3D+time nuclei tracking dataset of diSPIM lightsheet fluorescence microscopy time series of C. elegans embryos

<p>The dataset consists of 3 diSPIM microscopy time series of <em>C. elegans</em> embryos, fully tracked.</p> <ul> <li>3 raw time-series and the corresponding tracks/lineage trees</li> <li>temporal resolution: 1min</li> <li>temporal extent: 350-400 frames, tracked for at least 330 frames</li> <li>spatial resolution (zyx): 0.1625 x 0.1625 x 0.1625&mu;m</li> <li>spatial extent (zyx): 250 x 250 x 400px (average)</li> <li>Microscope: dual-view ASI diSPIM (fused and deconvolved using the MIPAV GenerateFusion plugin)</li> </ul> <p>The original raw data and annotations were part of the following publication (please also cite this if you use the dataset):</p> <p><em>&nbsp;&nbsp; </em>Moyle, M.W., Barnes, K.M., Kuchroo, M. <em>et al.</em> Structural and developmental principles of neuropil assembly in <em>C. elegans</em>. <em>Nature</em> 591<strong>, </strong>99&ndash;104 (2021). <a href="https://doi.org/10.1038/s41586-020-03169-5">https://doi.org/10.1038/s41586-020-03169-5</a></p> <p>Additionally the data was extended and slightly curated further by Peter Hirsch (MDC) and used for the development of a new tracking method in the following publication:</p> <p><em>&nbsp;&nbsp; Hirsch, P., Malin-Mayor, C., Santella, A., Preibisch, S., Kainmueller, D., Funke, J. Tracking by weakly-supervised learning and graph optimization for whole-embryo C. elegans lineages. MICCAI 2022</em></p> <p>For questions please contact Peter Hirsch (<a href="mailto:peter.hirsch@mdc-berlin.de">peterhirsch@posteo.de</a>).</p>