Fig. 1 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 1. (a) Purification of peptides from Satureja khuzistanica leaves. Two hundred fifty μL aliquot of lyophilized extract subjected to reverse phase-HPLC column by using a gradient from 5 to 65% (v/v) solution B (0.098% TFA in acetonitrile) combined with solution A (0.1% TFA in water) during 85 min. The absorbance was monitored at wavelength 220 nm. Totally, 15 peaks were collected. (b) The purity peak S2 was evaluated using the same column and method. (c) Tricine-SDS-PAGE profile of (1) protein standards from 2 kDa to 250 kDa (2) peptide extract passed through an ultra membrane with a 10-kDa cutoff, (3) The peak S2 purified from RP-HPLC column.