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1,204 results for “Enzymes”
Fig. 7 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 7. Mass spectra and retention index of the hydroxylated sesquiterpene generated by PmSTS mutants L454G and L454A. NIST library mass spectra of (A) sesquicineole, (B) cis-sesquisabinene hydrate, (C) trans-sesquisabinene hydrate, and (D) α-bisabolol are shown at bottom of each respective sample mass spectra.
Fig. 4 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 4. Structural analysis by circular dichroism (CD) of PmSTSΔ24WT and L454 mutants. Far-UV CD spectra (190–260 nm) of PmSTSΔ24 WT, PmSTSΔ24Y390S/L454G, PmSTSΔ24L454G and PmSTSΔ24L454A are shown. Buffer used was 20 mM Tris pH 8.0, 100 mM NaCl 2 mM βME. Protein concentration was 10 μM. Further secondary structure variations in percentages for each L454 mutant and WT were determined using the JASCO SSE program are presented in Table S2.
Fig. 3 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 3. Analysis of monomeric fraction of PmSTS Wild Type and mutant proteins using (A) 12% SDS PAGE and (B) 10% Native PAGE. The SDS PAGE showed high purity of PmSTS protein were obtained for wild type and for all mutants except for PmSTSΔ24W286A and PmSTSΔ24Y390S. The native PAGE showed several species of PmSTSΔ24WT, PmSTSΔ24V466E, and PmSTSΔ24Y390S/L454G that can be resolved on a 10% native gel. However, only one species of PmSTSΔ24L454G and PmSTSΔ24L454A was resolved on a 10% native gel. Smearing bands were observed for PmSTSΔ24W286A and PmSTSΔ24Y390S on the 10% native gel, probably due to the low purity as judged by 12% SDS PAGE.
Fig. 1 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 1. Superimposition of PmSTS homology model (green) with the crystal structure of Nicotiana tabacum 5-epi-aristolochene synthase (NtEAS, PDB ID:3M01). NtEAS has a FPP ligand analog (2-trans, 6-trans)-2- fluorofarnesyl diphosphate (FPF) bound in the active site (pink) shows an RMSD of 1.4 Å over 518 aligned Cα atoms. The Mg2+ ions in the active site of NtEAS are shown in yellow spheres. PmSTS and NtEAS share ~38% sequence identify. The putative active residues of PmSTS (W286, Y390, L454 and V466) shown heavier sticks were selected for mutagenesis studies. The structure superimposition was carried out using SSM in COOT (Emsley et al., 2010). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 2. Size exclusion chromatography (SEC) profiles of affinity chromatography purified wild-type and mutant proteins. The chromatography was performed on a HiLoad Superdex column 200 PG. Detection was at 280 nm. (A) PmSTSΔ24 WT, (B) PmSTSΔ24 V466E, (C) PmSTSΔ24 W286A, (D) PmSTSΔ24 Y390S, (E) PmSTSΔ24L454A, (F) PmSTSΔ24L454G, and (G) PmSTSΔ24Y390S/L454G were run over a size exclusion column to verify the oligomeric status of generated proteins. Elution profiles of β-sesquiphellandrene synthase proteins are represented together with protein standard: bovine thyroglobulin (670 kDa), bovine gamma globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), vitamin B12 (1.35 kDa). The peaks eluted at ~48 mL correspond to protein aggregated during the purification process, and peaks eluted at ~80 mL correspond to the monomeric size of PmSTSΔ24 protein (65 kDa).
