Fig. 6 in Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and noncanonical strigolactones in Lotus japonicus

Fig. 6. Conversion of [13C]-CLA to [13C]-5DS and [13C]-18-OH-CLA in the feeding experiment using L. japonicus roots. A) Conversion of [1-13CH]- 3 CLA to [8-13CH]-5DS. LC-MS/MS analysis of [8-13CH]-5DS in root exu3 3 dates after feeding [1-13CH]-CLA. MRM chromatograms (left) and full3 scan spectra of fragment ions (right). The MRM chromatograms of authentic 5DS (red: 331.15/217.00, blue: 331.15/97.00, green: 331.15/ 234.00, m/z in positive mode) and [8-13CH]-5DS (red: 332.15/218.00, 3 blue: 332.15/97.00, green: 332.15/235.00, m/z in positive mode) are shown. B) Conversion of [1-13CH]-CLA to [1-13CH]-18-OH-CLA. LC-MS/ 3 3 MS analysis of [1-13CH]-18-OH-CLA in root exudates after feeding 3 [1-13CH]-CLA. MRM chromatograms (left) and full-scan spectra of frag3 ment ions (right). Authentic 18-OH-CLA was prepared by feeding CL to recombinant Os900. MRM chromatograms of authentic 18-OH-CLA (red: 347.00/303.00, blue: 347.00/113.00, m/z in negative mode) and [1-13CH]-18-OH-CLA (red: 348.00/304.00, blue: 348.00/113.00, m/z in 3 negative mode) are shown. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)