Phasor Dataset generated at NEST (Scuola Normale Superiore)

<p>The folder contains sub-folders used in the work "Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks" by M.Bernardi and F.Cardarelli. The data served for specific examples on Phasor-FLIM customary analysis within the framework of the Python Notebook ran in Google Colab, extensively documented and available at: <a href="https://github.com/Mariochem92/PhasorIdentifier">https://github.com/Mariochem92/PhasorIdentifier</a></p><p>&nbsp;</p><p>&nbsp;</p><p><strong>Irinotecan and its metabolite SN-38 in solution at different pH</strong></p><p><strong>File format:&nbsp;</strong>.R64</p><p><strong>FLIM Acquisition:</strong> DFD-FLIM&nbsp;</p><p><strong>Laser repetition frequency:</strong> 80 MHz</p><p><strong>Instrumentation:&nbsp;</strong>Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)</p><p>Can be used to test any functionality in the analysis of evolutions within the phasor plot. Specially designed for pH analysis within the pH range of 2 to 12.<br><strong>Reference:&nbsp;</strong>Bernardi et al., A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks, 2023</p><p>&nbsp;</p><p><strong>Encapsulated irinotecan in commercial liposomal nanoformulation Onivyde&nbsp;</strong></p><p><strong>File format:&nbsp;</strong>.R64</p><p><strong>FLIM Acquisition:</strong> DFD-FLIM&nbsp;</p><p><strong>Laser repetition frequency:</strong> 80 MHz</p><p><strong>Instrumentation:&nbsp;</strong>Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)</p><p>Can be used to test any functionality in the analysis of the supramolecular organization.&nbsp;</p><p><strong>Reference:&nbsp;</strong>Bernardi et al., Fluorescence Lifetime Nanoscopy of Liposomal Irinotecan Onivyde: From Manufacturing to Intracellular Processing, ACS Appl. Bio Mater., 2023</p><p>&nbsp;</p><p><strong>INS1-E cells upon cytokine treatment</strong></p><p><strong>File format:&nbsp;</strong>.R64</p><p><strong>FLIM Acquisition:</strong> DFD-FLIM&nbsp;</p><p><strong>Laser repetition frequency:</strong> 80 MHz</p><p><strong>Instrumentation:&nbsp;</strong>Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)</p><p>INS1E control and INS1E cytokines, can be used to test any functionality in the analysis of the supramolecular organization.&nbsp;<br><strong>Reference:&nbsp;</strong>Pugliese et al, Unveiling nanoscale optical signatures of cytokine-induced β-cell dysfunction, Sci Rep, 2023</p><p>&nbsp;</p><p><strong>Encapsulated doxorubicin&nbsp;</strong></p><p><strong>File format:&nbsp;</strong>.ref</p><p><strong>FLIM Acquisition:</strong> TD-FLIM&nbsp;</p><p><strong>Laser repetition frequency:</strong> 40 MHz</p><p><strong>Instrumentation:&nbsp;</strong>Leica TCS SP5 confocal microscope</p><p>Can be used to test any functionality in the analysis of the supramolecular organization and signal evolution. Specially designed for storage analysis at temperature 4°C, 25°C and 37°C.<br><strong>Reference:&nbsp;</strong>Carretta et al, Monitoring drug stability by label-free fluorescence lifetime imaging: a case study on liposomal doxorubicin. ,J. Phys. : Conf Ser, 2023</p><p>&nbsp;</p><p>&nbsp;</p>