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481 results for “freezing”
Transcriptional analysis of spleens from mice preserved with the Rapid Freeze hardware
Data from the NASA Rodent Research-1 (RR-1) mission showed that gene-expression levels in mouse livers are different depending on what tissue preservation protocol is used and that slow freezing is not an effective method for preserving signals in gene-expression data. In response to these and other observations the Rapid Freeze hardware was built for use on the International Space Station. The Rapid Freeze hardware freezes mouse tissues (Glovebox freezer) and whole carcasses (Cryochiller) at rates closely mimicking those attained with immersion in liquid nitrogen. Because this hardware will be used extensively on future rodent research missions it is crucial to understand whether or not it preserves signals in gene expression data in order to maximize the value of these rare and expensive spaceflight experiments. Therefore this study was designed with three goals: 1) To evaluate the temperature profile of the Cryochiller and Glovebox freezer cartridges (Rapid Freeze hardware) over time during mock on-orbit procedures; 2) To determine the freezing profiles of tissues and carcasses using Rapid Freeze hardware at both optimal and sub-optimal temperatures (to mimic on-orbit operations) compared with those frozen in liquid nitrogen (the laboratory gold standard) or frozen in at -80 C (the current standard method); 3) To identify gene expression changes in a) tissues that were frozen via the Glovebox freezer and b) tissues dissected from whole or partial carcasses that were frozen via the Cryochiller versus tissues that were frozen via control methods (liquid nitrogen or -80C slow freeze) to assess how the Rapid Freeze hardware compares with laboratory gold standard practices and our current standard methods.
ABoVE: River Ice Breakup and Freeze-up Stages, Yukon River Basin, Alaska, 1972-2016
This dataset provides estimates of river ice breakup and freeze-up stages along selected reaches of the Yukon and Tanana Rivers in the Yukon River Basin in interior Alaska from 1972-2016. Time series of Landsat satellite images were visually interpreted to identify the day of year and characteristics of the different stages of river ice seasonality. The stages of breakup or freeze-up were distinguished from one another based on the spatial extent and patterns of open water and ice cover. Images were displayed as false color composites, with the shortwave infrared (SWIR), near infrared (NIR), and green bands represented by red, green, and blue.
Identification of A Novel bZIP Transcription Factor in Camellia sinensis as A Negative Regulator of Freezing Tolerance in Transgenic Arabidopsis
GEO Series GSE84570. Arabidopsis thaliana. 17 samples. Type: Expression profiling by array.
BMP, MEK and WNT modulation with NGN2 expression for rapid neuron differentiation from hiPSC amenable to regional patterning [BMWi_freeze]
GEO Series GSE253509. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3’ mRNA profiling
GEO Series GSE109535. Homo sapiens; Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Effects of Freeze-Thawing and Intravenous Infusion on Mesenchymal Stromal Cell Gene Expression
GEO Series GSE76081. Homo sapiens; Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
GEO Series GSE78036. Homo sapiens. 6 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
The effect of freeze-thaw cycles on gene expression levels in lymphoblastoid cell lines
GEO Series GSE58942. Homo sapiens. 187 samples. Type: Expression profiling by array.
Gene Expression during Model Dough Fermentation after Freezing Preservation of S. cerevisiae Baker’s Yeast Cells
GEO Series GSE101071. Saccharomyces cerevisiae. 4 samples. Type: Expression profiling by array.
Transcriptomic differences in bovine blastocysts following vitrification and slow freezing at morula stage [EmbryocryovitSF]
GEO Series GSE95379. Bos taurus. 4 samples. Type: Expression profiling by array.
Rat colonic tissue transcriptome with dietary intervention by freeze-dried baby spinach (10% w/w in AIN-93 diet)
GEO Series GSE180160. Rattus norvegicus. 36 samples. Type: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing.
