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278
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ShareScore release 0.9.0
Dataset results
278 results for “cytometry”
The impact of deep space radiation on cognitive performance: From biological sex to biomarkers to countermeasures (Flow Cytometry, Blood)
In the coming decade, astronauts will travel back to the moon in preparation for future Mars missions. Exposure to galactic cosmic radiation (GCR) is a major obstacle for deep space travel. Using multivariate principal components analysis, we found sex dimorphic responses in mice exposed to accelerated charged particles to simulate GCR (GCRsim); males displayed impaired spatial learning, whereas females did not. Mechanistically, these GCRsim induced learning impairments corresponded with chronic microglia activation and synaptic alterations in the hippocampus. Temporary microglia depletion shortly after GCRsim exposure mitigated GCRsim induced deficits measured months after the radiation exposure. Furthermore, blood monocyte levels measured early after GCRsim exposure were predictive of the late learning deficits and microglia activation measured in the male mice. Our findings (i) advance our understanding of charged particle induced cognitive challenges, (ii) provide evidence for early peripheral biomarkers for identifying late cognitive deficits, and (iii) offer potential therapeutic strategies for mitigating GCR induced cognitive loss. This study derives results from the Flow Cytometry assay using blood. These data are related to OSD-479 (behavior assays), OSD-777 (Microglia), and OSD-778 (Synapse).
The impact of deep space radiation on cognitive performance: From biological sex to biomarkers to countermeasures (Flow Cytometry, Microglia)
In the coming decade, astronauts will travel back to the moon in preparation for future Mars missions. Exposure to galactic cosmic radiation (GCR) is a major obstacle for deep space travel. Using multivariate principal components analysis, we found sex dimorphic responses in mice exposed to accelerated charged particles to simulate GCR (GCRsim); males displayed impaired spatial learning, whereas females did not. Mechanistically, these GCRsim induced learning impairments corresponded with chronic microglia activation and synaptic alterations in the hippocampus. Temporary microglia depletion shortly after GCRsim exposure mitigated GCRsim induced deficits measured months after the radiation exposure. Furthermore, blood monocyte levels measured early after GCRsim exposure were predictive of the late learning deficits and microglia activation measured in the male mice. Our findings (i) advance our understanding of charged particle induced cognitive challenges, (ii) provide evidence for early peripheral biomarkers for identifying late cognitive deficits, and (iii) offer potential therapeutic strategies for mitigating GCR induced cognitive loss. This study derives results from the Flow Cytometry assay using microglia. These data are related to OSD-479 (behavior assays), OSD-776 (Blood), and OSD-778 (Synapse).
The impact of deep space radiation on cognitive performance: From biological sex to biomarkers to countermeasures (Flow Cytometry, Synapse from Hippocampus)
In the coming decade, astronauts will travel back to the moon in preparation for future Mars missions. Exposure to galactic cosmic radiation (GCR) is a major obstacle for deep space travel. Using multivariate principal components analysis, we found sex dimorphic responses in mice exposed to accelerated charged particles to simulate GCR (GCRsim); males displayed impaired spatial learning, whereas females did not. Mechanistically, these GCRsim induced learning impairments corresponded with chronic microglia activation and synaptic alterations in the hippocampus. Temporary microglia depletion shortly after GCRsim exposure mitigated GCRsim induced deficits measured months after the radiation exposure. Furthermore, blood monocyte levels measured early after GCRsim exposure were predictive of the late learning deficits and microglia activation measured in the male mice. Our findings (i) advance our understanding of charged particle induced cognitive challenges, (ii) provide evidence for early peripheral biomarkers for identifying late cognitive deficits, and (iii) offer potential therapeutic strategies for mitigating GCR induced cognitive loss. This study derives results from the Flow Cytometry assay using synapses from hippocampus. These data are related to OSD-479 (behavior assays), OSD-776 (Blood), and OSD-777 (Microglia).
Combined space stressors induce independent behavioral deficits predicted by early peripheral blood monocytes (flow cytometry)
Interplanetary space travel poses many hazards to the human body. To protect astronaut health and performance on critical missions, there is first a need to understand the effects of deep space hazards, including ionizing radiation, confinement, and altered gravity. Previous studies of rodents exposed to a single such stressor document significant deficits, but our study is the first to investigate possible cumulative and synergistic impacts of simultaneous ionizing radiation, confinement, and altered gravity on behavior and cognition. Our cohort was divided between 6‐month‐old female and male mice in group, social isolation, or hindlimb unloading housing, exposed to 0 or 50 cGy of 5 ion simplified simulated galactic cosmic radiation (GCRsim). We report interactions and independent effects of GCRsim exposure and housing conditions on behavioral and cognitive performance. Exposure to GCRsim drove changes in immune cell populations in peripheral blood collected early after irradiation, while housing conditions drove changes in blood collected at a later point. Female mice were largely resilient to deficits observed in male mice. Finally, we used principal component analysis to represent total deficits as principal component scores, which were predicted by general linear models using GCR exposure, housing condition, and early blood biomarkers. This dataset derives results from the flow cytometry assay using blood samples from same source animals used for behavioral studies in OSD-618.
