MicroED datasets from native nanocrystalline granulovirus polyhedrin recorded with a Falcon III camera

<p>The&nbsp;<em>Cydia pomonella </em>crystalline&nbsp;occlusion bodies were provided by&nbsp;Dominik Oberth&uuml;r (CFEL).</p> <p><strong>Data collection</strong></p> <p>Diffraction data sets were collected for 31 crystals (gv_09 does not exist), following&nbsp;this scheme:</p> <ul> <li>Images were collected on a 300 kV Krios system (Thermo Fisher Scientific), using a Falcon III camera. The condenser system was used in nanoprobe mode, making a parallel beam of size 0.6 &micro;m. A camera length of 3.1 m was chosen, providing 2.1 &Aring;&nbsp;resolution at the edge of the detector</li> <li>The camera was used in linear mode, and with dose protector disabled, using 1s integration time. Data was unbinned</li> <li>The gain was determined to be ~10 counts/pe (gain for single frame is 390, for 39 frames averaged the gain is 10)</li> <li>The stage had a tilt speed of 0.5 deg/s</li> <li>The dose rate was 0.3 el/&Aring;²/s</li> <li>For each crystal ~10 frames were collected before severe radiation damage</li> <li>The samples&nbsp;charged slightly, leading to defocused and astigmatic spots in some cases</li> </ul> <p><strong>Data processing</strong></p> <p>A script for processing data sets from 22 crystals with&nbsp;DIALS version 1.14.4 and&nbsp;CCP4 7.0.073 is provided. This script will need adapting to work with newer versions of the software.</p>