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1,574 results for “genome sequencing”
Complete Genome Sequence of an Aeromonas rivuli Strain Isolated from Ready-to-Eat Food - Data Files
<p>This dataset contains input and intermediate files of the bcgTree analysis described in the Schwartz <em>et al</em>. MRA manuscript entitled “Complete Genome Sequence of an <em>Aeromonas rivuli</em> Strain Isolated from Ready-to-Eat Food”.</p> <table> <tbody> <tr> <td> <p><strong>File name</strong></p> </td> <td> <p><strong>Description</strong></p> </td> </tr> <tr> <td> <p>‘<em>Aeromonadaceae</em> identifier’.fa</p> </td> <td> <p>Amino acid FASTA file of the translated CDS sequences of a strain X (bcgTree input file)</p> </td> </tr> <tr> <td> <p>full_alignment.concat.fa</p> </td> <td> <p>Alignment of the concatenated amino acid sequences of 107 single-copy core genes that is used for phylogenetic tree calculation in bcgTree (bcgTree intermediate file)</p> </td> </tr> </tbody> </table> <p> </p> <p>In the bcgTree files, the <em>Aeromonadaceae</em> sequences were named/abbreviated as follows:</p> <table> <tbody> <tr> <td> <p><strong>Sequence name in the bcgTree file</strong></p> </td> <td> <p><strong>Description</strong></p> </td> </tr> <tr> <td> <p>AAZUK01-1</p> </td> <td> <p><em>Tolumonas lignilytica </em>BRL6-1</p> </td> </tr> <tr> <td> <p>Aeromonas-caviae</p> </td> <td> <p><em>Aeromonas caviae </em>NCTC 12244</p> </td> </tr> <tr> <td> <p>Aeromonas-dhakensis</p> </td> <td> <p><em>Aeromonas dhakensis </em>CIP 107500</p> </td> </tr> <tr> <td> <p>Aeromonas-hydrophila</p> </td> <td> <p><em>Aeromonas hydrophila </em>ATCC 7966</p> </td> </tr> <tr> <td> <p>Aeromonas-rivuli</p> </td> <td> <p><em>Aeromonas rivuli </em>DSM 22539</p> </td> </tr> <tr> <td> <p>Aeromonas-rivuli-20-VB00005</p> </td> <td> <p><em>Aeromonas rivuli </em>20-VB00005</p> </td> </tr> <tr> <td> <p>Aeromonas-veronii</p> </td> <td> <p><em>Aeromonas veronii </em>CECT 4257</p> </td> </tr> <tr> <td> <p>CP001616-1</p> </td> <td> <p><em>Tolumonas auensis </em>DSM 9187</p> </td> </tr> <tr> <td> <p>JACHGR01-1</p> </td> <td> <p><em>Tolumonas osonensis </em>DSM 22975</p> </td> </tr> </tbody> </table>
Data from: Genome-wide sequence-based genotyping supports a nonhybrid origin of Castanea alabamensis
<p>The genus Castanea in North America contains multiple tree and shrub taxa of conservation concern. The two species within the group, American chestnut (Castanea dentata) and chinquapin (C. pumila sensu lato), display remarkable morphological diversity across their distributions in the eastern United States and southern Ontario. Previous investigators have hypothesized that hybridization between C. dentata and C. pumila has played an important role in generating morphological variation in wild populations. A putative hybrid taxon, Castanea alabamensis, was identified in northern Alabama in the early 20th century; however, the question of its hybridity has been unresolved. We tested the hypothesized hybrid origin of C. alabamensis using genome-wide sequence-based genotyping of C. alabamensis, all currently recognized North American Castanea taxa, and two Asian Castanea species at >100,000 single-nucleotide polymorphism (SNP) loci. With these data, we generated a high-resolution phylogeny, tested for admixture among taxa, and analyzed population genetic structure of the study taxa. Bayesian clustering and principal components analysis provided no evidence of admixture between C. dentata and C. pumila in C. alabamensis genomes. Phylogenetic analysis of genome-wide SNP data indicated that C. alabamensis forms a distinct group within C. pumila sensu lato. Our results are consistent with the model of a nonhybrid origin for C. alabamensis. Our finding of C. alabamensis as a genetically and morphologically distinct group within the North American chinquapin complex provides further impetus for the study and conservation of the North American Castanea species.</p>
Orca: Sequence-based modeling of genome 3D architecture from kilobase to chromosome-scale (Part1)
<p>This dataset (Part 1) provide the core resource files required for using the code of Orca, including models and the hg38 reference genome (resources_core.tar.gz), and the micro-C mcool files required for extracting the experimental observations (resources_mcools.tar.gz). Orca is a sequence-based deep learning modeling framework for multiscale genome 3D architecture.</p>
Single cell Iso-Sequencing enables rapid genome annotation for scRNAseq analysis
