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opencc-zeroDec 2021View details →

Rapid evolutionary divergence of a songbird population following recent colonization of an urban area

Colonization of a novel environment by a small group of individuals can lead to rapid evolutionary change, yet evidence of the relative contributions of neutral and selective factors in promoting divergence during the early stages of colonization remain scarce. Here, we use genome-wide SNP data to test the role of neutral and selective forces in driving the divergence of a unique urban population of the Oregon junco (Junco hyemalis oreganus), which became established on the campus of the University of California at San Diego (UCSD) in the early 1980s. Previous studies based on microsatellite loci documented significant genetic differentiation of the urban population as well as divergence in sexual signaling and life-history traits relative to nearby montane populations. However, the geographic origin of the colonization and the factors involved in the onset of the differentiation process remained uncertain. Our genome-wide SNP dataset confirmed the marked genetic differentiation of the UCSD population, and phylogenomic analysis identified the coastal subspecies pinosus from central California as its sister group instead of the neighboring mountain population. Demographic inference based on site frequency spectra recovered a time of separation from pinosus as recent as 20 to 32 generations, and a strong bottleneck at the time of colonization, suggesting a relevant role of founder effects and drift in the genetic differentiation of the UCSD population. However, we also found significant associations between environmental parameters characterizing the urban habitat of UCSD and genome-wide variants linked to functional genes. Some of the identified gene functions, like heavy metal detoxification and high-pitched hearing, have been reported as potentially adaptive in birds inhabiting urban environments. These results suggest that the interplay between founder events and directional selection may result in rapid shifts in both neutral and adaptive loci across the genome, and reveal the UCSD population of juncos as an ongoing case of divergence following the colonization of an anthropic environment.

opencc-by-4.0Apr 2022View details →

Fig. 5 in Landuse Patterns, Air Quality And Bird Diversity In Urban Landscapes Of Delhi

Fig. 5. CCA plot showing the relationships of landuse patterns, air pollutants and bird species abundance.

Fig. 2 in Variation In Blackbird, Turdus Merula (Passeriformes, Turdidae), Nest Characteristics In Urban And Suburban Localities In Crimea

Fig. 2. The differences in external depth between Blackbird nests in the park (N = 15) and forest (N = 10)

Fig. 4 in Variation In Blackbird, Turdus Merula (Passeriformes, Turdidae), Nest Characteristics In Urban And Suburban Localities In Crimea

Fig. 4. Number of component types in each nest layer: A — Outer layer, B — Medium layer, C — inner layer. Conclusion

Fig. 3 in Variation In Blackbird, Turdus Merula (Passeriformes, Turdidae), Nest Characteristics In Urban And Suburban Localities In Crimea

Fig. 3. The differences in external depth between Blackbird nest in tree (N = 12) and shrubs (N = 15).

Fig. 2 in Ecological And Faunistic Review Of The True Bugs Of Infraorder Cimicomorpha (Heteroptera) Of Urban Cenoses Of Kharkiv City (Ukraine)

Fig. 2. Ratio between main trophic groups of true bugs in terms of number of species and relative abundance (% out of the total number of true bugs). Names of groups in acronyms are the same as in the table 1.

Fig. 3 in Ecological And Faunistic Review Of The True Bugs Of Infraorder Cimicomorpha (Heteroptera) Of Urban Cenoses Of Kharkiv City (Ukraine)

Fig. 3. Ratio between hygropreference of main groups of true bugs in terms of number of species and relative abundance (% out of the total number of true bugs). Names of groups in acronyms are the same as in the table 1.

Fig. 1 in Ecological And Faunistic Review Of The True Bugs Of Infraorder Cimicomorpha (Heteroptera) Of Urban Cenoses Of Kharkiv City (Ukraine)

Fig. 1. Ratio between main biotopic groups of true bugs in terms of number of species and relative abundance (% out of the total number of true bugs). Names of groups in acronyms are the same as in the table 1.

Fig. 12 in Breeding Bird Assemblage In A Mosaic Of Urbanized Habitats In A Cenral European City

Fig. 12. Distribution of occupied territories of Cuculus canorus, Oriolus oriolus, Phasianus colchicus and Dendrocopos major.

Fig. 7 in Breeding Bird Assemblage In A Mosaic Of Urbanized Habitats In A Cenral European City

Fig. 7. Distribution of occupied territories of Acrocephalus plustris, A. Arundinaceus and A. scirpaceus.

