Fig. 1 in Characterization of the PRODUCTION of ANTHOCYANIN PIGMENT 1 Arabidopsis dominant mutant using DLEMMA dual isotope labeling approach

Fig. 1. Overview of the DLEMMA workflow employed in this study. (A) Three differently labeled forms of phenylalanine (Phe) were used as feeding precursors, i.e. unlabeled, Label I (Phe-13C), and Label II (Phe-13C2H) Phe. (B) The two Arabidopsis genotype groups, WT and pap1-D, were fed with three forms of labeling 6 6 5 precursors for 24h. (C) Following feeding, the six treatments were combined (i.e. combinations A to F), in which four combinations were designed for metabolite identification and another two for semi-quantitative differential metabolite analysis. The extracts were analyzed by high resolution LC-MS in both positive and negative ion modes. (D) The LC-MS raw data was preprocessed with the R package XCMS, and next the R package Miso was used to detect and extract all Phe-derived mass features. (E) Phe-derived metabolites were identified based on retention time, m/z, MS/MS spectra, and dual-labeling patterns obtained from LC-MS analysis. (F) A combined sample matrices and label-swap approach was used to semi-quantitatively compare phenylpropanoids content between the WT and pap1- D genotypes.