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Monitoring of nutrient and sulfide concentrations in porewaters of mangrove forests from the Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, December 2000 - ongoing
To monitor soil chemistry in the mangrove sites SRS4, SRS5, SRS6, and SRS7, and TS/Ph6b, TS/Ph7b and TS/Ph8, porewater concentrations of sulfide, PO4, NH4, NO2 and NO3 have been analyzed. See also related porewater-physical-characteristics data package knb-lter-fce.1169 (https://portal.edirepository.org/nis/mapbrowse?scope=knb-lter-fce&identifier=1169).
Relative Abundance of Soft Algae From the Comprehensive Everglades Restoration Plan (CERP) Study (FCE), Florida, USA, September 2005 to November 2011
Relative soft algae data collected between September 2005 and November 2011 "The Comprehensive Everglades Restoration Plan (CERP) focuses on “getting the water right” in the south Florida ecosystem—getting the right amount of water of the right quality to the right places at the right time" (USACE & DoI, 2015. Central and Southern Florida Project Comprehensive Everglades Restoration Plan) in the Everglades ecosystems. To inform CERP, since February 2005 we have been investigating the spatio-temporal variations of distribution, biomass and diversity of algae (key aquatic primary producers) in periphyton mats in relation to hydrology, nutrients and pH, and other environmental conditions.
Periphyton, hydrological and environmental data in a coastal freshwater wetland (FCE), Florida Everglades National Park, USA (2014-2015)
The characteristic, calcareous periphyton mats of the Everglades, and particularly their diatom assemblages, provide an ideal community to study the patterns and mechanisms of community assembly along environmental gradients with ecotones. Understanding patterns and mechanisms of diatom community assembly along salinity and P gradients can be incorporated into tools for predicting changes in these gradients, and the location and movement of the "white zone" ecotone, caused by saltwater intrusion and water management outcomes in the Southern Everglades. Patterns of environmental variation and periphytic-diatom community structure along the freshwater-marine gradient of Everglades National Park, FL., USA were examined by sampling along a series of 7 transects extending from oligotrophic, freshwater marshes through the ecotone and down to the northern edge of the fringing mangrove forests. Seven transects spanning the west-east extent of the southeast Everglades, from the Main Park Road in the west to the Model Lands in the east, were sampled once in the dry season (May) and once in the wet season (November) of 2014 and 2015. These data are published in "Mazzei and Gaiser. 2018. Diatoms as tools for inferring ecotone boundaries in a coastal freshwater wetland threatened by saltwater intrusion. Ecological Indicators. 88:190-204."
Biogeochemical data collected from Northeast Shark River Slough, Everglades National Park, Florida, USA, September 2006 - April 2025
This project was established in 2006 to document the pattern of abundance of key ecological indicators (e.g., surface water, soil, floc, periphyton and sawgrass) across the NESRS landscape. A total of 30 sites were established and monitored in 2006, 2007 and 2008. After the completion of 1-mile bridge in 2012, additional 10 new sites were established to observe the ecological impact of 1-mile bridge (known as Bridge & Census sites). In 2015, additional 40 sites were established along eight transects (T1-T8, known as near canal sites) in ENP marshes starting at, and roughly perpendicular to the L-29 canal. The purpose of these sites was to monitor the potential effects of Modified Water Deliveries (MWD) operations on changing nutrient concentrations and ratios in key ecological compartments due to increased downstream discharges from the L-29 canal beneath the 1-mile and 2.6-mile bridges and culverts along Tamiami Trail. Data collection is complete.