Fig. 4 in Breeding Bird Assemblage In A Mosaic Of Urbanized Habitats In A Cenral European City

Fig. 4. Distribution of occupied territories of Serinus Fig. 5. Distribution of occupied territories of serinus, Phylloscopus collybita, P. trochilus and Phoenicurus ochruros. P. sibilatrix.

Fig. 2 in Breeding Bird Assemblage In A Mosaic Of Urbanized Habitats In A Cenral European City

Fig. 2. Distribution of occupied territories of Sylvia Fig. 3. Distribution of occupied territories of Sylvia communis. curruca.

Fig. 1 in Breeding Bird Assemblage In A Mosaic Of Urbanized Habitats In A Cenral European City

Fig. 1. Location of the study area (surrounded with a broken line) in the city of Wroclaw: A — borders of the study area; B — rivers; C — main roads; D — loosely built-up areas; E —densely built-up areas.

opencc-by-4.0Nov 2014View details →

Fig. 2 in Species Complexes Of Predatory Phytoseiid Mites (Parasitiformes, Phytoseiidae) In Green Urban Plantations Of Uman' (Ukraine)

Fig. 2. Phytoseiid mites occurrence on plants in green urban plantations of Uman': 1 — E. finlandicus, 2 — T. aceri, 3 — T. tiliarum, 4 — D. echinus, 5 — K. aberrans, 6 — P. incognitus, 7 — A. andersoni, 8 — P. soleiger, 9 — T. laurae, 10 — A. herbarius, 11 — G. longipilus, 12 — A. rademacheri.

opencc-by-4.0Mar 2014View details →

Fig. 1 in Species Composition And Distribution Of Oribatids (Acari, Oribatei) In Urbanized Biotopes Of Kyiv

Fig. 1. Cluster analisys of oribatid species composition in urbanized biotopes of Kyiv, Ukraine (groups 1—16 are given in Results and discussion).

opencc-zeroApr 2022View details →

Flying insect biomass is negatively associated with urban cover in surrounding landscapes

Aim In this study, we assessed the importance of local to landscape-scale effects of land cover and land use on flying insect biomass. Our main prediction was that insect biomass would be lower within more intensely used land, especially in urban areas and farmland. Location Denmark and parts of Germany. Methods We used rooftop-mounted car nets in a citizen science project ('InsectMobile') to allow for large-scale geographic sampling of flying insects. Citizen scientists sampled insects along 278 five km routes in urban, farmland and semi-natural (grassland, wetland and forest) landscapes in the summer of 2018. The bulk insect samples were dried overnight to obtain the sample dry weight/biomass. We extracted proportional land use variables in buffers between 50 and 1000 m along the routes and compiled them into land cover categories to examine the effect of each land cover, and specific land use types, on insect biomass. Results We found a negative association between urban cover and insect biomass at a landscape-scale (1000 m buffer) in both countries. In Denmark, we also found positive effects of all semi-natural land cover types, i.e. protected grassland (largest at the landscape-scale, 1000 m) and forests (largest at intermediate scales, 250 m). Protected grassland cover had a more positive effect on insect biomass than forest cover. The positive association between insect biomass and farmland was not clearly modified by any variable associated with farmland use intensity. The negative association between insect biomass and urban land cover appeared to be lessened by increased urban green space. Main conclusions Our results show that land cover has an impact on flying insect biomass with the magnitude of this effect varying across spatial scales. However, the vast expanse of grey space in urbanised areas has a direct negative impact on flying insect biomass across all spatial scales examined.

opencc-by-4.0Jul 2021View details →

Multi-taxa environmental DNA inventories reveal distinct taxonomic and functional diversity in urban tropical forest fragments

