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Supplementary material 2 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae). Deutsche Entomologische Zeitschrift 67(2): 197-207. https://doi.org/10.3897/dez.67.56163

Neighbour-joining topology

opencc-zeroOct 2020View details →
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Figure 2 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae). Deutsche Entomologische Zeitschrift 67(2): 197-207. https://doi.org/10.3897/dez.67.56163

Figure 2 Maximum statistical parsimony networks of the species pairs. A.Agonum micans (Nicolai, 1822) (blue) and Agonum scitulum Dejean, 1828 (red); B.Agonum impressum (Panzer, 1796) (yellow) and Agonum sexpunctatum (Linne, 1758) (green) and C.Agonum duftschmidi Schmidt, 1994 (violet) and Agonum emarginatum (Gyllenhal, 1827) (light brown). Used parameters included default settings for connection steps, gaps were treated as fifth state. Each line represents a single mutational change, whereas small black dots indicate missing haplotypes. The numbers of analysed specimens (n) are listed, whereas the diameter of the circles is proportional to the number of haplotypes sampled (see given open circles with numbers). Scale bars: 1 mm. Beetle images were obtained from http://www.eurocarabidae.de.

opencc-by-4.0Oct 2020View details →
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Figure 3 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae). Deutsche Entomologische Zeitschrift 67(2): 197-207. https://doi.org/10.3897/dez.67.56163

Figure 3 Neighbour-joining (NJ) topology of the analysed ground beetle species, based on Kimura 2-parameter distances. Triangles show the relative number of individual's sampled (height) and sequence divergence (width). Numbers next to nodes represent non-parametric bootstrap values > 75% (1,000 replicates). Beetle images were obtained from http://www.eurocarabidae.de except for Agonum monachum (Duftschmid, 1812) (Photo taken by Lars Hendrich).

opencc-by-4.0Oct 2020View details →
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Figure 1 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae). Deutsche Entomologische Zeitschrift 67(2): 197-207. https://doi.org/10.3897/dez.67.56163

Figure 1 Representative images of analysed Agonum species. A.Agonum (Olisares) duftschmidi Schmidt, 1994; B.Agonum (Olisares) ericeti (Panzer, 1809); C.Agonum (Olisares) hypocrita (Apfelbeck, 1904); D.Agonum (Olisares) sexpunctatum (Linné, 1758); E.Agonum (Agonum) marginatum (Linné, 1758); F.Agonum (Agonum) muelleri (Herbst, 1784); G.Agonum (Europhilus) fuliginosum (Panzer, 1809); H.Agonum (Europhilus) piceum (Linné, 1758) and I.Agonum (Europhilus) thoreyi Dejean, 1828. Scale bars: 1 mm. All images were obtained from http://www.eurocarabidae.de.

opencc-by-4.0Oct 2020View details →
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Supplementary material 1 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae). Deutsche Entomologische Zeitschrift 67(2): 197-207. https://doi.org/10.3897/dez.67.56163

Barcode analysis using the BOLD workbench

opencc-zeroOct 2020View details →
zenodo28/100

Supplementary material 1 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Barcode analysis using the BOLD workbench

opencc-zeroNov 2020View details →
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Figure 4 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Figure 4 Subtree of the Neighbor-joining topology based on Kimura 2-parameter distances of all analyzed specimens of Pterostichus panzeri (Panzer, 1805) and nearest neighbor. Branches with specimen ID-number from BOLD, species names and sample localities. Numbers next to internal nodes are non-parametric bootstrap values (in %). Cluster (A-C) with BINs (if available) based on the barcode analysis from 11-05-2020. Beetle image was obtained from www.eurocarabidae.de.

opencc-by-4.0Nov 2020View details →
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Supplementary material 2 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Neighbor-joining topology

opencc-zeroNov 2020View details →
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Figure 3 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Figure 3 Maximum statistical parsimony networks of the sibling species pairs APterostichus nigrita Paykull, 1790 (green) and Pterostichus rhaeticus Heer, 1837 (blue) and BPterostichus adstrictus Eschscholtz, 1823 (yellow) and Pterostichus oblongopunctatus (Fabricius, 1787) (red). Used parameters included default settings for connection steps, gaps were treated as fifth state. Each line represents a single mutational change whereas small black dots indicate missing haplotypes. The numbers of analyzed specimens (n) are listed, whereas the diameter of the circles is proportional to the number of haplotypes sampled (see given open circles with numbers). Scale bars 1 mm. Beetle images were obtained from www.eurocarabidae.de except Pterostichus adstrictus (photographer: Ditta Balke).

opencc-by-4.0Nov 2020View details →
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Figure 2 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Figure 2 Neighbor-joining (NJ) topology of the analyzed ground beetle species based on Kimura 2-parameter distances. Triangles show the relative number of individual's sampled (height) and sequence divergence (width). Red triangles indicate species with intraspecific maximum pairwise distances > 2.2%, blue triangles species pairs with interspecific distances < 2.2%. Numbers next to nodes represent non-parametric bootstrap values > 90% (1,000 replicates). Images are provided for species recorded in Germany whereas asterisks indicate species not recorded in Germany. All beetle images were obtained from www.eurocarabidae.de except of Poecilus sericeus (photographer: Katja Neven, Lars Hendrich).