FCE LTER Taylor Slough/Panhandle-7 Site Scrub Red Mangrove (Rhizophora mangle) Leaf Gas Exchange Data, Florida, USA from January-December 2019
Rates of leaf gas exchange were measured monthly during the 2019 calendar year in a scrub Red mangrove (Rhizophora mangle (L.) L.) forest site (TS/Ph-7) near the mouth of Taylor River in southeastern Florida Everglades. Sampling of green mature leaves was designed to target scrub mangrove tree branches growing on slightly higher elevation mangrove island centers versus permanently inundated island edge habitats. Concurrent measurements of water depth and surface and porewater salinity were collected at each of the mangrove island habitats, with the research objective of assessing the effect of physicochemical variables on rates of leaf gas exchange (i.e., assimilation and stomatal conductance). Leaf gas exchange data were collected using the Li-6800 portable photosynthesis system (Li-COR, Lincoln, NE). Additional data on leaf functional traits and nutrient concentrations and environmental data from the site are included. Data are presented in five datasets (.csv).
Biogeochemical data collected in the eastern marl prairies of the Everglades (Florida, USA) before and after fire, between the years 2018 and 2021
The data included in this database were collected within a project funded by Everglades National Park (ENP). The project was aimed at understanding how fire, characterized by different intensities, differentially affects nutrient cycling in oligotrophic wetlands with distinct levels of phosphorus limitation. The data were collected in the eastern part of the Everglades, close to the boundary of ENP, in South Florida (Homestead). This part of ENP is characterized by marl soils, which are shallow (often less than 10 cm) and C-poor. The data were collected at two sites: one site was more oligotrophic (LP), while the other one had a certain degree of phosphorus enrichment (HP). The database includes date and location (i.e. site and plot) of each sampling event, surface water physio-chemistry, vegetation composition and structure, including dominant macrophyte estimated biomass, litter and root decomposition rates, and carbon, nitrogen, and phosphorus concentrations in the soil, flocculent detrital material, periphyton, sawgrass leaves and roots, and the water column. All these data were collected at different times between September 2018 and March 2021, before and after a prescribed fire that was carried out in February 2020 and which burned all the plots. Each row within the database identifies a unique combination of date and sampling plot.
Monthly monitoring fluorescence data for Shark River Slough and Taylor Slough, Everglades National Park, Florida, USA (FCE LTER) for 2012 - 2020
Dissolved organic matter plays an important role in biogeochemical processes in aquatic environments such as elemental cycling, microbial loop energetics, and the transport of materials across landscapes. Since most of N (> 90%) and P (around 90%) is in the organic form in the oligotrophic subtropical Florida Coastal Everglades (FCE), study of the source and dynamics of dissolved organic matter (DOM) in the ecosystem is crucial for the better understanding of the biogeochemical cycling of nutrients. FCE are composed of estuaries with distinct regions with different biogeochemical processes. Freshwater marsh primarily receives terrestrial input and local autochthonous vegetation production. Mangrove ecotone, nevertheless, is affected by the tidal contributions from Florida Bay and local mangrove production. Florida Bay (FB) is a wedge-shaped shallow oligotrophic estuary which lays south of the Everglades, the bottom of which is covered with a dense biomass of seagrass. The sources of both freshwater and nutrients in FCE are difficult to quantify, owing to the non-point source nature of runoff from the Everglades and the dendritic cross channels in the mangroves. Furthermore, the combination of multiple DOM sources (freshwater marsh vegetation, mangroves, phytoplankton, seagrass, etc.), and the potential seasonal variability of their relative contribution, along with the history of (photo)chemical and microbial diagenetic processing, and complex advective circulation, makes the study of DOM dynamics in FCE particularly difficult using standard schemes of estuarine ecology. Quantitative information of DOM is very useful to investigate the biogeochemical cycling of DOM to a certain degree, however, qualitative information is necessary to better understand the source and dynamics of DOM. Since fluorescence spectroscopic techniques are very sensitive, quick and simple, they have been applied to investigate the fate of DOM in estuaries.Monthly fluorescence monitoring data
