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4,004 results for “In vivo”

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dryad32/100

Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

<p>Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.</p>

opencc-zeroNov 2022View details →
zenodo32/100

Underlying data for In vivo assessment of the relationship between oral lichen planus and antioxidant status of vitamins A, C, and E in diabetic and hypertensive patients: An observational study

<p>Underlying data for In vivo assessment of the relationship between oral lichen planus and antioxidant status of vitamins A, C, and E in diabetic and hypertensive patients: An observational study</p>

opencc-by-4.0Dec 2022View details →
zenodo32/100

STROBE checklist for: In vivo assessment of the relationship between oral lichen planus and antioxidant status of vitamins A, C, and E in diabetic and hypertensive patients: An observational study

<p>STROBE checklist for: In vivo assessment of the relationship between oral lichen planus and antioxidant status of vitamins A, C, and E in diabetic and hypertensive patients: An observational study</p>

opencc-by-4.0Dec 2022View details →
dryad32/100

Arginine-vasopressin expressing neurons in the murine suprachiasmatic nucleus exhibit a circadian rhythm in network coherence in vivo

<p>The suprachiasmatic nucleus (SCN) is composed of functionally distinct subpopulations of GABAergic neurons which form a neural network responsible for synchronizing most physiological and behavioral circadian rhythms in mammals. To date, little is known regarding which aspects of SCN rhythmicity are generated by individual SCN neurons, and which aspects result from neuronal interaction within a network. Here, we utilize in vivo miniaturized microscopy to measure fluorescent GCaMP-reported calcium dynamics in arginine vasopressin (AVP)-expressing neurons in the intact SCN of awake, behaving mice. We report that SCN AVP neurons exhibit periodic, slow calcium waves which we demonstrate, using in vivo electrical recordings, likely reflect burst firing. Further, we observe substantial heterogeneity of function in that AVP neurons exhibit unstable rhythms and relatively weak rhythmicity at the population level. Network analysis reveals that correlated cellular behavior, or coherence, among neuron pairs also exhibited stochastic rhythms with about 33% of pairs rhythmic at any time. Unlike single-cell variables, coherence exhibited a strong rhythm at the population level with time of maximal coherence among AVP neuronal pairs at CT/ZT 6 and 9, coinciding with the timing of maximal neuronal activity for the SCN as a whole. These results demonstrate robust circadian variation in the coordination between stochastically rhythmic neurons and interactions between AVP neurons in the SCN may be more influential than single-cell activity in the regulation of circadian rhythms. Furthermore, they demonstrate that cells in this circuit, like those in many other circuits<span></span>, exhibit profound heterogenicity of function over time and space.</p>

opencc-zeroJan 2023View details →
dryad32/100

Data from: The Reissner fiber under tension in vivo shows dynamic interaction with ciliated cells contacting the cerebrospinal fluid

<p>The Reissner fiber (RF) is an acellular thread positioned in the midline of the central canal that forms together with cerebrospinal fluid (CSF)-contacting neurons (CSF-cNs) an axial sensory system detecting curvature in the spinal cord. How these components interact is unknown. Using <em>in vivo</em> imaging in larval zebrafish, we show that RF is under tension and resonates dorsoventrally. Focal RF ablations trigger retraction and relaxation of the fiber cut ends, with larger retraction speeds for rostral ablations. We built a mechanical model that estimates RF stress diffusion coefficient at 10 mm<sup>2</sup>/s and reveals that tension builds up rostrally along the fiber. After RF ablation, CSF-cN spontaneous activity decreased and ciliary motility changed, suggesting physical interactions between RF and cilia projecting into the central canal. We observed that motile cilia were caudally-tilted and frequently interacted with RF. We propose that the numerous ependymal motile monocilia contribute to RF heterogenous tension via weak interactions. Our work demonstrates that under tension, the Reissner fiber dynamically interacts with motile cilia generating CSF flow and spinal sensory neurons.</p>

opencc-zeroMar 2023View details →
zenodo32/100

Assessing the Efficacy and Safety of White Birch Sap for Skin Whitening: In Vivo and In Vitro Investigations

<p><strong>Figure S1.</strong>&nbsp;Schematic diagram of fluorescence intensity of neutral-particle fluorescent zebrafish treated with WBS (50, 100 and 200&nbsp;&mu;g/mL).</p>

opencc-by-4.0May 2023View details →
zenodo32/100

Data associated with Cell Reports publication: Dura-Bernal et al. 2023, "Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics"

<p>This dataset includes experimental data used to constrain and validate the model, and model simulation output data. The source code for the associated M1 model and data analysis can be found here:&nbsp;https://github.com/suny-downstate-medical-center/M1_NetPyNE_CellReports_2023</p> <p>Please download the&nbsp;data_v2.zip file, which contains the most updated and complete version of the data.</p> <p>For more information please contact: salvador.dura-bernal@downstate.edu&nbsp;&nbsp;</p>

opencc-by-4.0May 2023View details →
zenodo32/100

QUEST-MM, QUEST(Subtract), QUEST(Met+Back) and QUEST(Met+MM) quantification results of in-vivo and simulated MRS human and rat brain spectra