opencc-by-4.0Nov 2020View details →
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Figure 1 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Figure 1 Representative images of analyzed beetle species AAbax parallelepipedus (Piller & Mitterbacher, 1783) BMolops piceus (Panzer, 1793) CPoecilus versicolor (Sturm, 1824) DPterostichus (Eosteropus) aethiops (Panzer, 1796) EPterostichus (Omaseus) melanarius (Illiger, 1798) FPterostichus (Oreophilus) multipunctatus (Dejean, 1828) GPterostichus (Bothriopterus) quadrifoveolatus Letzner, 1852 HPterostichus (Argutor) vernalis Panzer, 1796 and IStomis pumicatus (Panzer, 1795). Scale bars: 1 mm. All images were obtained from www.eurocarabidae.de.

opencc-by-4.0Nov 2020View details →
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Figure 5 from: Raupach MJ, Hannig K, Morinière J, Hendrich L (2020) A DNA barcode library for ground beetles of Germany: the genus Pterostichus Bonelli, 1810 and allied taxa (Insecta, Coleoptera, Carabidae). ZooKeys 980: 93-117. https://doi.org/10.3897/zookeys.980.55979

Figure 5 Subtree of the Neighbor-joining topology based on Kimura 2-parameter distances of all analyzed specimens of Molops piceus (Panzer, 1793) and nearest neighbor. Branches with specimen ID-number from BOLD, species names and sample localities. Numbers next to internal nodes are non-parametric bootstrap values (in %). Cluster (A-C) with BINs based on the barcode analysis from 11-05-2020. Beetle image was obtained from www.eurocarabidae.de.

opencc-by-4.0Nov 2020View details →
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Fig 6 in A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library

Fig 6. Neighbour-joining tree showing all available DNA barcodes for species in family Vespertilionidae reported from Peninsular Malaysia. The percentage of pseudoreplicate trees (±70%) in which the DNA barcodes clustered together in the bootstrap test (500 pseudoreplicates) are shown above the branches. Abbreviation as follows: PM = Peninsular Malaysia, VN = Vietnam, BN = Borneo (including Sabah & Sarawak of East Malaysia, Brunei and Kalimantan Indonesia), TH = Thailand, LA = Laos, SM = Sumatera Indonesia, JV = Java Indonesia, CH = China, CM = Cambodia. https://doi.org/10.1371/journal.pone.0179555.g006

opencc-by-4.0Jul 2017View details →
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Fig 1 in A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library

Fig 1. Bat species with recent (dated during or after the year 2000) and old (dated before year 2000) records from Peninsular Malaysia. https://doi.org/10.1371/journal.pone.0179555.g001

opencc-by-4.0Jul 2017View details →
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Supplementary material 3 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure S1. Maximum Likelihood tree inferred from aligned COI mtDNA amino acids sequences.

opencc-zeroDec 2020View details →
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Figure 4 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure 4 Ancyronyx pulcherrimusA male from Gunung Mulu NP, body length 1.82 mm B brachypterous male from Gunung Mulu NP, dorsal view (left elytron and apical segments of abdomen removed), body length 1.92 mm C male from Bayur River, dorsal view, body length 1.70 mm D male [JK106] from Bayur River, dorsal view, body length 1.70 mm.

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Figure 7 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure 7 Ancyronyx lianlabangorum sp. nov. A ovipositor, paratype [JK145], ventral view B male sternite IX, holotype, ventral view C female sternite VIII, paratype [JK145], ventral view. Scale bars: 0.1 mm.

opencc-by-4.0Dec 2020View details →
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Figure 3 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure 3 Ancyronyx lianlabangorum sp. nov. A holotype, dorsal view, body length 2.97 mm B female paratype, dorsal view, body length 2.97 mm C holotype, dorsal view of body D holotype, ventral view of body E brachypterous paratype with removed elytron, dorsal view, body length 2.98 mm.

opencc-by-4.0Dec 2020View details →
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Figure 2 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure 2 Results of the PCA analysis showing ordination of Ancyronyx lianlabangorum sp. nov. and A. pulcherrimus Kodada, Jäch & Čiampor specimens along the first two principal components A males B females. Clusters of A. pulcherrimus specimens on left side of plot (yellow lines), clusters of A. lianlabangorum sp. nov. on right side (green lines). Yellow circles: A. pulcherrimus specimens from Gunung Mulu NP, first lineage; blue circles: A. pulcherrimus from Bayur River, second lineage; brown circles: A. pulcherrimus from Bayur River, third lineage; black circles: A. pulcherrimus from Bayur River, specimens not barcoded, not assignable to the first or second lineage according to morphology; red circle: A. pulcherrimus from Brunei; green squares: A. lianlabangorum sp. nov. Genetic voucher IDs are included according to Table 1.

opencc-by-4.0Dec 2020View details →
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Figure 6 from: Kodada J, Jäch MA, Freitag H, Čiamporová-Zaťovičová Z, Goffová K, Selnekovič D, Čiampor Jr F (2020) Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae). ZooKeys 1003: 31-55. https://doi.org/10.3897/zookeys.1003.55541

Figure 6 Ancyronyx lianlabangorum sp. nov., aedeagus of holotype A ventral view B dorsal view C lateral view. Scale bar: 0.1 mm.

opencc-by-4.0Dec 2020View details →

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