RNA sequencing of nuclease dead PET127 conditions

<p><em>PET127, </em>is thought to be a mitochondrial RNA exonuclease and loss of <em>PET127</em> is thought to cause accumulation of untrimmed RNA in the mitochondria. Here we show RNA sequencing of <em>Saccharomyces cerevisiae</em> in four <em>PET127</em> conditions: <em>PET127</em> wild-type, <em>pet127∆, pet127-nd </em>(nuclease dead)/<em>pet127∆, </em>and <i>pet127-nd</i>/<i>PET127. </i>The resulting sequences show that regions of mitochondrial RNA accumulate in the <i>pet127</i><em>∆</em> background, and the RNA sequencing profile of <em>PET127</em> containing disruptive mutations in the conserved exonuclease active site mimics that of <em>pet127∆</em>. </p>