A Customized Bayesian Algorithm to Optimize Enzyme-Catalyzed Reactions
<p>Data underlying the figures in the publication "A Customized Bayesian Algorithm to Optimize Enzyme-Catalyzed Reactions" published in <em>ACS Sustain. Chem. Eng.</em>, <strong>2023</strong>, <a href="https://doi.org/10.1021/acssuschemeng.3c02402">https://doi.org/10.1021/acssuschemeng.3c02402</a>.</p> <p>Table of contents:</p> <ul> <li><strong>sc3c02402_si_001.pdf</strong>, <strong>sc3c02402_si_002.pdf</strong>, <strong>sc3c02402_si_003.pdf, sc3c02402_si_004.pdf</strong>: DNA sequences, supplementary figures, materials, methods, availability of the program, synthesis protocols</li> <li><strong>sc3c02402_raw_data.zip</strong>: Raw data</li> </ul>
Cold-Active Enzyme from Glaciozyma antarctica PI12: In Silico Approach
<p>The input files, structures and datasets utilized to generate the computational results featured in the manuscript titled "Low Temperature Adaptation of Cold-Active Lipase from <em>Glaciozyma antarctica</em> PI12: Insights from Alphafold2 Prediction and Molecular Dynamics Simulation". For further elaboration and specific information, kindly refer to the publication. This research was made possible with the generous support of the Universiti Putra Malaysia.</p>
Dataset for manuscript "Turnover number predictions for kinetically uncharacterized enzymes using machine and deep learning"
<p>Dataset for the github repository containing the code for the manuscript "Turnover number predictions for kinetically uncharacterized enzymes using machine and deep learning".</p>
The Role of oAncreatic Enzyme ReplAcement Therapy (PERT) on Mucosal Visibility Before Upper GI Endoscopy
ClinicalTrials.gov study NCT06218537. IPD Sharing: NO. Countries: 1. Publications: 6.
Angiotensin Converting Enzyme Inhibitors & Contrast Induced Nephropathy in Patients Receiving a Cardiac Catheterization
ClinicalTrials.gov study NCT00317252. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Uptake of the Antifungal Miconazole and Effect on Estrogen Metabolizing Enzymes in Humans
ClinicalTrials.gov study NCT00668538. IPD Sharing: Not stated. Countries: 1. Publications: 1.
A Study to Evaluate ASP8232 as Add-On Therapy to Angiotensin Converting Enzyme Inhibitor (ACEi) or Angiotensin Receptor Blocker (ARB) in Reducing Albuminuria in Patients With Type 2 Diabetes and Chron
ClinicalTrials.gov study NCT02358096. IPD Sharing: YES. Countries: 9. Publications: 1.
Evaluation of Tranexamic Acid for Angiotensin-converting Enzyme Inhibitor-induced Angioedema in the Emergency Department
ClinicalTrials.gov study NCT06096077. IPD Sharing: YES. Countries: 1. Publications: 11.
The Effect of SOD Enzyme on Frailty and HRQOL Among Indonesian Pre-frail Elderly: A Double Blind Randomized Controlled Trial
ClinicalTrials.gov study NCT02753582. IPD Sharing: NO. Countries: 1. Publications: 17.
Exocrine Pancreatic Insufficiency After Acute Pancreatitis and Pancreatic Enzyme Replacement Therapy
ClinicalTrials.gov study NCT05480241. IPD Sharing: NO. Countries: 1. Publications: 28.
Arterial Stiffness and General Anesthesia Induced Hypotension in Patients on Angiotensin-converting Enzyme Inhibitors
ClinicalTrials.gov study NCT02416024. IPD Sharing: Not stated. Countries: 1. Publications: 7.
Effect of Sinbiotic With Multispecies Probiotics on Liver Parameters Liver Enzymes, Ultrasound, Elastography and Adipokines in Same Cases) in Patients With Metabolic Associated Steatotic Liver Disease
ClinicalTrials.gov study NCT07025980. IPD Sharing: NO. Countries: 1. Publications: 12.
Study to Investigate the Mechanism of Action of an Oral Enzyme Treatment With Bromelain, Trypsin and Rutoside Versus Placebo in Subjects With OsTeoarthritis
ClinicalTrials.gov study NCT05038410. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Recombinant Human Angiotensin-converting Enzyme 2 (rhACE2) as a Treatment for Patients With COVID-19
ClinicalTrials.gov study NCT04287686. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Use of Ambulatory Blood Pressure Monitors to Assess Angiotensin Converting Enzyme Inhibitors
ClinicalTrials.gov study NCT02623036. IPD Sharing: Not stated. Countries: 1. Publications: 3.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.