Figure 3 from: Nilsson R, Tedersoo L, Abarenkov K, Ryberg M, Kristiansson E, Hartmann M, Schoch C, Nylander J, Bergsten J, Porter T, Jumpponen A, Vaishampayan P, Ovaskainen O, Hallenberg N, Bengtsson-Palme J, Eriksson K, Larsson K, Larsson E, Kõljalg U (2012) Five simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequences. MycoKeys 4: 37-63. https://doi.org/10.3897/mycokeys.4.3606

Figure 3 - "Strand=Plus/Minus" indicates that the query and reference sequence come in opposing read directions. Another hint comes from the observation that the alignment starts at the first base (1) in the query sequence and progresses upwards to base 60 in the first alignment line; however, for the reference sequence, the alignment starts at base 635 and progresses downwards to base 578.