Self-propagating wave drives noncanonical antidurotaxis of skull bones in vivo

<p>Cellular motion is a key feature of tissue morphogenesis and is often driven by migration. However, migration, need not explain cell motion in contexts where there is little free space or no obvious substrate such as those found during organogenesis of mesenchymal organs including the embryonic skull. Through <em>ex vivo</em> imaging, biophysical modeling, and perturbation experiments, we find that mechanical feedback between cell fate and stiffness drives bone expansion and controls bone size <em>in vivo</em>. This mechanical feedback system is sufficient to propagate a wave of differentiation that establishes a collagen gradient which we find sufficient to describe patterns of osteoblast motion. Our work provides a mechanism for coordinated motion that may not rely upon cell migration but on emergent properties of the mesenchymal collective. Identification of such alternative mechanisms of mechanochemical coupling between differentiation and morphogenesis will help in understanding how directed cellular motility arises in complex environments with inhomogeneous material properties.</p>