Brucker Maldi-TOF custom database for differentiating Enterococcus faecium clades A1/A2 from B

<p>This custom database has been constructed using MBT explorer software (Bruker &reg;) and the Maldi Biotyper (Bruker). Protein extraction were performed according to the MSP Creation protocol (V1.1, Bruker &reg;) and mass spectra were obtained following the Maldi Biotyper protocol (V.2.4, Bruker &reg;). The resulting spectra were carefully inspected using flexAnalysis software (V3.4, Bruker &reg;). After elimination of spectra with mass peak deviation &gt; 0.05%, outlier peaks or flatline, the remaining spectra were combined to generate a single mass spectrum for each strain onto the MALDI Biotyper software (V4.1, Bruker &reg;), with default parameters. The mass spectra were used to generate this new database, available on the MBT Compass RUO software (RevC Version, Bruker &reg;).&nbsp;&nbsp;</p> <p>The individual spectra are available as well as the MSP (Mass Spectrum Profile) for each strains.</p>