Inventory of High-resolution phylogenetic profiles of the planktonic microbial communities (via 16S and 18S rRNA gene amplicons) from Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, 2017 - ongoing
Planktonic microbial communities mediate many vital biogeochemical processes in wetland ecosystems, yet compared to other aquatic ecosystems, like oceans, lakes, rivers, or estuaries, they remain relatively underexplored. Our study site, the Florida Everglades (USA)—a vast iconic wetland consisting of a slow-moving system of shallow rivers connecting freshwater marshes with coastal mangrove forests and seagrass meadows—is a highly threatened model ecosystem for studying salinity and nutrient gradients, as well as the effects of sea level rise and saltwater intrusion. This dataset provides the first high-resolution phylogenetic profiles of planktonic bacterial and eukaryotic microbial communities (using 16S and 18S rRNA gene amplicons) from these environments. The dataset contains 16S and 18S rRNA data from 2017, and contains 16S rRNA data for monthly (2019) and quarterly water samples (2020-ongoing). The 2017 data are published in Laas et al. 2022. A detailed list of sequence data and their accession numbers in GenBank is provided and will be updated as more data are published. This data package is an inventory of sequence read archive (SRA) entries available through GenBank BioProject PRJNA525456 (at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA525456) and BioProject PRJNA1018945 (at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1018945). This data package is associated with the following publication: Laas, P., Ugarelli, K., Travieso, R., Stumpf, S., Gaiser, E. E., Kominoski, J. S., & Stingl, U. (2022). Water column microbial communities vary along salinity gradients in the Florida Coastal Everglades wetlands. Microorganisms, 10(2), 215. https://doi.org/10.3390/microorganisms10020215 Instead of citing this package, which is an inventory, please cite the original GenBank data or journal article, as appropriate. Citation guidance for the journal article is available on the respective publisher's website.
Field survey and long-term measurements of biological, geomorphic, and hydrologic properties across ecosystem states in a non-tidal, salinizing peat marsh in Everglades National Park, Florida, USA, June 2018 - ongoing
This dataset package details a field survey and long-term measurements of biological, geomorphic, and hydrologic properties across ecosystem states found within a salinizing, non-tidal peat marsh in the coastal Everglades. There are four datasets included: FCE1250_Lamb_AltStableState_HydroGeo includes point measurements of soil surface elevation, bedrock elevation, soil depth, water depth, and porewater salinity across ecosystem states: emergent marsh (sawgrass dominated), submerged marsh (submerged aquatic vegetation), and unvegetated open water. The survey was conducted between November 2019 to January 2020. FCE1250_Lamb_AltStableState_Bio contains plot-scale (1 m^2) biological measurements from emergent marsh (sawgrass). Biological measurements (total sawgrass, average individual sawgrass biomass, and total sawgrass aboveground biomass), are inherently plot-level but geomorphic and hydrologic measurements were averaged (n = 3) for each plot. Standard deviations are provided. FCE1250_Lamb_AltStableState_Bio_LongTerm contains biological plot-scale (1 m^2) measurements at a bi-monthly timestep from Nov 2019 - July 2022 from a subset of plots (n = 9) included in the FCE_1250_Lamb_AltStableState_Bio dataset. Each bi-monthly measurement is the average value across all 9 nine plots. Standard deviations are also included. FCE_1250_Lamb_AltStableState_SETMH contains long-term measurements of soil surface elevation change and vertical accretion from surface elevation change and marker horizon plots from June 2018 - May 2023. Average and standard errors across all plots are included as well as measurements from each plot. Also included is the cumulative number of dry days based on the average soil surface elevation for all plots. Data collection for all datasets is complete, except for FCE_1250_Lamb_AltStableState_SETMH. SET-MH measurements are still ongoing.
Groundwater and surface water chemistry from the Hole-in-the-Donut and nearby Taylor Slough in Everglades National Park, Florida, USA: 2015-2016
This research investigated the effects of the vegetation and soil removal on the hydrologic conditions of the Hole-in-the-donut (HID) of Everglades National Park. Groundwater and surface water samples were collected from 2 wells within the HID (DO1,DO3) and two wells outside the HID in adjacent Taylor Slough (NP-67, TSB). Surface water was collected at each site if present.
