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445 results for “karyotype”
Figure 13 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 13. Karyotypes of the Cylominae, without treatment. A, D, Exydrus gibbosus, mitosis, midgut. B–C, E, Tormissus magnulus, mitosis, midgut. F–H, Rygmodus modestus (F–G, meiotic metaphase I, testes; H, mitotic karyotype, midgut). Habitus figures: (I) Exydrus gibbosus; (J) Rygmodus modestus, from Minoshima et al. (2018).
Figure 14 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 14. Karyotypes of the Omicrini and Sphaeridiini, without treatment. A–F, Omicrini: (A–B) Noteropagus sp. from Taiwan, meiotic karyotypes from testes; (C–D) Omicrogiton insularis, mitotic karyotype, midgut; (E–F) Paromicrus sp. from Taiwan, mitotic karyotype, midgut. G–I, habitus of examined specimens: (G) Noteropagus sp.; (H) Omicrogiton insularis; (I) Paromicrus sp. J–P, Sphaeridiini: Sphaeridium; (J–K) Sphaeridium lunatum (J, testes, mitosis; K, midgut, mitosis); (L) Sphaeridium scarabaeoides, mitosis, midgut; (M) Sphaeridium bipustulatum, mitosis, midgut; (N–P) meiosis, metaphase I, testes: (N) Sphaeridium scarabaeoides; (O) Sphaeridium bipustulatum; (P) Sphaeridium lunatum. Q, habitus of Sphaeridium scarabaeoides.
Figure 12 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 12. Karyotypes of the Cylominae, without treatment. A–B, Adolopus sp., mitosis from midgut. D, Cyloma guttulatus, mitosis from midgut. E–G, Cyloma sp. (E–F, meiotic first metaphase; G, testes, mitosis;). I–M, Cylomissus glabratus: (I–J) mitotic metaphase from midgut; (K–M) meiotic metaphase I from testes. Habitus figures: (C) Adolopus sp.; (H) Cyloma sp., specimen collected with karyotyped voucher; (N) Cylomissus glabratus, from Minoshima et al. (2015).
Figure 11 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 11. Karyotypes of the Acidocerinae. A–C, Agraphydrus, mitotic metaphase, midgut: (A–B) Agraphydrus decipiens; (C) Agraphydrus variabilis. D–L, Helochares: (D–E) Helochares lividus, mitosis, midgut; (F) Helochares obscurus, mitosis, midgut; (G–H) Helochares punctatus, mitosis, midgut; (I) Helochares punctatus, karyotype of male embryo with multiple y-chromosomes; (J–K) meiotic metaphase I from testes (J, Helochares punctatus; K, Helochares lividus); (L) Helochares sauteri, mitosis, midgut. A, D, F, G, I–L, without treatment. B, C, E, H, C-banded. Habitus figures: (M) Agraphydrus decipiens, from Minoshima, Komarek, & Ȏhara 2015; (N) Helochares obscurus.
Figure 10 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 10. Karyotypes of Enochrus (Lumetus). A–C, Enochrus quadripunctatus, mitosis, midgut. D–K, Enochrus fuscipennis, mitosis, midgut (H–I, specimens from Denmark, Rømø Island with the karyotypes indicating their hybrid origin). L–N, meiotic metaphase I from testes (L, Enochrus quadripunctatus, UK: East Walton, Norfolk; M–N, Enochrus fuscipennis, Denmark: Rømø Island). O–P, Enochrus fuscipennis, testes, mitotic metaphase from the same specimens as in (H–I). A, B, D, E, H, I, J, M–P, without treatment. C, F, G, K, L, C-banded.
Figure 9 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 9. Karyotypes of Enochrus (Lumetus), mitosis from embryos. A, Enochrus bicolor. B–C, Enochrus ochropterus. D–E, Enochrus testaceus. F–K, Enochrus halophilus. A, B, D, F, H, I, without treatment. C, E, G, K, C-banded. Habitus figures: (L) Enochrus (Lumetus) testaceus; (M) Enochrus (Lumetus) halophilus.
Figure 8 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 8. Mitotic karyotypes of the Enochrinae. A, Cymbiodyta marginella, embryo. B–D, European usual-looking species of Enochrus (Methydrus) from embryos: (B) Enochrus affinis; (C) Enochrus coarctatus; (D–E) Enochrus nigritus. F–G, unusual species assigned at the moment to Enochrus (Methydrus): (F) Enochrus morenae, midgut; (G) Enochrus sauteri, midgut. H, Enochrus (s.s.) melanocephalus, embryo. A–G, without treatment. H, C-banded. Habitus figures: (I) Cymbiodyta marginella; (J) Enochrus morenae.
Figure 17 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 17. Mitotic karyotypes of Cercyon from midgut. (A) Cercyon marinus; (B–E) Cercyon lateralis; (F–G) Cercyon obsoletus; (H) Cercyon impressus; (I–K) Cercyon haemorrhoidalis; (L–N) Cercyon melanocephalus. A, B, D, F, H, I, K, L, N, without treatment. C, E, G, J, M, C-banded. Habitus figures: (O) Cercyon marinus; (P) Cercyon impressus; (Q) Cercyon haemorrhoidalis, from Fikáček (2019).
Figure 15 in Karyotypes of water scavenger beetles (Coleoptera: Hydrophilidae): new data and review of published records
Figure 15. Karyotypes of the Coelostomatini and Protosternini. A–C, Coelostoma orbiculare, embryo (A, with B-chromosomes; B–C, without B-chromosomes). D–F, Dactylosternum flavicorne, embryo. G, Dactylosternum corbetti, mitosis, midgut. J–K, Protosternum abnormale, meiotic nuclei from testes. A–F, J, K, without treatment. G, C-banded. Habitus figures: (H) Coelostoma orbiculare; (I) Dactylosternum corbetti; (L) Protosternum abnormale, from Fikáček et al. (2018).
