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RNA-seq data for SARS-CoV-2 infected cells

<p>We performed our sequencing and analysis in CapitalBio Technology (Beijing, China). A549 cells were infected with SARS-CoV-2, panH1N1 and H7N9&nbsp;at a MOI of 0.01&nbsp;for 60&nbsp;hr. Total&nbsp;RNAs from control and virus infected cells were extracted&nbsp;using the TRIzol reagent according to the manufacturer&rsquo;s instructions (Invitrogen, USA). The&nbsp;genomic DNA was removed using DNase I (Takara, Japan). RNA samples were assessed for their quality using the RNA 6000&nbsp;pico kit (Agilent, USA) and quantified using the ND-2000 (NanoDrop Technologies).&nbsp;Only the high quality RNA was selected to construct the sequencing library with the Illumina&nbsp;TruSeq Stranded mRNA Library Preparation kit (Illumina, USA).&nbsp;The NEBNext Multiplex Small RNA Library Prep Set for Illumina&nbsp;(NEB, USA) was used for RNA library preparation.</p>

ShareScore

24/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
16
Reuse readiness
0
Engagement
0