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FIGURES 11–12 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 11–12. Cratera obsidiana sp. nov.: (11) copulatory apparatus of the holotype in sagittal section; (12) copulatory apparatus of the paratype MZU PL. 271 in horizontal section.

FIGURES 13–14 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 13–14. Luteostriata subtilis sp. nov. in dorsal view: (13) color pattern of holotype; (14) eye pattern at the anterior end of paratype MZU PL. 273.

FIGURE 22 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURE 22. Luteostriata subtilis sp. nov., composite reconstruction of the copulatory apparatus of the holotype from sagittal sections. Arrows indicate oblique folds at the level of the gonopore.

FIGURES 2–8 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 2–8. Cratera obsidiana sp. nov., holotype in transverse (2–6) and sagittal sections with the anterior end to the left (7, 8): (2) cephalic region; (3) detail of body-margin in pre-pharyngeal region; (4) pre-pharyngeal region; (5) detail of the dorsal surface in the pre-pharyngeal region; (6) detail of the ventral surface in the pre-pharyngeal region; (7) pharynx; (8) ovary.

FIGURE 1 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURE 1. Cratera obsidiana sp. nov.: dorsal aspect and eye pattern of fixed specimen (paratype MZU PL. 272).

FIGURES 9–10 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 9–10. Cratera obsidiana sp. nov., composite reconstruction of the copulatory apparatus: (9) holotype in sagittal sections; (10) paratype MZU PL. 271 in horizontal sections. Anterior end to the left.

FIGURES 15–21 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 15–21. Luteostriata subtilis sp. nov., holotype in transverse (15–19) and sagittal sections with the anterior to the left (20, 21): (15, 16) cephalic region; (17) pre-pharyngeal region; (18) detail of the dorsal surface in the pre-pharyngeal region; (19) detail of the ventral surface in the pre-pharyngeal region; (20) pharynx; (21) ovary.

FIGURES 23–24 in Two new land planarian species (Platyhelminthes: Tricladida: Geoplanidae) from protected areas in southern Brazil

FIGURES 23–24. Luteostriata subtilis sp. nov.: (23) copulatory apparatus of the holotype in sagittal section; (24) copulatory apparatus of the paratype MZU PL. 273 in sagittal section.

Fig. 4 Choeradoplana iheringi Graff 1899. a in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 4 Choeradoplana iheringi Graff 1899. a Diagrammatic representation of the copulatory apparatus of specimen MZUSP PL 651 from sagittal sections. b Photomicrograph of a sagittal section of the copulatory apparatus of specimen MZUSP PL 512. c Photomicrograph

Fig. 6 in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 6 Choeradoplana pucupucu sp. n. holotype. a Diagrammatic representation of the copulatory apparatus. b Photomicrograph of a sagittal section of the copulatory apparatus. c Photomicrograph of a

Fig. 3 Choeradoplana iheringi Graff 1899. a in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 3 Choeradoplana iheringi Graff 1899. a Specimen MZUSP PL 651 creeping in dorsal view, about 45 mm in length. b Specimen MZUSP PL 519 in dorsal view. about 40 mm in length. c Diagrammatic representation of the pharynx of specimen MZUSP PL 651 in lateral

Fig. 5 in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 5 Choeradoplana pucupucu sp. n. holotype. a Creeping, in dorsal view, about 45 mm in length. b Creeping, in lateral view. c Photomicrograph of a sagittal section of the pharynx. (The high version of this figure can be found in the Electronic Supplementary Material)

Fig. 10 in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 10 Phylogenetic tree of Choeradoplana projected on the map (adapted from Fig. 2). Dashed lines indicate the lineages incongruent with vicariance hypothesis. (The high version of this figure can be found in the Electronic Supplementary Material)

Fig. 9 in Rolling into the deep of the land planarian genus Choeradoplana (Tricladida, Continenticola, Geoplanidae) taxonomy

Fig. 9 Choeradoplana agua sp. n. holotype. a Diagrammatic representation of the copulatory apparatus. b Photomicrograph of a sagittal section of the copulatory apparatus. c Photomicrograph of a sagittal section of the intrabulbar portion of prostatic vesicle.

Figure 6 in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 6. Dugesia xeropotamica. Photomicrographs of sagittal histological sections. A, paratype GAS Pla 29.1, showing the bursal canal with the ectal reinforcement. B, holotype RMNH.VER.21534.1, showing the extrabulbar penial glands and the penis bulb. Abbreviations: bc, bursal canal; cb, copulatory bursa; cm, circular muscle; e, epithelium; epg, extra bulbar penial glands; er, ectal reinforcement; lm, longitudinal muscle; pb, penis bulb.

Figure 5. Dugesia xeropotamica, holotype RMNH.VER.21534.1. A in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 5. Dugesia xeropotamica, holotype RMNH.VER.21534.1. A, sagittal reconstruction of the copulatory apparatus (anterior to the left). B, photomicrograph showing, in a sagittal histological section, the penis bulb with seminal vesicle, penis papilla with the pointed diaphragm, ejaculatory duct, and extrabulbar glands. Abbreviations: bc, bursal canal; cb, copulatory bursa; d, diaphragm; ed, ejaculatory duct; epg, extra bulbar penial glands; er, ectal reinforcement; g, gonopore; ga, genital atrium; lod, left oviduct; lvd, left vas deferens; pb, penis bulb; pp, penis papilla; rod, right oviduct; sg, shell glands; sv, seminal vesicle.

Figure 1. A in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 1. A, sampling sites and geographical distribution of several species of Dugesia on Corsica and Sardinia, including records detailed in the present paper. The rectangular inset marked with C on Sardinia corresponds to panel C. B–D, rectangular insets show: Montecristo Island, in the Tuscany Archipelago (B); confluence of the Bunnari and Mascari rivers, near Sassari, Sardinia (C); and islet of Molara, Sardinia (D).

Figure 4 in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 4. Dugesia xeropotamica. Photomicrograph of a preserved sexual specimen (anterior to the top) from Seccu River (Montegrosso, locality 20, Corsica). Abbreviations: ca, copulatory apparatus; ph, pharynx.

Figure 3. A in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 3. A, schematic representation summarizing the guided tree used for BPP analysis. Results based on multilocus data using two algorithms (A0 and A1), three datasets (I, II, and III), and four evolutionary models (prior combinations) are shown. B, Bayes factor delimitation results for the array of models tested (M1–M5), with different a priori species-delimitation hypotheses.

Figure 2. A, B in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 2. A, B, simplified phylogenetic trees based on dataset III (concatenation of mitochondrial and nuclear data), depicting the conflicting phylogenetic position of Dugesia hoidi. C, detail of Bayesian phylogenetic tree based on dataset III, showing the phylogenetic positions of the unidentified specimens of Molara (C1) and Montecristo (C2). The complete phylogenetic trees inferred from all datasets are available in the Supporting Information (Fig. S1). D, unrooted phylogenetic tree based on dataset III, with valid nominal species and candidate species (PSC1) highlighted in various colours. Bootstrap support (bs), posterior probability (pp), ultrafast bootstrap (UFboot), and SH-aLRT test support values are colour-coded to indicate whether a node is unsupported (white), supported (grey), or fully supported (black).