Fig. 4 in Zavreliella shidai Han & Liu & Luo & Tang 2021, sp. n.

Fig. 4. Inmunodetection of P-gp in P. acuta snails using the anti-P-gp C219 antibody. (A) Expression of ~170 kDa and 220-240 kDa bands of P-gp was analysed on collection day and following a depuration period of 7 days by Western blot. The relative level of P-gp using α-tubulin as the internal control is represented in the bar graph. Data are given in intensity units and represent a mean ± SD from three separate experiments. (B) Calibration curve for dot blot analysis using C219 antibody. Upper panel: labeling of monoclonal antibody to increasing amounts of total protein from P. acuta homogenates (0.22, 0.44, 0.88, 1.75, 3.50 and 7.00 µg.µl-1 of protein). Bottom panel: Standard curve of intensity of the label for each dot. Data represent a mean ± SD from six separate experiments. The X-axis (µg.µl-1 of proteins) is on a log scale.