), swimming assay types (spontaneous swimming, light/dark VMR, acoustic startle, prey capture, thigmotaxis, seizure locomotion), camera positions (dorsal, lateral, ventral, stereo), and apparatus (96/24/6-well plates, petri dish, swim tunnel, novel tank). Optionally enable high-speed video, acclimation protocols, and IR backlight notes. The tool generates a numbered shot list with zebrafish-specific instructions and duration. Incompatible combinations (e.g. lateral camera with 96-well plate) are automatically filtered.

Filming Best Practices

Temperature is critical: maintain 28.5°C ± 0.5°C throughout recording. Use fresh E3 embryo medium for larvae, not system water. For VMR (visual motor response): standard protocol alternates 10-min light and 10-min dark phases. Use IR backlight for tracking during dark phases without disturbing fish behavior. Degas medium before recording to prevent bubbles that disrupt tracking. Mark plate orientation for consistent light angles across experiments. Frame rate: 30 fps for standard tracking, 1000+ fps for C-start escape kinematic analysis. For novel tank tests, start recording immediately after transfer — the novelty IS the stimulus.

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Method

Filming protocol follows established zebrafish behavioral methodologies including DanioVision (Noldus), ZebraBox (ViewPoint), and EthoVision XT tracking conventions. Camera positions and apparatus settings follow published best practices for larval and adult zebrafish behavioral assays. Acclimation protocols include temperature equilibration, E3 medium preparation, and IR dark-phase recording setup. All computation runs locally in your browser — no data is uploaded.

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Validated

Last validated 2026-04-09. Calculations are designed for planning and documentation support; verify procurement decisions against manufacturer specifications or institutional SOPs.

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How to cite

How to Cite

ConductScience Team (2026). Zebrafish Swimming Video Checklist [Web application]. ConductScience. https://conductscience.com/tools/zebrafish-swimming-video-checklist

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Next steps

Protocol

ConductVision Locomotion Analysis

Reference protocol for the next planning step.

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Zebrafish Novel Tank Analyzer

Reference protocol for the next planning step.

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Open Field Test Analyzer

Reference protocol for the next planning step.

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