Urban expansion and associated habitat transformation drives shifts in biodiversity, with declines in taxonomic and functional diversity. Forests fragments within urban landscapes offer a number of ecosystem services, and help to maintain biodiversity and ecosystem functions. Here, we focus on a tropical forest environment, and on the soil biota. Using eDNA metabarcoding, we compare forest fragments within the city of Cayenne, French Guiana, with a neighbouring continuous undisturbed forest. We wished to determine if urban forest fragments conserve high levels of alpha and beta diversity as well as similar functional composition for plants, soil animals, fungi and bacteria. We found that alpha diversity is similar across habitats for plants and fungi, lower in urban forests for metazoans and higher for bacteria. We also found that urban forests communities differ from undisturbed forests in their taxonomic composition, with urban forests exhibiting greater turnover between fragments potentially caused by ecological drift and limited dispersal. However, their functional composition exhibited limited differences, with an enrichment of palms, arbuscular mycorrhizal fungi and bacteria and a depletion of climber plants and termites. Thus, although urban forest fragments do shelter soil biodiversity that differs from native forests, the losses of soil functions may be relatively limited. This study demonstrates the strong potential of a multi-taxa eDNA approach for rapid inventories across taxonomic kingdoms, in particular for cryptic soil diversity. It also demonstrates the key role of urban forest fragments in conserving biodiversity and ecosystem function, and points to a need for more systematic monitoring of these areas in urban management plans. For each of the 16 samples per plot, 15 g of soil was used for eDNA analyses. Extracellular DNA was extracted as described previously (Zinger et al., 2016; 2019), where each soil sample is added to 15ml of saturated phosphate buffer (Na2HPO4; 0.12m; pH ≈8) in 50ml Falcon tubes. This is placed in an agitator for 15 minutes, before a 2ml aliquot of the soil/phosphate buffer mixture is pipetted into an Eppendorf tube and centrifuged for five minutes at 13000 rcf. 500μL of the resulting supernatant is then recovered and used for the next extraction steps that are carried out with a commercial kit for soil DNA (NucleoSpin® Soil; Macherey-Nagel, Düren, Germany), skipping the lysis step and following manufacturer’s instructions. The DNA extract was recovered in 100 μL and diluted 10 times before being used as PCR template.  For each plot one DNA extraction negative control was performed adding up 17 extractions per plot. PCR amplifications were then conducted for four DNA molecular markers, with primers targeting either Viridiplantae (subsequently referred to as plants), Eukaryotes, Fungi or Bacteria (Table 1). For each marker, PCR amplification of samples occurred across 12 plates. Each PCR reaction was performed in a total volume of 20 μl and comprised 10 μl of AmpliTaq Gold Master Mix (Life Technologies, Carlsbad, CA, USA), 5.84 μl of Nuclease-Free Ambion Water (Thermo Fisher Scientific, Massachusetts, USA), 0.25 μM of each primer, 3.2 μg of BSA (Roche Diagnostic, Basel, Switzerland), and 2 μl of DNA template that was before 10-fold diluted to reduce the amounts of PCR inhibitors. Thermocycling conditions for each primer pair are indicated in Table 1.  A negative extraction control per site and a negative PCR control per PCR plate were amplified and sequenced in parallel with the regular samples. Positive controls were also included and consisted of mock communities of plants and fungi DNA (no mock communities were built for bacteria or eukaryotes here), which were used to guide choices in our data curation process. Two PCR replicates were performed for each sample and control. Amplification was conducted using a double indexing system strategy (Binladen et al. 2007) using a system of 32 by 36 octamers with at least five differences between them located at the 5’ end of each primer (Coissac 2012). In doing so, each PCR product had a unique combination of tags for both forward and reverse primers, allowing for the retrieval of sequence data for each sample. Ten wells per PCR plate were left empty to act as sequencing controls (non-used tag combinations) for downstream data curation (see below). PCR products were pooled and sequencing libraries were constructed using the Illumina TruSeq NanoPCRFree kit following the supplier’s instructions (Illumina Inc., San Diego, California, USA), except that the ligation product was not PCR amplified to limit tag-jump biases (Taberlet et al 2018). The libraries were then sequenced on different Illumina platforms (San Diego, CA, USA) depending on the marker considered (Table S1), using the paired-end technology. Bioinformatic analyses were performed on the GenoToul bioinformatics platform (Toulouse, France), with the OBITOOLS package (Boyer et al. 2016). First, ‘illuminapairedend’ was used to assemble paired-end reads. This algorithm is based on an exact alignment algorithm that considers the quality scores at all positions during the assembly process. Subsequently, we used the ‘ngsfilter’ command to identify and remove the primers and tags on each read, and assign reads to their respective samples. This program was used with its default parameters tolerating two mismatches for each of the two primers and no mismatch for the tags. Following this, sequencing reads were dereplicated using the ‘obiuniq’ command. Sequences of low quality (containing Ns or with paired-end alignment scores below 50) were excluded using the ‘obigrep’ command. The same command was used to exclude sequences represented by only one read (singletons) as they are more likely to be molecular artefacts (Taberlet et al. 2018). Sequences outside of the preset range were also discarded (Table 1). To remove PCR/sequencing errors as well as intraspecific variability, we built OTUs (Operational Taxonomic Units) using the ‘sumaclust’ clustering algorithm (Mercier et al. 2013), which considers the most abundant sequence of each cluster as the cluster representative.  