Comparative karyotype analysis in chickpea (Cicer arietinum L.) using oligo painting FISH
<p>Chickpea (<i>Cicer arietinum</i> L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of chickpea genome at chromosomal level, including the long-range molecular chromosome organization is limited. Earlier cytogenetic studies in chickpea suffered from limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes <i>in situ</i>. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea<i>. </i>We demonstrate that even draft genome sequences are adequate to develop oligo-FISH barcodes for identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo-FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both, kabuli and desi reference genomes. Thus, oligo painting appears as a powerful tool not only for comparative karyotyping, but also for validation of genome assemblies.</p>
Supplementary material 4 from: She C-W, Jiang X-H, He C-P (2023) Comparative karyotype analysis of eight Cucurbitaceae crops using fluorochrome banding and 45S rDNA-FISH. Comparative Cytogenetics 17(1): 31-58. https://doi.org/10.3897/compcytogen.v17.i1.99236
The correlational analysis between the difference in TCL and the change in nuclear DNA content within the eight Cucurbitaceae crops using the SPSS 25.0 software
Supplementary material 1 from: de Andrade ARM, Cardoso DC, Cristiano MP (2023) Assessing ploidy levels and karyotype structure of the fire ant Solenopsis saevissima Smith, 1855 (Hymenoptera, Formicidae, Myrmicinae). Comparative Cytogenetics 17: 59-73. https://doi.org/10.3897/compcytogen.17.100945
Results from the karyomorphometrical analyses of Solenopsis saevissima and Chromosome counts frequency by individual and colony of Solenopsis saevissima
Figure 1 in Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae)
Figure 1. Distribution of heterochromatin in Ronderosia species. C-banding (A-F) staining. Pictures were obtained from male metaphase I meiocytes. Autosomes with positive signals are indicated with numbers. Green (neo-X) and blue (neo-Y) arrows show positive signals: R. piceomaculata (A), R. malloi (B), R. paraguayensis (C), R. forcipata (D), R. cinctipes (E), R. gracilis (F). Scale bar = 10 μm.
Figure 5 in Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae)
Figure 5. FISH of multigene families in male metaphase I chromosomes of seven Ronderosia species, counterstained with DAPI (blue). Positive signals for U2, H3 and telomere (TTAGG)n probes are indicated with the chromosome number (1-11) for R. piceomaculata (A - C), R. malloi (D - F), R. dubia (G - I), R. paraguayensis (J - L), R. forcipata (M - O), R. cinctipes (P, Q), R. gracilis (R - T). Positive red signals indicate telomere sequence locations. Neo-sex chromosomes (white dotted lines) and metacentric autosomes (1/5) are indicated. The phylogenetic hypothesis shown is from Castillo et al. (2019). Scale bar = 10 μm.
Figure 4 in Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae)
Figure 4. FISH of multigene families in male metaphase I chromosomes of seven Ronderosia species, counterstained with DAPI (blue). Positive signals for 18S and 5S probes are indicated with the chromosome number (1-11) for R. piceomaculata (A, B), R. malloi (C, D), R. dubia (E), R. paraguayensis (F, G), R. forcipata (H, I), R. cinctipes (J), R. gracilis (K, L). Neo-sex chromosomes (white dotted lines) and metacentric autosomes (1/5) are indicated. The phylogenetic hypothesis shown is from Castillo et al. (2019). Scale Bar = 10 μm.
Figure 3 in Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae)
Figure 3. Chromosome location of 18S and 5S in eight Ronderosia species. Grey squares indicate the existence of a certain chromosome whereas white areas represent the absence of that chromosome. Coloured squares indicate different gene locations detected by FISH; light blue (pr = proximal), blue (pc = pericentromeric), green (d = distal) and turquoise (i = interstitial). 1/5 = fusion between autosome 1 with autosome 5, X4 = neo-X (X/4 fusion), X2 = neo-X (X/2 fusion), X4* = neo-X2, Y = neo-Y, Y2/4 = neo-Y (2/4 fusion). Chromosome locations for R. bergii were taken from Palacios-Gimenez et al. (2015).
Figure 2 in Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae)
Figure 2. Chromosome location of heterochromatic regions in eight Ronderosia species. White areas represent the absence of that chromosome. Coloured squares indicate a heterochromatic block revealed by C-banding in a particular location; light blue (pr = proximal), blue (pc = pericentromeric), green (d = distal) and turquoise (i = interstitial). ND indicates no C-banding data. CH = completely heterochromatic, 1/5 = fusion between autosome 1 with autosome 5, X4 = neo-X (X/4 fusion), X2 = neo-X (X/2 fusion), X4*=neo-X2, Y = neo-Y, Y2/4 = neo-Y (2/4 fusion). Heterochromatic block locations for R. bergii were taken from Palacios-Gimenez et al. (2015).
Data from: Chromosomes tell half of the story: the correlation between karyotype rearrangements and genetic diversity in sedges, a group with holocentric chromosomes
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Data from: Analysis of the karyotype structure in Ricolla quadrispinosa (Linneus, 1767): inferences about the chromosomal evolution of the tribes of Harpactorinae (Heteroptera, Reduviidae)
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Comparative karyotype analysis in chickpea (Cicer arietinum L.) using oligo painting FISH
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