Alligator pond food-web sampling in Shark River Slough and Taylor Slough, Everglades National Park, Florida, USA, 2018–2019
These datasets were used to investigate if American Alligators engineer differences in nutrient availability and changes to community structure by their creation of “alligator ponds” compared to the surrounding phosphorus (P)-limited oligotrophic marsh in the Everglades. We used a halo sampling design of three distinct habitats extending outward from ten active alligator ponds across a hydrological gradient. We performed nutrient analysis on basal food-web resources and quantitative community analyses, and stoichiometric analyses on plants and animals. These data underly the work in Strickland et al. (2023). An apex predator engineers wetland food-web heterogeneity through nutrient enrichment and habitat modification. Journal of Animal Ecology.
Long-Term Aquatic Faunal Sampling via Throw Trap in Shark River Slough, Everglades, Florida, USA, 1978 – 2021
Long-term monitoring of fish communities in the Everglades using 1-m2 throw traps started in 1978 and is ongoing. Data represent the number of individuals per species collected per throw trap (i.e., individuals per square meter). From 1978-1995 sampling occurred five times per year, referred to as periods, at one plot per site and the number of throws was determined by estimates of inter-sample variance. In 1996, the spatial extent of monitoring was expanded to include additional sites and multiple plots per site. At each plot 5-7 throw trap samples were collected with sampling location within a plot being drawn from a random number table. Days since dry (DSD) are calculated as the number of days since depth was last < 5 cm using the Everglades Depth Estimation Network and in situ depth measurements. Statistical comparisons of community composition can be found in FCE1258_community_change.Rmd
Stomach contents and stable isotopes from the aquatic food web in Everglades National Park, Florida, USA, 2018-2019
Stable isotope samples were collected among habitats and between seasons at multiple sites in both Shark River Slough and Taylor Slough of Everglades National Park to represent as much of the aquatic food web as possible. Stomach contents were also recorded for many of the collected vertebrate samples. Sampling occurred between October 2018 and April 2019. These data are a follow up study to the FCE1263 data package (https://portal.edirepository.org/nis/mapbrowse?scope=knb-lter-fce&identifier=1263) following the invasion of several fishes, most notably the African Jewelfish (Rubricatochromis letourneuxi), although there are some differences in habitats sampled and overall spatial coverage. R code for analyses associated with these data are available at https://github.com/pjflood/diet_and_pond_enrichment. Data collection is complete.
Stomach contents (1977-1981) and stable isotopes (1994) from the Everglades, Florida, USA from the publication "Fishes in a seasonally pulsed wetland show spatiotemporal shifts in diet and trophic niche but not shifts in trophic position"
Stomach contents of fishes (1977-1981) and stable isotopes of fishes, invertebrates, and basal resources (1994) were collected from spikerush marsh, sawgrass ridge, and alligator pond habitats in Shark River Slough, Everglades National Park, Florida, USA. These data were used to quantify diet, trophic niche area, trophic position, basal resource use and how these metrics vary among size classes, seasons, and habitats. Data collection is complete. These data support Flood et al. (2023). Associated R code will be made available through Peter Flood's GitHub: https://github.com/pjflood/historic_everglades_aquatic_food_web. References: Flood, Peter J., William F. Loftus, and Joel C. Trexler. "Fishes in a seasonally pulsed wetland show spatiotemporal shifts in diet and trophic niche but not shifts in trophic position." Food Webs 34 (2023): e00265. https://doi.org/10.1016/j.fooweb.2022.e00265
Mangrove Leaf Litter Carbon and Nutrients from the Shark River Slough, Everglades National Park (FCE), South Florida, USA, January 2019 - ongoing
