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FIG. 6 in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 6. ˗ Claviradulomyces vernoniae sp. nov. (VIC 42847, holotype): A, Vernonia sp. defoliated stem with abnormal lenticels colonized by C. vernoniae; B, closeup of abnormal lenticels; C, D, pycnidium with rostrate ostiole neck; E, detail of conidium. Scale bars: C, D, 30 µm; E, 10 µm.

FIG. 5 in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 5. — Claviradulomyces tabebuiae sp. nov. (VIC 42848, holotype): A, Tabebuia roseo-alba (Ridl.) Sandwith stem with abnormal lenticels colonized by C. tabebuiae sp. nov.; B, pycnidium with rostrate-vermiform ostiole neck; C, group of conidia. Scale bars: B, 30 µm; C, 10 µm.

FIG. 4 in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 4. — Claviradulomyces schini sp. nov. (VIC 42845, holotype): A. Schinus terebinthifolia Raddi. Individual branch with dieback symptom and abnormal lenticels colonized by C. schini; B, close-up of hypertrophyed lenticels; C, conidiophores with immature conidia still attached; D, conidia; E, pycnidium with sinuose and rostrate-vermiform ostiole neck. Scale bars: B, C, 10 µm; E, 30 µm.

FIG. 3. — Claviradulomyces machaeriae D.M.Macedo & R.W in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 3. — Claviradulomyces machaeriae D.M.Macedo & R.W.Barreto, sp. nov.: A, branch of Machaerium sp. showing abnormal lenticels colonized by C. machaeriae sp. nov. (VIC 42840); B, C, pycnidia on lenticels. D, E, pycnidia; F, group of immature conidia attached to the conidiogenous cells; G, conidium. Scale bars: B-E 20 µm; F, G, 10 µm.

FIG. 2. — Claviradulomyces casearia L.L.Duarte, D.M.Macedo & R.W in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 2. — Claviradulomyces casearia L.L.Duarte, D.M.Macedo & R.W.Barreto sp. nov. (VIC 42849, holotype): A, B, Casearia ulmifolia Vahl ex. Vent. branches with abnormal lenticels colonized by C. casearia; C, pycnidium with rostrate ostiole neck; D, conidiophores with attached imature conidia. Scale bars: C, 20 µm; D, 10 µm.

FIG. 1 in Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil

FIG. 1. — Phylogenetic tree of the Claviradulomyces isolates inferred by Bayesian analysis of nuclear LSU and rpb2 sequences. Bayesian posterior probabilities are given at the nodes and the accession numbers are presented together with the species names. The newly proposed family Claviradulomyceae is indicated. The tree is rooted with Pleopsidium chlorophanum (Wahlenb.) Zopf and Acarospora laqueata Stizenb. ex Flagey.

Figure 3. Haemoproteus multivacuolatus n in Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species

Figure 3. Haemoproteus multivacuolatus n. sp. (lineage hBUBT1) from the blood of the Common buzzard Buteo buteo: a–d – young gametocytes, e–h – macrogametocytes, i–p – microgametocytes. Long simple arrows – nuclei of parasites. Short simple arrows – vacuoles. Simple arrowhead – pigment granules. Triangle arrowheads – volutin granules. Note that due to marked vacuolisation, the cytoplasm of macrogametocytes stains relatively pale and looks similar to microgametocytes based on the intensity of staining. Giemsa-stained thin blood films. Scale bar = 10 µm. All images were from the hapantotype preparation.

Figure 2 in Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species

Figure 2. Haemoproteus nisi (lineage hCIAE08) from the blood of Western marsh harrier Circus aeruginosus: a – young gametocytes, b–h – macrogametocytes, i–l – microgametocytes. Long simple arrows – nuclei of parasites. Short simple arrows – vacuoles. Simple arrowhead – pigment granules. Triangle arrowheads – clamps of volutin. Simple wide long arrows – spaces between gametocytes and erythrocyte nuclei. Giemsa-stained thin blood films. Scale bar = 10 µm.

