Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

<p>We provide here the underlying data of the publication &quot;Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase&quot;. Please find the abstract below.</p> <p>Advanced biotransformation processes typically involve the upstream processing part performed continuously and&nbsp;interlinked tightly with the product isolation. Key in their development is a catalyst that is highly active,&nbsp;operationally robust, conveniently produced and recyclable. A promising strategy to obtain such catalyst is to&nbsp;encapsulate enzymes as permeabilized whole cells in porous polymer materials. Here, we show immobilization of&nbsp;the sucrose phosphorylase from Bifidobacterium adolescentis (P134Q-variant) by encapsulating the corresponding&nbsp;E. coli cells into polyacrylamide. Applying the solid catalyst, we demonstrate continuous production of the&nbsp;commercial extremolyte 2-&alpha;-D-glucosyl-glycerol (2-GG) from sucrose and glycerol. The solid catalyst exhibited similar activity (&ge;70%) as the cell free extract (~800 U g-1&nbsp;cell wet weight) and showed excellent in-operando&nbsp;stability (40 &deg;C) over 6 weeks in a packed-bed reactor. Systematic study of immobilization parameters related to&nbsp;catalyst activity led to the identification of cell loading and catalyst particle size as important factors of process&nbsp;optimization. Using glycerol in excess (1.8 M), we analyzed sucrose conversion dependent on space velocity (0.075 &ndash; 0.750 h-1) and revealed conditions for full conversion of up to 900 mM sucrose. The maximum 2-GG space-time yield reached was 45 g L-1 h-1 for a product concentration of 120 g L-1. Collectively, our study&nbsp;establishes a step-economic route towards a practical whole cell-derived solid catalyst of sucrose phosphorylase, enabling continuous production of glucosides from sucrose. This strengthens the current biomanufacturing of 2-GG, but also has significant replication potential for other sucrose-derived glucosides, promoting their industrial scale production using sucrose phosphorylase.&nbsp;&nbsp;</p>