OTUs were set at a sequence similarity threshold of 97% for eukaryotes, fungi and bacteria following the standards in microbial ecology, but this was lowered to 95% for plants since the eDNA target region is shorter (typically around 50 base pairs), where one mismatch inherently results in a lower percentage of similarity. To assign a taxon to plant and fungal OTUs, we built two reference sequence databases, one global, using the ecoPCR programme (Ficetola et al. 2010) and the plant / fungi specific markers on the European Molecular Biology Laboratory (EMBL; release 141), a second local, generated from specimens of fungi (Jaouen et al. 2019) and plants (see Zinger et al. 2019) collected in French Guiana. OTUs were then assigned a taxonomy, using OBITOOL’s ecotag programme (Boyer et al. 2016), which performs a global alignment of each OTU sequence (the query) against each reference. The reference taxon assigned to each OTU corresponds to the Last Common Ancestor of all the best-match sequences for the query. For taxonomic assignment of bacteria and eukaryote OTUs, the SILVA taxonomic database was used (version 1.3; Quast et al., 2012). Classification was performed by a local nucleotide BLAST search against the non-redundant version of the SILVA SSU Ref dataset (release 132; http://www.arb-silva.de) using blastn (version 2.2.30+; http://blast.ncbi.nlm.nih.gov/Blast.cgi) with standard settings (Camacho et al., 2009).  Eukaryote derived metazoan OTUs were then further assigned a taxonomy for Phyla identified at the Arthropoda, Annelida and Nematoda level using reference sequence databases built as above for these groups using the ecoPCR programme on EMBL release 141. Datasets were subsequently filtered to remove contaminants as well as artefacts such as PCR chimeras and remaining sequencing errors, following Zinger et al. (2019) and using routines now implemented in the metabaR R package (Zinger et al 2020b), in R version 3.6.1 (R Development Core Team, 2013). The filtering process consisted of four steps: (i) a negative control-based filtering. OTUs whose maximum abundance was found in extraction/PCR negative controls were removed from the dataset, as they were likely to be reagent/aerosol contaminants, better amplified in the absence of competing DNA fragments as it is the case in biological samples. (ii) a reference-based filtering. OTUs which are too dissimilar from sequences available in reference databases are potential chimeras generated during sequencing and amplification. In this study, we chose to set similarity thresholds at 95% for plants, 80% for bacteria and eukaryotes and due to the marker being more polymorphic, 65% for fungi. For plants and fungi, the remaining assignment was then verified with the local database, to confirm if assigned taxa also occurred in the local dataset, with preference given to local assignment. In addition, we removed all taxa that are not targeted by the primer used. (iii) an abundance-based filtering. This procedure targets incorrect assignment of a few numbers of sequences corresponding to true OTUs occurring to the wrong sample, a phenomenon called “tag-switching” (Esling et al. 2015), “tag jumps” (Schnell et al. 2015) or “cross-talk” (Edgar 2018). It consists in setting OTUs abundances to 0 in samples where their abundance represents < 0.03% of the total OTU abundance in the entire dataset. (iv) Finally, we conducted a PCR-based filtering by considering any PCR reaction that yielded less than 100 reads for plants, 1000 reads for fungi, bacteria and eukaryotes as non-functional, and removed them from the dataset. Data provided consists of 4 x OTU tables for each of the markers used to target different components of the soil biota, with rows representing each OTU, and columns the features of the OTU within the dataset, namely their id code, the number of read counts in the analysed dataset, their similarity score against the taxonomic dataset used to identify them, and when possible, a functional group assignment used in the manuscript. Details of these can be found above and in the manuscript and supplementary information. For each of the four datasets, we also provide a .rds file, corresponding to the processed dataset used in manuscript preparation. This is in the format of a metabaR list which includes PCR, Sample, Read count and the seperately provided OTU datasets. To facilitate interpretation, please refer to Zinger, L., Lionnet, C., Benoiston, A.S., Donald, J., Mercier, C. and Boyer, F., 2021. metabaR: an R package for the evaluation and improvement of DNA metabarcoding data quality. Methods in Ecology and Evolution, 12(4), pp.586-592. For the fungal (ITS) data, we also provide :  - the R1/R2 raw fastq files of the samples used in the paper + experimental controls - a tsv file containing the tag combinations corresponding to the samples/PCR replicates, to enable demultiplexing of data. - a csv file containing the description of each sample.