<p>QUEST-MM, QUEST(Subtract), QUEST(Met+Back) and QUEST(Met+MM) quantification results of rat and human brain <em>in-vivo</em> and simulated spectra alongside with the metabolite profiles and the simulated 9.4T rat and 4T and 3T human brain spectra.</p> <p>All files can be loaded in jMRUI software version 5 and later.</p>

opencc-by-4.0Jun 2023View details →
zenodo32/100

Supplementary data for "Engineering PD-1-targeted small protein variants for in vitro diagnostics and in vivo PET imaging"

<p>Supplementary data for &quot;Engineering PD-1-targeted small protein variants for in vitro diagnostics and in vivo PET imaging&quot;. The docking.zip file contains ClusPro protein/protein docking results for all binding protein variants using either murine PD-1 structure (3bikB) or human PD-1 AF2 model as receptors. The pymol session contains data and scenes used to generate the&nbsp;Figures in the paper.</p>

opencc-by-4.0Jul 2023View details →
zenodo32/100

Fig. 7 in Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Fig. 7. Effect of 3β-(4-Methoxybenzoyloxy)-22β-senecioyloxy-olean-12-en-28-oic acid (10) on: (A) DMBA/TPA induced skin tumorigenesis, (B) growth of lesions.

opennotspecifiedFeb 2023View details →
zenodo32/100

Fig. 4 in Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Fig. 4. (a). 1Le9 receptor; 4(b). 3QA8 receptor: Indicating new cartoon and stick model along with binding cavity in yellow colour. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

opennotspecifiedFeb 2023View details →
zenodo32/100

Fig. 5. 3D in Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Fig. 5. 3D ribbon representation and zoom view: (A) Doxorubicin in the binding pocket of NF-κB (1Le9), (B) compound 10 in the binding pocket of NF-κB (1Le9), (C) Doxorubicin in the binding pocket of IKK-β (3QA8), (D) Compound 10 in the binding pocket of IKK-β (3QA8).

opennotspecifiedFeb 2023View details →
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Fig. 3 in Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Fig. 3. Cell viability of Lantadenes (1–2), reduced Lantadenes (3–4) and Lantadene ester derivatives (5–10) against: (A) A375 cell line, (B) A431 cancer cell line using SRB assay. Each point represents a mean value and SEM of 3 independent experiments. ANOVA was applied followed by Tukey's test.

opennotspecifiedFeb 2023View details →
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Fig. 7 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 7. Relative expression of six genes related to the biosynthesis of TAs, including farnesyl diphosphate synthase (FDS), squalene synthase (SQS), squalene epoxidases (SQE), lupeol synthase (LUS), β-amyrin synthase (BAS), and mixed function amyrin synthase (MFAS) genes in the different ploidy levels of in vitro and in vivo conditions of S. officinalis. Error bars are shown as standard deviation (n = 3).

opennotspecifiedSep 2021View details →
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Fig. 6 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 6. The observation of stomata characteristics in vivo plants of diploid (A1,2) and mixoploid (B1,2) of S. officinalis. Bars = 50 and 10 μm.

opennotspecifiedSep 2021View details →
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Fig. 5 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 5. The observation of stomata characteristics in vitro plants of diploid (A1,2), tetraploid (B1,2), and mixoploid (C1,2) of S. officinalis. Bars = 50 and 10 μm.

opennotspecifiedSep 2021View details →
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Fig. 2 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 2. Chromosome numbers of root tip cells from diploid plants 2n = 2x = 14 (A); and tetraploid plants 2n = 4x = 28 (B) of S. officinalis.

opennotspecifiedSep 2021View details →
zenodo32/100

Fig. 1 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 1. Histograms of flow cytometric analysis of diploid (A), tetraploid (B) and mixoploid (C) plants of S. officinalis.

opennotspecifiedSep 2021View details →
zenodo32/100

Fig. 4 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 4. The morphological characteristics of in vivo plants of diploid (A), and mixoploid (B) of S. officinalis.

opennotspecifiedSep 2021View details →
zenodo32/100

Fig. 8 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 8. Pearson correlation and the relationship between TAs contents of betulinic acid (BA), ursolic acid (UA), and oleanolic acid (OA) and gene expression related to triterpenic acids biosynthesis pathway including farnesyl diphosphate synthase (FDS), squalene synthase (SQS), squalene epoxidases (SQE), lupeol synthase (LUS), β-amyrin synthase (BAS), and mixed function amyrin synthase (MFAS) genes in different ploidy levels of in vitro and in vivo conditions of S. officinalis.

opennotspecifiedSep 2021View details →

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Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas
neuroscienceopenDocumentation, web resources, and API references are available online.
Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage
behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.
Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl
behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.
Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record