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573 results for “immune function”
THE INTEGRATIVE GENOMIC AND FUNCTIONAL IMMUNOLOGICAL ANALYSES OF COLORECTAL CANCER INITIATING CELLS TO MODULATE STEMNESS PROPERTIES AND THE SUSCEPTIBILIY TO IMMUNE RESPONSES [miRNA]
GEO Series GSE287585. Homo sapiens. 23 samples. Type: Non-coding RNA profiling by array.
Spatial immune profiling defines a subset of human gliomas with functional tertiary lymphoid structures [GEOMX]
GEO Series GSE271255. Homo sapiens. 81 samples. Type: Other.
Single-cell profiling reveals altered immune landscape and impaired NK cell function in gastric cancer liver metastasis
GEO Series GSE246662. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function
GEO Series GSE36890. Mus musculus. 68 samples. Type: Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Background: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays. We tested the effect of multiple (1, 2, 3, 4, 5, 10) freeze-thaw cycles on the results of four commonly used immunological assays: haemolysis-haemagglutination titres, haptoglobin concentration, bacterial killing capacity and total immunoglobulins (IgY). We tested five different bird species from four different bird orders (Passeriformes, Columbiformes, Charadriiformes and Galliformes), and we included both captive and free-living individuals. In addition, we tested for haptoglobin concentrations and the haemolysis-haemagglutination assay if re-analysing samples 1 year apart led to different results. For the haemolysis-haemagglutination assay we also tested two different sources of rabbit blood, and we compared untreated microtitre plates with plates that were "blocked" to prevent nonspecific interactions between the plate and assay reagents. Results: Repeated freezing and thawing of plasma had no effect on lysis titres, haptoglobin concentrations, bacterial killing capacity, or total immunoglobulin levels. Agglutination titres were unaffected by up to five cycles but were lower after ten freeze-thaw cycles. For the haemolysis-haemagglutination assay and haptoglobin concentrations, re-analysing samples 1 year apart yielded highly correlated data. For the haemolysis haemagglutination assay,thesourceofrabbit blood did not influence the results, and the untreated vs. blocked plates differed slightly overall, but at the individual level assay results were highly correlated. Using different rabbit blood sources or different types of microtitre plates yielded highly correlated data.Conclusions: Our data suggest that repeated freeze-thaw cycles do not impair assay results to the point of influencing ecological or evolutionary conclusions. Plasma samples can be safely stored in one tube and thawed repeatedly for different assays. Nevertheless, we recommend consistent treatment of samples in terms of freeze-thaw cycles or other laboratory treatments to minimize the potential for introducing a systematic bias.
Potent anti-tumor immunity and reversal of CD8 T cell exhaustion by spatially and functionally targeting Treg cells in the tumor microenvironment
<p>1×10<sup>6</sup> of splenic GFP<sup>+</sup> Tregs were purified from WT (n=4) and KO mice (n=4) via FACS isolation. RNA was extracted using RNeasy Micro Kit (Qiagen) following its standard protocol and RNA degradation and contamination was monitored on 1% agarose gels. For RNA sequencing, libraries were prepared using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. All samples were prepared at the same time and sequenced on the same lane. Original image data file from high-throughput sequencing platforms (like Illumina) is transformed to sequenced reads (called Raw Data or Raw Reads) by CASAVA base recognition (Base Calling). Raw data in FASTQ format were processed through fastp. In this step, clean data (clean reads) were obtained by removing reads containing adapter and poly-N sequences and reads with low quality from raw data. All the downstream analyses were based on the clean data with high quality. Reference mouse genome and gene model annotation files were downloaded from genome website browser (NCBI/UCSC/Ensembl). Paired-end clean reads were mapped against the reference genome using the Spliced Transcripts Alignment to a Reference (STAR) software. FeatureCounts was used to count the read numbers mapped of each gene. And then RPKM (Reads Per Kilobase of exon model per Million mapped reads) of each gene was calculated based on the length of the gene and reads count mapped to this gene. The FPKM gene expression matrix, R code, DEG list, and targeted gene list are uploaded in this project.</p>
GCWB1176 Evaluates the Effectiveness and Safety of Immune Function
ClinicalTrials.gov study NCT05632341. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Assessment of Vitamin D Supplementation and Immune Function
ClinicalTrials.gov study NCT01399151. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Soy Bread Diet in Improving Immune Function in Participants With Prostate Cancer
ClinicalTrials.gov study NCT03654638. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Immune and Endocrine Function in Post-Traumatic Stress Disorder
ClinicalTrials.gov study NCT00100490. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Perioperative Immune Function in Patients Undergoing Colorectal Cancer Surgery
ClinicalTrials.gov study NCT04127864. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Bedside Assessment of Immune Function in Patients With Covid-19
ClinicalTrials.gov study NCT06879548. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Effects of Propofol-dexmedetomidine on Immune Function in Patients With Cancer Larynx
ClinicalTrials.gov study NCT02739958. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Natural Killer Cells Functions, Tumoral Escape to Immune System and Regulation of Natural Cytotoxixity Receptors in Haematological Malignancies
ClinicalTrials.gov study NCT01915992. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Study on Correlated Factors of Barrier and Immune Function in Stress Related Mucosal Disease (SRMD)
ClinicalTrials.gov study NCT03200158. IPD Sharing: NO. Countries: 1. Publications: 0.
Study of Plasma Tumor-promoting Factors and Immune Function After Laparotomy, Cytoreductive Surgery and HIPEC vs. PIPAC in Patients With Peritoneal Metastasis
ClinicalTrials.gov study NCT04122885. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Prebiotics and Immune Function in Middle Aged Humans
ClinicalTrials.gov study NCT00898599. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Study of the Effects of Time-Restricted Feeding on the Immune Function of Individuals With Obesity
ClinicalTrials.gov study NCT06899997. IPD Sharing: YES. Countries: 1. Publications: 0.
Ability of Beta-glucan Supplementation to Augment Immune Function
ClinicalTrials.gov study NCT03769012. IPD Sharing: NO. Countries: 1. Publications: 0.
Effects of Omega-3 Fish Oil Fat Emulsion on Rehabilitation and Immune Function After Radical Surgery Combined With Intraperitoneal Hyperthermic Infusion Chemotherapy in Patients With Locally Advanced
ClinicalTrials.gov study NCT06047158. IPD Sharing: Not stated. Countries: 1. Publications: 0.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.