rMATS analysis of alternative splicing events in a mouse model of environmental liver disease

<p>rMATS (https://rnaseq-mats.sourceforge.io/)&nbsp;was used to identify differential alternative splicing events (ASEs) corresponding to all five major types of AS patterns, <em>i.e</em>., skipped exon (SE), mutually exclusive exons (MXE), alternative 3&rsquo; splice site (A3SS), alternative 5&rsquo; splice site (A5SS), and retained intron (RI), in the HFD-fed mouse livers exposed to Ar1260, PCB126, or Ar1260 + PCB126 co-exposure compared to vehicle control This dataset identifies differential ASEs corresponding to all five major types of AS patterns [<em>i.e.,</em> skipped exon (SE), mutually exclusive exons (MXE), alternative 3&rsquo; splice site (A3&rsquo;SS), alternative 5&rsquo; splice site (A5&rsquo;SS), and retained intron (RI)], between Ar1260, PCB126, and Ar1260 + PCB126-exposed samples and vehicle control. For each ASE, the estimation of the alternatively spliced region usage is defined as percent-spliced in (&psi; or PSI). Each comparison was made to identify differential ASEs with an associated change in exon usage (∆&psi;). Differential ASEs were detected with an FDR of &lt;0.05 and |∆&psi;| of&ge;5%. The difference in the proportion of the two isoforms of the transcript was expressed as the change in mean percentage spliced-inform included (mean ∆&psi;).</p>