Putative Looping Factor ZNF143/ZFP143 is an Essential Transcriptional Regulator with No Looping Function

<h1>Overview</h1> <p>This repository contains all the raw fastSPT, FRAP, absolute abundance quantification data, and DESeq2 outputs associated with &ldquo;Putative Looping Factor ZNF143/ZFP143 is an Essential Transcriptional Regulator with No Looping Function&rdquo;.&nbsp;</p> <h1>Cell lines, conditions, and experiments</h1> <p>This repository has data for the following experiments, cell lines, and conditions.&nbsp;</p> <p><strong>fastSPT</strong> tracks and <strong>FRAP</strong> quantification for:</p> <ul> <li>Histone H2B</li> <li>Halo-NLS</li> <li>CTCF C87</li> <li>Clone D untreated</li> <li>Clone D ∆ZFP143 (2-4h)</li> <li>Clone A ZFP143 untreated</li> <li>Clone A ZFP143 ∆CTCF (2-4h)</li> <li>Clone B ZFP143 untreated</li> <li>Clone B ZFP143 ∆CTCF (2-4h)</li> </ul> <p><strong>FRAP</strong> quantification for:</p> <ul> <li>Clone A CTCF untreated</li> <li>Clone A CTCF ∆ZFP143 (2-4h)</li> <li>Clone B CTCF untreated</li> <li>Clone B CTCF ∆ZFP143 (2-4h)</li> <li>WT mESC pDNN13 transfection</li> <li>WT mESC pDNN14 transfection</li> <li>WT mESC pDNN15 transfection</li> </ul> <p><strong>DESeq2</strong> outputs for:</p> <ul> <li>Clone A/B ∆ZFP143 vs untreated PRO-seq</li> <li>Clone A/B ∆CTCF vs untreated PRO-seq</li> <li>Clone A/B ∆ZFP143/∆CTCF vs untreated PRO-seq</li> <li>Clone D ∆ZFP143 vs untreated PRO-seq</li> </ul> <p><strong>Other files</strong>:</p> <ul> <li>ZFP143 abundance quantification</li> <li>The parameters used for tracking (<em>tracking_config.toml</em>)</li> <li>The parameters used to get nuclear masks for fastSPT data (<em>masking_params.csv</em>)</li> </ul>