OTUs Table and fastq sequences from environmental DNA applied to trematode communities

<p>This OTUs table and fastq sequences underlie the main results of the study "Make visible the invisible: Optimized development of an environmental DNA metabarcoding tool for the characterization of trematode parasitic communities".</p> <p>In this study, our aim was to develop an optimized eDNA-based metabarcoding approach to detect trematodes and characterize their communities, most of which associated to aquatic environments. We thus assessed the ability of our eDNA-based metabarcoding approach to reconstruct trematode communities compared to a classical trematode monitoring method over four freshwater aquatic ecosystems. </p> <p>We focused on 4 natural sites from Occitanie Region (Southern France) that differ in terms of habitats, and in which the trematode communities were previously at least partially characterized. At each of these sites, we sampled the water-sediment interface from which the eDNA was extracted and sequenced with a MiSeq amplicons sequencing approach.  <br> Over the four natural ecosystems screened in nature, 33 OTUs were generated from the eDNA-based approach, from which 11 trematode species were identified. In comparison, we identified five trematode species using the classical monitoring method, three of which were also detected by the eDNA-based approach.</p>