Kinetic properties of mAMCase catalytic domain at various pH

<p>This directory contains all files required to analyze the mouse AMCase enzyme kinetics across pH 2.0 to 8.0&nbsp;presented in <strong>Figure 1</strong> and <strong>Supplemental Figures 1 and 2</strong>&nbsp;the manuscript D&iacute;az et al., <em>bioRxiv </em>(2023).</p> <p>Enzyme kinetics were performed using a Tecan Spark multimode microplate reader&nbsp;and data was analyzed using Graphpad Prism. Figures were compiled using Adobe Illustrator.</p> <p>&nbsp;</p> <p>Files included in this directory:</p> <p><strong>20210906 - mAMCase_CatD_6xHis (pmRED006)</strong></p> <p>- 20210907 - Purification contains FPLC chromatograms and corresponding SDS-PAGE gel stained with InstantBlue.</p> <p>- 202110XX _pHX_100nM contains four replicates performed at that specific pH using 100 nM mAMCase.</p> <p>- 384w_exp.toml contains a&nbsp;384-well plate layout of the experimental conditions. This file is compatible with <a href="https://wellmap.readthedocs.io/en/latest/index.html">wellmap</a>.</p> <p>- 384w_std.toml contains a 384-well plate layout of the 4MU standards. This file is compatible with <a href="https://wellmap.readthedocs.io/en/latest/index.html">wellmap</a>.</p> <p>&nbsp;</p> <p><strong>Figures</strong><br> - contains PDFs of V_max,&nbsp;<em>k</em>_cat,K_M, catalytic efficiency, and the initial rate for all pH points tested.&nbsp;</p> <p>&nbsp;</p> <p><strong>20210906 - mAMCase_CatD_6xHis (pmRED006) - Data.pzfx</strong></p> <p>- contains all data from&nbsp;<strong>20210906 - mAMCase_CatD_6xHis (pmRED006), </strong>including standard curves, initial time points, and initial rates curves.&nbsp;</p> <p>&nbsp;</p> <p><strong>20210906 - mAMCase_CatD_6xHis (pmRED006) - Summary.pzfx</strong></p> <p>- contains summary data of kinetic parameters V_max,&nbsp;<em>k</em>_cat,K_M, catalytic efficiency, and initial rates for all pH points tested.</p> <p>&nbsp;</p> <p>Contact:<br> Roberto Efra&iacute;n D&iacute;az, robertoefrain.diaz@ucsf.edu</p> <p>James Fraser, jfraser@fraserlab.com</p>