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opennotspecifiedJul 2018View details →

FIGURE 4 in Description of the eggs of Psorophora ciliata and Psorophora ferox (Diptera: Culicidae, Aedini) from the east of the Brazilian state of Santa Catarina using scanning electron microscopy

FIGURE 4. Micropyle and tubercles of the egg of Psorophora ciliata (1200x).

opencc-zeroSep 2018View details →

Electron Microscopy

Reproduced

Figures 1-2 from: Brito P, Targueta C, Arruda W, Santos F, Bastos R (2019) The sexual dimorphic inguinal glands of the frog species Ololygon centralis (Anura: Hylidae) at light and transmission electron microscopy. Zoologia 36: 1-9. https://doi.org/10.3897/zoologia.36.e29356

Figures 1-2 Photographs of the lateral sides of a O.centralis male (1) and female (2). The dashed line marks the limit of the inguinal gland in males that are absent in females. Scale bars: 1 mm.

Figures 11-15 from: Brito P, Targueta C, Arruda W, Santos F, Bastos R (2019) The sexual dimorphic inguinal glands of the frog species Ololygon centralis (Anura: Hylidae) at light and transmission electron microscopy. Zoologia 36: 1-9. https://doi.org/10.3897/zoologia.36.e29356

Figures 11-15 Electron micrographs of the serous glands of the inguinal region. (11) Low magnification of the secretory syncytium with two visible nuclei (n) and also a sizeable cytoplasmic secretion aggregate (s). Notice the syncytium center (sc) filled with electron dense secretion and also the clear space (*) between syncytium basis and myoepithelial cells (m). Around the myoepithelial cells are some collagen fibrils (co). (12–13) Medium magnification of syncytium, where it is possible to notice some cytoplasmic secretion aggregate (s) and some regions of the cytoplasm with medium electron density (c). (14–15) Major magnifications of two large cytoplasmic secretion aggregate, with mixed portions of electron dense secretion (s) with medium electron density cytoplasm (c). (p) basal digitiform projections; (rer) rough endoplasmic reticulum. Sacale bars: 14, 15 = 1 μm, 12, 13 = 3 μm, 11 = 5 μm.

Figures 3-10 from: Brito P, Targueta C, Arruda W, Santos F, Bastos R (2019) The sexual dimorphic inguinal glands of the frog species Ololygon centralis (Anura: Hylidae) at light and transmission electron microscopy. Zoologia 36: 1-9. https://doi.org/10.3897/zoologia.36.e29356

Figures 3-10 Photomicrographs of histological sections of the male inguinal gland region of O.centralis. (3–6, 8) Histological sections stained with HE. 3) Section of skin from the peripherical region of the inguinal gland. Notice that only mucous glands are present. 4) Low magnification micrograph showing the presence of many syncytial glands (g), with arrows indicating the lateral limits of the inguinal gland. (4–6) Major magnifications of the glandular apical portion, with many melanocytes (m), mucous glands (mc) and myoepithelial cells (open arrows). Note the glandular ducts (dc). 7) Histological section submitted to PAS reaction. Notice that only some cells of the mucous glands (mc) exhibit a positive reaction (arrowheads). (8) Major magnification of the lateral base portion of the syncytium, with colloidal secretion (s) in syncytium cytoplasm. Note also a blood vessel in the connective tissue. (9) Methacrylate section treated with potassium permanganate and oxalic acid and stained with Nile blue. Notice the bleached melanocytes (m) and some syncytial cytoplasmic projections (*) through the glandular secretion (s). (10) Methacrylate section stained with toluidine blue. Notice the pale blue color of the secretion suggesting it is alkaline, contrasting with the dark blue color of the glandular syncytium (gs). (e) epidermis; (d) dermis; (black open arrow) myoepithelial cells; (c) blood cells. Scale bars: 5, 6, 8 = 10 μm, 3, 7, 9, 10 = 20 μm; 4 = 200 μm.

Figures 16-18 from: Brito P, Targueta C, Arruda W, Santos F, Bastos R (2019) The sexual dimorphic inguinal glands of the frog species Ololygon centralis (Anura: Hylidae) at light and transmission electron microscopy. Zoologia 36: 1-9. https://doi.org/10.3897/zoologia.36.e29356

Figures 16-18 (16) The basal portion of the syncytium with digitiform projections (p) and the clear space (*) between them and the myoepithelial cells. Notice the myoepithelial cells nuclei (n) and the collagen fibrils. (17) Detail of the connective tissue between two neighbor alveoli, with myoepithelial cells (m) and collagen fibrils (c). (18) The basal portion of a syncytium with intricate projection labyrinth. Notice the syncytium nucleus with irregular outline (n). Scale bars: 18 = 1 μm, 16, 17 = 3 μm.

