Fig. 7 in Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and noncanonical strigolactones in Lotus japonicus

Fig. 7. Conversion of [13C]-18-OH-MeCLA to [13C]-5DS and [13C]-lotuslactone (LL) in the feeding experiment using L. japonicus roots. A) Conversion of [10–13C]-18-OH-MeCLA to [6′-13C]-5DS. LC-MS/MS analysis of [6′-13C]-5DS in root exudates after feeding [10–13C]-18-OHMeCLA. MRM chromatograms (left) and full-scan spectra of fragment ions (right). The MRM chromatograms of authentic 5DS (red: 331.15/217.00, blue: 331.15/97.00, green: 331.15/234.00, m/z in positive mode) and [6′-13C]-5DS (red: 332.15/218.00, blue: 332.15/97.00, green: 332.15/ 235.00, m/z in positive mode) are shown. B) Conversion of [10–13C]-18- HO-MeCLA to [6′-13C]-LL. LC-MS/MS analysis of [6′-13C]-LL in root exudates after feeding [10–13C]-18-OH-MeCLA. MRM chromatograms (left) and full-scan spectra of fragment ions (right). The MRM chromatograms of authentic LL (red: 373.00/276.00, blue: 373.00/244.00, green: 373.00/ 216.00, m/z in positive mode) and [6′-13C]-LL (red: 374.00/277.00, blue: 374.00/245.00, green: 374.00/216.00, m/z in positive mode) are shown. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)