Fig. 3 in Oxylipin biosynthesis in spikemoss Selaginella moellendorffii: Identification of allene oxide synthase (CYP74L2) and hydroperoxide lyase (CYP74L1)

Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 with 13-HPOT (A), 13- HPOD (B), 9-HPOT (C), and 9-HPOD (D). Conditions of incubation, extraction, derivatisation and analysis are described in Materials and Methods. 1a, 1b (threo and erythro isomers of 12,13-dihydroxy- 9,15-octadecadienoic acid (the products of the α-ketol 1 reduction with NaBH4)); 2a, 2b (threo and erythro isomers of 12,13-dihydroxy-9-octadecenoic acid (the products of the α-ketol 2 reduction with NaBH4)); 3a, 3b (threo and erythro isomers of 9,10- dihydroxy-12,15-octadecadienoic acid (the products of the α-ketol 3 reduction with NaBH4)); 4a, 4b (threo and erythro isomers of 9,10-dihydroxy-12-octadecenoic acid (the products of the α-ketol 4 reduction with NaBH4)); 7, 9-hydroxynonanoic acid. The structural formulae of products are present at Fig. 2. 13-HOT, (9Z,11E,13S,15Z)-13-hydroxy-9,11,15-octadecatrienoic acid; 13-HOD, (9Z,11E,13S)-13-hydroxy- 9,11-octadecadienoic acid; 9-HOD, (9S,10E,12Z)-9- hydroxy-10,12-octadecadienoic acid.