Mangrove litterfall dynamics have been monitored in all Shark River sites (SRS-4, SRS-5, SRS-6) since January 2001 using the same collection method stated in Castañeda-Moya et al. 2013 (metadata: knb-lter-fce.1195) and Danielson et al. 2017. Briefly, litterfall was collected monthly at all sites (10 baskets per site) using permanent 0.25 m2 wooden baskets supported approximately 1.3 m above the soil surface and lined with 1 mm mesh screening. Litterfall from each basket was sorted, dried, and weighed by leaf species, reproductive parts by species, and woody material. Leaf litter data from different years (2019, 2020, 2021, 2023) were selected for each site to identify species-specific foliar carbon and nutrient (N and P) content. Monthly leaf litter samples were analyzed separately by species for all years after grinding with a Wiley Mill to pass through a 40-µm mesh screen. Total leaf litter C and N contents were determined with a Carlo-Erba NA-1500 elemental analyzer (Fisons Instruments Inc., Danvers, MA, USA). Total leaf litter P was extracted using an acid-digest (HCl) extraction, and concentrations of SRP were determined by spectrophotometric analysis (Methods 365.4 and 365.2, USA EPA 1983). Litterfall data collection is ongoing every year since 2001, while C and nutrients analyses are performed every other year after 2021. See also Shark River mangrove litterfall data (knb-lter-fce.1195) on the FCE LTER website's data catalog or in the EDI repository (https://portal.edirepository.org/nis/mapbrowse?scope=knb-lter-fce&identifier=1195). References: Castañeda-Moya, E., Twilley, R. R., & Rivera-Monroy, V. H. (2013). Allocation of biomass and net primary productivity of mangrove forests along environmental gradients in the Florida Coastal Everglades, USA. Forest Ecology and Management, 307, 226-241. Danielson, T.M., V.H. Rivera-Monroy, E. Castaneda-Moya, H. Briceno, R. Travieso, B.D. Marx, E. Gaiser, and L.M. Farfan. 2017. Assessment of Everglades mangrove forest re
Field survey of mangrove regeneration, porewater variables, and light in mangrove forests in Everglades National Park, Florida, USA, July 2020 - August 2022
This dataset package encompasses measurements from field surveys of mangrove regeneration, porewater variables, and light conditions across six mangrove sites in the coastal Everglades. The goal of this project was to quantify mangrove regeneration of seedlings and saplings in mid- and downstream locations within three estuaries in Everglades National Park, Florida, USA. We assessed the effects of porewater variables and light conditions on the observed regeneration patterns. The package includes seven datasets: FCE1268_Porewater: Contains measurements of porewater salinity, sulfide, ammonia, nitrite, orthophosphate, and nitrate at a 30 cm depth. Porewater surveys were conducted biannually from 09-10-2020 to 05-17-2022. See also similar porewater data for Florida Coastal Everglades (FCE) long-term sites in data packages knb-lter-fce.1169 and knb-lter-fce.1171, which contain data for SRS-5 and SRS-6, available in the FCE LTER website's data catalog or the EDI repository. FCE1268_Foliar_Nutrient_Content dataset, collected in August 2022, includes measurements of foliar nutrient content (total carbon, total nitrogen, and total phosphorus) for three mangrove species (A. germinans, L. racemosa, R. mangle) of two life stages—seedlings (height < 1 m) and saplings (height ≥ 1 m and Diameter at Breast Height (DBH) < 2.5 cm). FCE1268_Light contains light intensity (foot-candle) measurements taken at 1-hour intervals from 09-18-2020 to 08-29-2022 at mangrove sites and converted photosynthetic active radiation values from an outdoor mesocosm experiment. FCE1268_Sapling_Density provides biannual count measurements of individuals at the sapling plot level (4 m^-2) within each site from 07-09-2020 to 08-29-2022. FCE1268_Seedling_Density contains biannual count measurements of individuals at the seedling plot level (m^-2) within each site from 07-07-2020 to 08-29-2022. FCE1268_Sapling_Regeneration contains height, crown area, and stem elongation measurements of tagged sapling indiv
Root productivity of riverine and scrub mangroves from the Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, March 2024 - April 2024
