Fig. 6 in Diversity of sesquiterpenoids from Stellera chamaejasme with neuroprotective effects

Fig. 6. Neuroprotective effects of compounds on SNP-induced neurotoxicity in PC-12 cells. (A) Effects of compounds 1–20 on SNP-induced injury in PC12 cells at a concentration of 10 μM. Cells were pretreated with or without samples (1–20 and the positive control Eda) at for 2 h and then incubated with or without SNP (600 μM) for another 24 h. Cell viability was evaluated by the MTT method (n = 3). *p <0.05, **p <0.01, and ***p <0.001 compared with SNP alone group. (B) Effects of active compounds 5, 10, 11, 15, 16, and 18 on SNP-induced injury in PC12 cells at the concentrations of 2.5, 5, and 10 μM. The treatment and result analysis of the experiment were described as above. (C) Effects of the most active compound 11 on ROS production in PC-12 cells. After the pretreatment of the cells with or without 11 (10 μM) for 2 h, the cells were then exposed to SNP (600 μM) for another 24 h and followed by incubation with DCFH-DA (20 μM) for 30 min. Photography was taken with a fluorescence microscope. (D) Compound 11 could restore the MMP of PC-12 cells induced by SNP. Cells were pretreated with or without 11 (10 μM) for 2 h and then exposed to SNP (600 μM) for another 24 h. The MMP was measured by the JC-1 dye method. (E) Effects of 11 on level of Ho-1 mRNA in PC-12 cells. Cells were pretreated with or without indicated concentrations of 11 for 2 h and then incubated with or without SNP (600 μM) for another 24 h. After that, the targeted mRNA (Ho-1) was detected by quantitative realtime RT-PCR. The mRNA level was normalized to Gapdh and expressed as fold changes of the control (n = 3). *p <0.05 and ***p <0.001 versus the control (CT) group.