Expression data from the Arabidopsis with freezing treatment following the inhibitors of DNA methylation and histone deacetylation
GEO Series GSE63131. Arabidopsis thaliana. 10 samples. Type: Expression profiling by array.
Role of exogenous abscisic acid in freezing tolerance of mangrove Kandelia obovata under natural frost condition at near 32oN
GEO Series GSE219193. Kandelia obovata. 24 samples. Type: Expression profiling by high throughput sequencing.
The effect and mechanism of freeze-dried powder of Poecilobdella manillensis on improving inflammatory injury of rat glomerular mesangial cells through TXNIP / NLRP3 pathway.
GEO Series GSE270526. Rattus norvegicus. 9 samples. Type: Expression profiling by high throughput sequencing.
Transcriptomic differences in bovine blastocysts following vitrification and slow freezing at morula stage [EmbryocryoVit]
GEO Series GSE95380. Bos taurus. 4 samples. Type: Expression profiling by array.
Genome-wide analysis of the effect of long-term freezing of PAXgene Blood RNA tubes
GEO Series GSE24757. Homo sapiens. 24 samples. Type: Expression profiling by array.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Background: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays. We tested the effect of multiple (1, 2, 3, 4, 5, 10) freeze-thaw cycles on the results of four commonly used immunological assays: haemolysis-haemagglutination titres, haptoglobin concentration, bacterial killing capacity and total immunoglobulins (IgY). We tested five different bird species from four different bird orders (Passeriformes, Columbiformes, Charadriiformes and Galliformes), and we included both captive and free-living individuals. In addition, we tested for haptoglobin concentrations and the haemolysis-haemagglutination assay if re-analysing samples 1 year apart led to different results. For the haemolysis-haemagglutination assay we also tested two different sources of rabbit blood, and we compared untreated microtitre plates with plates that were "blocked" to prevent nonspecific interactions between the plate and assay reagents. Results: Repeated freezing and thawing of plasma had no effect on lysis titres, haptoglobin concentrations, bacterial killing capacity, or total immunoglobulin levels. Agglutination titres were unaffected by up to five cycles but were lower after ten freeze-thaw cycles. For the haemolysis-haemagglutination assay and haptoglobin concentrations, re-analysing samples 1 year apart yielded highly correlated data. For the haemolysis haemagglutination assay,thesourceofrabbit blood did not influence the results, and the untreated vs. blocked plates differed slightly overall, but at the individual level assay results were highly correlated. Using different rabbit blood sources or different types of microtitre plates yielded highly correlated data.Conclusions: Our data suggest that repeated freeze-thaw cycles do not impair assay results to the point of influencing ecological or evolutionary conclusions. Plasma samples can be safely stored in one tube and thawed repeatedly for different assays. Nevertheless, we recommend consistent treatment of samples in terms of freeze-thaw cycles or other laboratory treatments to minimize the potential for introducing a systematic bias.
Hazard Atlas from "Quantifying the Compound Hazard of Freezing Rain and Wind Gusts Across CONUS"
<p>Summary data from the analysis of compound freezing rain-wind gust hazards using Automated Surface Observing Station (ASOS) network data for all hours from January 1, 2005 to December 31, 2022.</p> <p>Note (added 2024-03-06): The SPI index within the Atlas data refers to the SPIA index (https://spia-index.com/), which is copyrighted (https://spia-index.com/copyright.php). Special thanks to Sidney Sperry and Steven Piltz for permission to use the index in this work.</p>
supplemental data for QTL MAPPING TO IDENTIFY LOCI AND CANDIDATE GENES ASSOCIATED WITH FREEZING TOLERANCE TRAIT IN CAMELINA SATIVA
Open the record for dataset details and reuse information.
Numerical data set belonging to: 'Transient Freezing of Water in a Square Duct: An Experimental Benchmark'
<p>This is the numerical data-set belonging to 'Transient Freezing of Water in a Square duct'. The simulation outputs can be opened using the opensource application GMSH.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.