Figure 9 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 9. Phylogenetic tree of Rickettsiales bacterium Ac37b, Rickettsia bellii, Rickettsia raoultii, Anaplasma and Coxiella in ticks based on neighbor-joining (NJ) modeling; only values higher than 60 were added to the tree branches. (a) Phylogenetic tree of Rickettsiales bacterium Ac37b identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1320 bp) and triangles (1430 bp). (b) Phylogenetic tree of Rickettsia bellii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1109 bp). (c) Phylogenetic tree of Rickettsia raoultii identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (855 bp). (d) Phylogenetic tree of Anaplasma identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1455 bp) and triangles (547 bp). (e) Phylogenetic tree of Coxiella identified in Inner Mongolia; the 16S rRNA gene sequences obtained in this study are marked with black squares (1463 bp).

Figure 6 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 6. PCoA of β-diversity measures for twelve groups. Weighted UniFrac PCoA graph showing PC1, which accounts for 47.46% of variation, and PC2, which accounts for 28.93% of variation. Different colored dots represent different regions and species.

Figure 5 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 5. (a) Clustering tree analysis by linear discriminant analysis effect size (LEfSe). (b) Histogram of LDA analysis.

Figure 4 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 4. Alpha diversity measures for Dermacentor nuttalli and Ixodes persulcatus in four areas. (a) Shannon's index. (b) Simpson's index.

Figure 1 in Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species

Figure 1. Haemoproteus nisi (lineage hACCNIS08) from the blood of Eurasian sparrowhawk Accipiter nisus: a–d – macrogametocytes, e–h – microgametocytes. Long simple arrows – nuclei of parasites. Short simple arrows – vacuoles. Simple arrowhead – pigment granules. Triangle arrowheads – volutin granules. Simple wide long arrows – spaces between gametocytes and erythrocyte nuclei. Giemsa-stained thin blood films. Scale bar = 10 µm.

Figure 5. Bayesian Inference tree calculated with complete cox1 in Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species

Figure 5. Bayesian Inference tree calculated with complete cox1 (1428 bp), cox3 (753 bp), and cytb (1127 bp) sequences of haemosporidian parasites and Klossiella equi (MH203050) and Klossia razorbacki (MT084562) as the outgroup. Bayesian posterior probabilities and Maximum Likelihood bootstrap values are indicated at most nodes. The scale bar indicates the expected number of substitutions per site according to the model of sequence evolution applied.

Figure 4 in Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species

Figure 4. Median-Joining DNA haplotype network showing the host and geographic distribution of six Haemoproteus nisi group lineages (478 bp cytb sequences) found in accipitriform raptors from Austria and France.

Figure 7 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 7. Venn diagram for cluster analysis of OTUs between Dermacentor nuttalli and Ixodes persulcatus (genus level).

Figure 3 in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 3. Microbe composition of different regions and species. (a) Top 10 microbial components at the genus level. (b) Top 12 microbial components at the species level.

Figure 2. Shannon–Wiener curve. X in Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China

Figure 2. Shannon–Wiener curve. X-axis: amount of sequencing data; Y-axis: corresponding Shannon diversity index.

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Figure 3. SDS PAGE and Western blots with rEnSerp1 in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Figure 3. SDS PAGE and Western blots with rEnSerp1, ESP and CWE. Lines 1–3, 1D gel. Lines 4–10, Western blot. M, protein standard; 1, rEnSerp1; 2, ESP; 3, CWE sample; 4, rEnSerp1 with anti-HIS antibodies; 5, rEnSerp1 without primary antibodies; 6, rEnSerp1 with anti-rEnSerp1 sera; 7, rEnSerp1 with pre-immune sera; 8, ESP with anti-rEnSerp1 sera; 9, ESP with pre-immune sera; 10, CWE with anti-rEnSerp1 sera. Arrow points to the expected recombinant EnSerp1 band. Arrowhead points to the natural form of EnSerp1 in the ESP sample. Dots indicate fragmented parts of rEnSerp1.