opencc-zeroDec 2021View details →

Characteristics of the urban sewer system and rat presence in Seattle

Rats are abundant and ubiquitous in urban environments. There has been increasing attention to the need for evidence-based, integrated rat management and surveillance approaches because rats can compromise public health and impose economic costs. Yet there are few studies that characterize rat distributions in sewers and there are no studies that incorporate the complexity of sewer networks that encompass multiple sewer lines, all comprised of their own unique characteristics. To address this knowledge gap, this study identifies sewer characteristics that are associated with rat presence in the city of Seattle's urban sewer system. We obtained sewer baiting data from 1752 geotagged manholes to monitor rat presence and constructed generalized additive models to account for spatial autocorrelation. Sewer rats were unevenly distributed across sampled manholes with clusters of higher rat presence at upper elevations, within sanitary pipes, narrower pipes, pipes at a shallower depth, and older pipes. These findings are important because identifying features of urban sewers that promote rat presence may allow municipalities to target areas for rat control activities and sewer maintenance. These findings suggest the need to evaluate additional characteristics of the surface environment and identify the factors driving rat movement within sewers, across the surface, and between the surface and the sewers.

opencc-by-4.0Jul 2022View details →

A Google Earth Engine code to analyze residential buildings' real estate values, summer surface thermal anomaly patterns and urban features: a Florence (Italy) case study

<ol> </ol> The layers included in the code were from the study conducted by the research group of CNR-IBE (Institute of BioEconomy of the National Research Council of Italy) and ISPRA (Italian National Institute for Environmental Protection and Research), published by the Sustainability journal (https://doi.org/10.3390/su14148412). Link to the Google Earth Engine (GEE) code (link: <a href="https://code.earthengine.google.com/715aa44e13b3640b5f6370165edd3002">https://code.earthengine.google.com/715aa44e13b3640b5f6370165edd3002</a>) You can analyze and visualize the following spatial layers by accessing the GEE link:  <ol> <li>Daytime summer land surface temperature (raster data, horizontal resolution 30 m, from Landsat-8 remote sensing data, years 2015-2019)</li> <li>Surface thermal hot-spot (raster data, horizontal resolution 30 m) was obtained by using a statistical-spatial method based on the Getis-Ord Gi* approach through the ArcGIS Pro tool.</li> <li>Surface albedo (raster data, horizontal resolution 10 m, Sentinel-2A remote sensing data, year 2017)</li> <li>Impervious area (raster data, horizontal resolution 10 m, ISPRA data, year 2017)</li> <li>Tree cover (raster data, horizontal resolution 10 m, ISPRA data, year 2018)</li> <li>Grassland area (raster data, horizontal resolution 10 m, ISPRA data, year 2017)</li> <li>Water bodies (raster data, horizontal resolution 2 m, Geoscopio Platform of Tuscany, year 2016)</li> <li>Sky View Factor (raster data, horizontal resolution 1 m, lidar data from the OpenData platform of Florence, year 2016)</li> <li>Buildings' units of Florence (shapefile from the OpenData platform of Florence) include data on the residential real estate value from the Real Estate Market Observatory (OMI) of the National Revenue Agency of Italy (source: https://www1.agenziaentrate.gov.it/servizi/Consultazione/ricerca.htm, accessed on 14 July 2022). Data on the characterization of the buffer area (50 m) surrounding the buildings are included in this shapefile [the names of table attributes are reported in the square brackets]: averaged values of the daytime summer land surface temperature [LST_media], thermal hot-spot pattern [Thermal_cl], mean values of sky view factor [SVF_medio], surface albedo [alb_medio], and average percentage areas of imperviousness [ImperArea%], tree cover [TreeArea%], grassland [GrassArea%] and water bodies [WaterArea%]. </li> </ol> Here attached the .txt file of the GEE code.    E-mail Giulia Guerri, CNR-IBE, giulia.guerri@ibe.cnr.it Marco Morabito, CNR-IBE, marco.morabito@cnr.it Alfonso Crisci, CNR-IBE, alfonso.crisci@ibe.cnr.it