Interleukin-18 induced chromatin accessibility coupled to proteomic analysis by mass cytometry and ion beam imaging
GEO Series GSE124297. Homo sapiens. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Single-cell mass cytometry and transcriptomic profiling of circulating immune cells identifies an expansion of CD25hi switched memory B cells in Osteoarthritis
GEO Series GSE253539. Homo sapiens. 1 samples. Type: Expression profiling by high throughput sequencing.
Human Monocyte Heterogeneity as Revealed by High-Dimensional Mass Cytometry
GEO Series GSE146447. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Quantitative assessment of transcriptomic differences between ASPcKO and ASPcKOP7cKO flow cytometry-sorted murine FN-RMSs by RNA sequencing
GEO Series GSE166808. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Pooled CRISPR Screening of High-content Cellular Phenotypes by Ghost Cytometry_03
<p>Pooled CRISPR Screening of High-content Cellular Phenotypes by Ghost Cytometry (Ghost Cytometer data)</p>
SCITO-seq: single-cell combinatorial indexed cytometry sequencing
GEO Series GSE147808. Homo sapiens; Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
Using Mass Cytometry and In vivo Imaging to Study Emergency Repopulation of Immune Cells in Murine Liver
GEO Series GSE81645. Mus musculus. 12 samples. Type: Other.
Dataset related to article "Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer"
<p><strong>Abstract</strong></p><p>Liquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a cohort of >750 patients with clinically localized PCa. The overall SF collection yield was 18.2% (39% when considering only compliant patients), with about a half of the patients (53.15%) not consenting to SF donation. Independent favorable predictors for SF collection were younger age and lower prostate volume. We implemented a protocol to enrich prostate-derived cells by multi-color flow cytometry and applied it on SF and urine samples from 100 patients. The number of prostate-enriched cells (SYTO-16+ PSMA+ CD45-) was variable, with higher numbers of cells isolated from SF than urine (<i>p</i> value < 0.001). Putative cancer cells (EpCAMhigh) were 2% of isolated cells in both specimens. The fraction of EpCAMhigh cells over prostate-enriched cells (PSMA+) significantly correlated with patient age in both semen and urine, but not with other clinical parameters, such as Gleason Score, ISUP, or TNM stage. Hence, enumeration of prostate-derived cells is not sufficient to guide PCa diagnosis; additional molecular analyses to detect patient-specific cancer lesions will be needed</p>
Immunogenomics of colorectal cancer response to immune checkpoint blockade: Imaging Mass Cytometry
<p><strong>Immunogenomics of colorectal cancer response to immune checkpoint blockade</strong></p> <p>Imaging Mass Cytometry data supporting the paper: "Immunogenomics of colorectal cancer response to immune checkpoint blockade"</p> <p>The provided data includes:</p> <ul> <li>Sample metadata: Raw_txt_mcd_files.tar.gz/Metadata.csv</li> <li>Raw ablation data in .mcd or .txt format: Raw_txt_mcd_files.tar.gz</li> <li>Thresholded and cleaned tiff images:Cleaned_Tiff_Images.tar.gz</li> <li>Tissue/tumor/stroma masks: Masks.tar.gz</li> <li>Single-cell marker expression values and centroid coordinates: Single_Cell_Data.csv</li> </ul>
Multi-omics and imaging mass cytometry characterization of human kidneys to identify pathways and phenotypes associated with kidney damage
GEO Series GSE217427. Homo sapiens. 44 samples. Type: Expression profiling by high throughput sequencing.
Flow cytometry gating 2D: x (SangerP2)
<p>Processed data set.</p>
Flow cytometry gating 2D: x (HIPC, pregnancy), y (HIPC, Pregnancy, sangerP2), nD x (pregnancy), nD y (pregnancy, HIPC, sangerP2)
<p>Processed data set.</p>
Flow cytometry gating 2D: nD x (HIPC, sangerP2.2)
<p>Processed data set.</p>
Flow cytometry gating 2D: nD x (sangerP2.1)
<p>Processed data set.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.