Fig. 2. A in Psilostachyins as trypanocidal compounds: Bioguided fractionation of Ambrosia tenuifolia chemically modified extract

Fig. 2. A) Schematic representation of bioguided separation of CH2Cl2-m-CPBA modified extract of Ambrosia tenuifolia to isolate and identify the main SLs responsible for the trypanocidal activity. B) Percentage of parasite inhibition after treatment with the pools (I-VI) and benznidazole at 5 μg/mL. Asterisks indicate significant differences between the indicated groups and the control, benznidazole (****, p <0.001), and ns indicates no significant differences. C) Percentage of parasite inhibition after treatment at different concentrations (1, 5 and 10 μg/mL) with the pure compounds obtained after chemical extract derivatization. At 1 and 5 μg/mL, only the indicated groups did not exhibit significant differences compared to the respective concentration of benznidazole (ns). At 10 μg/mL, the indicated group showed significant differences compared to benznidazole at the same concentration (****, p <0.001). The molar concentrations equivalent to 1 μg/mL of each compound are 3.8 μM (Psi C and benznidazol) and 3.6 μM (Psi A, paulitin, isopaulitin, 2 and 3), for more details see figure S8.