opencc-zeroDec 2017View details →

Data from: Sperm head morphology is associated with sperm swimming speed: a comparative study of songbirds using electron microscopy

Sperm exhibit extraordinary levels of morphological diversification across the animal kingdom. In songbirds, sperm have a helically shaped head incorporating a distinct acrosomal membrane or 'helical keel', the form and extent of which varies across species. The functional significance of this helical shape, however, remains unknown. Using scanning electron microscopy, we quantified inter- and intra-specific variation in sperm head morphology across 36 songbird species (Passeriformes: Passerida). Using phylogenetic comparative methods, we investigated the relationship between sperm head morphology and both sperm swimming speed and the frequency of extra-pair young (EPY). We found that species whose sperm had a relatively more pronounced helical form (i.e. long acrosome, short nucleus, wide helical membrane, and a more pronounced waveform along the sperm head 'core') had faster-swimming sperm. We found no evidence of a relationship between inter-specific variation in sperm head morphology and EPY, although we did find that among- and within-male variation in sperm head traits were negatively correlated with EPY. Applying principles of fluid mechanics, we discuss how the helical form of the sperm head may influence swimming speed, and suggest that further studies considering aspects of sperm morphology beyond sperm length are needed to improve our understanding of sperm structure-function relationships.

opencc-zeroDec 2015View details →

Data from: Reconstruction of genetically identified neurons imaged by serial-section electron microscopy

Resolving patterns of synaptic connectivity in neural circuits currently requires serial section electron microscopy. However, complete circuit reconstruction is prohibitively slow and may not be necessary for many purposes such as comparing neuronal structure and connectivity among multiple animals. Here, we present an alternative strategy, targeted reconstruction of specific neuronal types. We used viral vectors to deliver peroxidase derivatives, which catalyze production of an electron-dense tracer, to genetically identified neurons, and developed a protocol that enhances the electron-density of the labeled cells and while retaining quality of the ultrastructure. The high contrast of the marked neurons enabled two innovations that dramatically speed data acquisition: targeted high-resolution reimaging of regions selected from rapidly-acquired lower resolution reconstruction, and an unsupervised segmentation algorithm. This pipeline reduces imaging and reconstruction times by at least two orders of magnitude, facilitating directed inquiry of circuit motifs.

opencc-by-4.0Aug 2021View details →

Figure 1 from: Diakova AV, Polilov AA (2021) Porous or non-porous? The challenge of studying unusual placoid sensilla of Megaphragma wasps (Hymenoptera, Trichogrammatidae) with electron microscopy. In: Proshchalykin MYu, Gokhman VE (Eds) Hymenoptera studies through space and time: A collection of papers dedicated to the 75th anniversary of Arkady S. Lelej. Journal of Hymenoptera Research 84: 69-73. https://doi.org/10.3897/jhr.84.68707

Figure 1 Structure and ultrastructure of unusual placoid sensella (PS) in female Megaphragma amalphitanumA–GSEMH 3D-reconstruction I, JFIB-SEMA third flagellomere bearing PSB, CPS wall sputtered with gold, sputtering thickness 20–25 nm D, EPS wall sputtered with chromium in ESEM mode F, GPS wall sputtered with gold, sputtering thickness 15 nm, orbital rotation with an inclination of up to 45° I cross section through the middle of PSJ cross section through the outgrowth of PSH three-dimensional reconstruction of PS and one of the sensory neurons. Abbreviations: cu – cuticle, de – dendrite, le – ledge, nb – neuron body. Arrows designate the locations of the pores.

opencc-by-4.0Dec 2022View details →

Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms

Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e).

opencc-zeroAug 2023View details →

Electron microscopy images of thalamus from acutely hydrogen sulfide poisoned mice

To date, the mitochondrial morphological changes that take place after an acute H2S exposure are not well described. In the present study, we analyzed images of transmission electron microscopy of thalami from mice subjected to a single acute (1000 ppm) H2S exposure and euthanized at various time points to assess the mitochondrial structure and morphometric parameters over time. From our observations, changes induced by H2S up to 48 h were mostly limited to increased cristae and matrix compartments' disorganization in mainly accumulated round-shaped mitochondria. At 72 h, H2S induced a spectrum of morphological cellular changes. Impaired H2S-mediated energy-producing capacity of mitochondria relative to their normal-sized counterparts was inferred from our observations of disorganization and paucity of the inner mitochondrial membrane, the infoldings of which—into cristae—are well-documented to increase the surface area for ATP production, the evaluation of a disorganized network, and likely a disrupted mitophagy because of changes in mitochondrial shape.