Fig. 4 in Salicornolides A-C from Gracilaria salicornia attenuate pro-inflammatory 5- lipoxygense: Prospective natural anti-inflammatory leads

Fig. 4. Closer image of molecular docking interactions of salicornolides A-C (macrocyclic lactones 1–3) (A–C) and arachidonic acid (D) in the 5-LOX catalytic site as obtained by docking experiment. The docking analysis of salicornolide A (analogue 1) and pro-inflammatory 5-LOX showed three hydrogen-bonds (displayed as red and blue-coloured lines with amino-acyl residues of ASP442 and Arg438 with bond distances of 2.870, 3.139 and 4.077 Å) in the binding site. Salicornolide B displayed five hydrogen-bonds with the amino acid residues of LYS254, ASP290, ARG438 and ARG520, with molecular distances of 2.702, 2.914, 3.435, 3.049, and 2.393 Å. Salicornolide C showed two hydrogen-bonds with amino acid residues of ASP285 and LEU244, with molecular distances of 3.172 and 2.846 Å. The arachidonic showed two hydrogen-bonded interactions with amino acid residues of ARG438 and ARG520, with molecular distances of 2.785 and 2.820 Å. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)