RepliChrom: Interpretable machine learning predicts cancer-associated enhancer-promoter interactions using DNA replication timing

<p>This dataset accompanies the study "RepliChrom: Interpretable machine learning predicts cancer-associated enhancer-promoter interactions using DNA replication timing". The study introduces RepliChrom, a computational framework designed to predict enhancer&ndash;promoter interactions (EPIs) by leveraging multi-scale replication timing (RT) signals. This approach addresses the fundamental challenge of distinguishing gene targets regulated by distal enhancers from those activated by proximal transcriptional activity-a key problem in understanding the causal basis of complex diseases.</p> <p>Despite recent advances in high-throughput technologies such as Hi-C, ChIA-PET, and Hi-TrAC that allow genome-wide reconstruction of 3D chromatin architecture, the role of DNA replication timing in mediating these spatial interactions remains underexplored. RepliChrom fills this gap by using cell-type-specific RT profiles as predictive features for chromatin interaction inference.</p> <p>To support model development, training, and evaluation, we provide a comprehensive multi-omics dataset covering six human cell lines (K562, GM12878, HeLaS3, IMR90, NHEK, and HUVEC ), encompassing:</p> <p>Hi-C datasets: Processed chromatin interaction loops used to define positive and negative enhancer&ndash;promoter interaction pairs.</p> <p>ChIA-PET datasets: Interaction data anchored around transcription factor binding, including POLR2A and CTCF, used for model validation across different interaction types.</p> <p>Hi-TrAC datasets: Targeted chromatin accessibility-derived interaction data, offering complementary validation of the model on alternate platforms.</p> <p>Replication Timing (RT) data: Processed RT signal profiles for each cell line, used to extract multi-scale temporal features as inputs for RepliChrom.</p> <p>These datasets enable reproducibility of the model training process and serve as benchmark resources for future research into DNA replication&ndash;mediated regulation of 3D genome architecture.</p> <p><strong>Included Files</strong></p> <p>Hi-C_datasets.zip (2.23 MB): Processed Hi-C interaction pairs for six cell types.</p> <p>ChIA-PET_datasets.zip (818.18 KB): CTCF and POLR2A ChIA-PET interactions across multiple lines.</p> <p>Hi-TrAC_datasets.zip (196 bytes): Hi-TrAC-based chromatin interaction training datasets across multiple lines.</p> <p>Cellline_RT_data.zip (86.17 MB): Replication timing signal data across multiple human cell types for multi-scale replication timing feature extraction.</p> <p><strong>Usage</strong><br>All datasets are intended for academic, non-commercial use. The provided files can be directly used to reproduce the training and evaluation of RepliChrom, and may also support broader applications in enhancer&ndash;promoter modeling, replication-timing analysis, and 3D genomics studies. For detailed usage instructions and code implementation, please refer to the GitHub repository: https://github.com/DaoFuying/RepliChrom</p>