Root productivity of riverine and scrub mangroves in the Florida Everglades: Mangrove root productivity in the shallow root zone (0-45 cm depth) was estimated at all Shark River (SRS-4, SRS-5, SRS-6, SRS-7) and Taylor River (TS/Ph-6, TS/Ph-7) sites in March-April 2024. Root productivity was estimated with the ingrowth core technique using the same sampling protocol previously published for the study area (Castañeda-Moya et al. 2011). Ingrowth cores (10.2 cm diameter x 45 cm length) were made of synthetic material (3 mm mesh) and filled with root-free commercial sphagnum peat moss. This material has similar soil properties (i.e., bulk density, organic matter content, total C and N) as mangrove peat in our study sites as previously reported by Castañeda-Moya et al. (2011). Ingrowth cores were installed in holes made out with a PVC coring device (10.2 cm diameter x 45 cm length). At each site, 8 ingrowth cores were deployed vertically into the soil to a depth of 45 cm and retrieved one year later (March-April 2024). After collection, ingrowth cores were processed individually and initially rinsed with water through a 1-mm screen mesh to remove soil particles and peat moss material. Live roots were separated manually based on their buoyancy, turgor, and color (Castañeda-Moya et al. 2011; Cormier et al. 2015; Medina-Calderon et al. 2021). Live roots were further sorted into three size diameter classes including fine (<2 mm), small (2-5 mm), and coarse (5-20 mm). Roots greater than 20 mm in diameter were not included in this study due to sampling limitations (i.e., core area). All root samples were oven-dried at 60°C to a constant mass and weighed. Root growth within each ingrowth core following one year of incubation was used to estimate annual root productivity (g m⁻² yr⁻¹) in the shallow root zone across all mangrove sites. All data collection and processing were funded by FCE-LTER. Data collection is complete. References: Castañeda-Moya, E., R.R. Twilley, V.H. Rivera-
Environmental and periphyton composition data from Biscayne Bay Coastal Wetlands, Florida, USA, July 2022 - November 2022
Environmental and periphyton data were collected from transects in the Biscayne Bay Coastal Wetlands (BBCW) during the wet and dry seasons of 2022 to investigate the rate of carbonate sediment production by periphyton. Environmental data include surface water metrics (pH, salinity, conductivity, and water depth) and soil depths. Periphyton data include nutrient, production, and diatom species composition in samples collected from artificial substrates (periphytometers) placed in the field. Data collection for this project is complete, although the South Florida Management District continues to monitor these transects for a larger ongoing BBCW project.
Scrub Mangrove Annual Leaf Net Primary Production Data in Taylor River Slough, Florida Everglades, Florida, USA (FCE LTER): 2012-2021
Foliar Net Primary Productivity (NPPF) of monospecific Rhizophora mangle scrub mangroves (tree height <2.5m) was assessed using modified leaf-tagging techniques (direct count, ring tracking) in the lower Everglades Taylor Slough (FCE-LTER sites: TS/Ph6b and TS/Ph7b). Rings made with plastic zip ties were used to mark and track new and old leaves twice yearly (dry vs. wet season). During each sampling period, total old and new leaves were counted in previously selected branches in vegetated areas inside 20 x 20 m plots (replicates) in each site. Data collection is complete. This data set is analyzed and discussed in the publication Rivera-Monroy et al. Linking Scrub Mangroves Long-Term (2012-2021) Foliar Net Primary Productivity and Spatial Distribution in the Everglades (Florida, USA): A Leaf Tagging Approach
Diatom composition and environmental data from the Greater Everglades, Florida, USA (2013-2020)
Environmental and diatom data were collected from sites in the Big Cypress National Preserve (BICY) by the South Florida/Caribbean Inventory and Monitoring Network of the National Park Service and from sites in the Everglades Protection Area (EPA) as part of the Monitoring and Assessment Program of the Comprehensive Everglades Restoration Plan. Samples from years 2012, 2013, 2019, 2019, and 2020 are included in this dataset. Environmental data include drier variables that have been found to influence diatom assemblage composition in the greater Everglades ecosystem, including periphyton mat total phosphorus (a proxy for phosphorus in the environment), water column pH, water column conductivity, water depth, days since last dry, and hydroperiod. Diatom data include diatom species composition as percent relative abundances. Code included is pertinent to the methods described in "Robust species optima estimates from non-uniformly sampled environmental gradients" by Solomon et al. 2025, Journal of Paleolimnology.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.