Data supplement to Manuscript "A 5-Gene Signature (PROGRESS-STYCK) predicts ICU Admission and Death in Community Acquired Pneumonia"

<p>Supplementary RNAseq data for correlation analysis with HT12v4 Chip data</p> <p>This refers to the publication</p> <p><strong>A 5-Gene&nbsp;</strong><strong>Signature (PROGRESS-STYCK) predicts ICU Admission and Death in Community Acquired Pneumonia</strong></p> <p>Holger Kirsten¹*, Sebastian Weis^2,3,4*, Peter Ahnert¹, Martin Witzenrath^5,6, Brendon P. Scicluna^7,8, Knut Krohn⁹, Friedmann Horn¹⁰Michael Rade¹⁰, Catharina Bertram¹⁰, Kristin Reiche¹⁰, Dennis L&ouml;ffler¹⁰, Conny Blumert¹⁰, Kai Sohn¹¹, Stefan Jenner¹¹,, Kai Sohn¹¹, Geraldine Nouailles⁵, Michael Kiehntopf^12,13, Petra Creutz⁵, Markus Loeffler¹, Norbert Suttorp^5,6,PROGRESS Study Group, Markus Scholz¹⁺, Michael Bauer²⁺</p> <p>&nbsp;</p> <p>¹ Institute for Medical Informatics, Statistics and Epidemiology (IMISE), Leipzig University, Leipzig, Germany,</p> <p>² Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital,</p> <p>Friedrich-Schiller-University Jena, Germany</p> <p>³Institute for Infectious Disease and Infection Control, Jena University Hospital, Friedrich-Schiller-University Jena, Jena, Germany</p> <p>⁴Leibniz Institute for Leibniz Institute for Natural Product Research and Infection Biology</p> <p>Hans Kn&ouml;ll Institute, Jena</p> <p>⁵Charit&eacute; - Universit&auml;tsmedizin Berlin, corporate member of Freie Universit&auml;t Berlin and Humboldt-Universit&auml;t zu Berlin, Department of Infectious Diseases and Respiratory Medicine, Berlin, Germany.</p> <p>⁶ German Center for Lung Research (DZL), Partner Site Charit&eacute;, Berlin, Germany.</p> <p>⁷ Centre for Molecular Medicine and Biobanking, University of Malta, Malta</p> <p>⁸ Department of Applied Biomedical Science, Faculty of Health Sciences, Mater Dei hospital, University of Malta, Malta</p> <p>⁹ Core Unit DNA Technologies, Medical Faculty, Leipzig University, Leipzig, Germany.</p> <p>¹⁰ Department of Diagnostics, Institute for Cell Therapy and Immunology, Leipzig, Germany</p> <p>¹¹ Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany</p> <p>¹²Department of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Friedrich-Schiller-University Jena, Jena, Germany.</p> <p>¹³Integrated Biobank Jena (IBBJ), Jena University Hospital, Friedrich-Schiller-University Jena, Jena, Germany.</p> <p>*+ these authors contributed equally to the work.</p> <p>&nbsp;</p> <p>For sequencing-based expression quantification, libraries were prepared using globin-mRNA depleted RNA and sequencing was performed with HiSeq2500 sequencing (Illumina, San Diego, CA, USA), with an average sequencing depth of 100 million clusters per sample and 2x100b paired-end reads. Data analysis included demultiplexing, trimming, filtering, removal of low-quality bases, quantification at gene-level, and quality-assessment. Data of measured genes and individuals can be found in file&nbsp;<strong>s816_1_expression_levels_ngs.txt</strong>.&nbsp;&nbsp;</p> <p>For array-based expression quantification, purified RNA was hybridized to Illumina HT-12v4 Expression-BeadChips (Illumina, San Diego, CA, USA). Low-quality samples were removed, and data was log2-transformed, quantile-normalized, batch-corrected and filtered for minimum expression levels, resulting in 26,601 transcripts representing 16,329 unique genes.Data of measured genes and individuals can be found in&nbsp; <strong>s816_1_expression_levels_array.txt</strong></p> <p>Data showing corresponding genes between NGS gene IDs and Array probe IDs can be found in <strong>s816_1_assignment_genes_ngs_array.txt</strong><br> &nbsp;</p>