<p>Single <span>cell RNA sequencing (scRNAseq) is a powerful technique that continues to expand across various biological applications. However, incomplete 3' UTR annotations can impede single cell analysis resulting in genes that are partially or completely uncounted. Performing scRNAseq with incomplete 3' UTR annotations can hinder the identification of cell identities and gene expression patterns and lead to erroneous biological inferences. We demonstrate that performing single cell isoform sequencing (ScISOr-Seq) in tandem with scRNAseq can rapidly improve 3' UTR annotations. Using threespine stickleback fish (</span><em>Gasterosteus aculeatus</em><span>), we show that gene models resulting from a minimal embryonic ScISOr-Seq dataset retained 26.1% greater scRNAseq reads than gene models from Ensembl alone. Furthermore, pooling our ScISOr-Seq isoforms with a previously published adult bulk Iso-Seq dataset from stickleback, and merging the annotation with the Ensembl gene models, resulted in a marginal improvement (+0.8%) over the ScISOr-Seq only dataset. In addition, isoforms identified by ScISOr-Seq included thousands of new splicing variants. The improved gene models obtained using ScISOr-Seq lead to successful identification of cell types and increased the reads identified of many genes in our scRNAseq stickleback dataset. Our work illuminates ScISOr-Seq as a cost-effective and efficient mechanism to rapidly annotate genomes for scRNAseq.</span></p>
Figure 3. Bayesian maximum clade credibility tree constructed from whole mitochondrial genome sequences, with a in Evolutionary history of Sundaland shrews (Eulipotyphla: Soricidae: Crocidura) with a focus on Borneo
Figure 3. Bayesian maximum clade credibility tree constructed from whole mitochondrial genome sequences, with a focus on Bornean Crocidura. Reconstructed with BEAST2. Dates are indicated on nodes and bar on bottom. Nodes used to calibrate the dating are marked with a black square. Uropsilus and Soricinae outgroups were included in the phylogenetic analysis for calibration purposes but removed from the figure for clarity. Since most nodes are highly supported (PP> 0.95), only less supported nodes (0.95 0.80) are marked with an orange circle. Colours on the vertical bar indicate geographical origin of sampled animals, and colors match the map. Samples from north of Kra are shown in grey.
Low-coverage whole-genome sequencing reveals molecular markers for spawning season and sex identification in Gulf of Maine Atlantic cod (Gadus morhua, Linnaeus 1758)
<p class="CxSpFirst">Atlantic cod (<i>Gadus morhua</i>,<i> </i>Linnaeus 1758) in the western Gulf of Maine are managed as a single stock despite several lines of evidence supporting two spawning groups (spring and winter) that overlap spatially, while exhibiting seasonal spawning isolation. Low-coverage whole genome sequencing was used to evaluate the genomic population structure of Atlantic cod spawning groups in the western Gulf of Maine and Georges Bank using 222 individuals collected over multiple years. Results indicated low total genomic differentiation, while also showing strong differentiation between spring and winter spawning groups at specific regions of the genome. Guided regularized random forest and ranked <i>F</i><sub>ST</sub> methods were used to select panels of single nucleotide polymorphisms (SNPs) that could reliably distinguish spring and winter-spawning Atlantic cod (88.5% assignment rate), as well as males and females (95.0% assignment rate) collected in the western Gulf of Maine. These SNP panels represent a valuable tool for fisheries research and management of Atlantic cod in the western Gulf of Maine that will aid investigations of stock production and support accuracy of future assessments.</p>
Draft Genome Sequence of Pseudomonas sp. Strain MWU13-2922, Isolated from a Wild Cranberry Bog in Truro, Massachusetts
<p>Annotated genome of Pseudomonas sp. MWU13-2922</p>
Draft Genome Sequences of Aquitalea sp strain MWU-14.2238
<p>Annotated genome of Aquitalea sp MWU-14.2238. </p>
Draft Genome Sequences of Pseudomonas sp. Strain MWU12-2233, Isolated from a Wild Cranberry Bog in Provincetown, Massachusetts
<p>Annotated genome of Pseudomonas sp. MWU12-2233.</p>
Draft Genome Sequences of Pseudomonas sp strain MWU-15.20650
<p>Annotated genome of Pseudomonas sp. MWU 15-20650.</p>
Draft Genome Sequences of Aquitalea sp strain MWU-14.2238
<p>Annotated genome of Aquitalea sp MWU-14.2238. </p>
Draft Genome Sequence of Pseudomonas sp. strain MWU13.2105, isolated from Wild Cranberry Bog in Truro, Massachusetts
<p>Annotated genome of Pseudomonas sp. strain MWU13.2105.</p>
Draft Genome Sequence of Pseudomonas sp. strain MWU13.2100, isolated from Wild Cranberry Bog in Truro, Massachusetts
<p>Annotated genome of Pseudomonas sp. strain MWU13.2100.</p>
Substitution mutational signatures in whole-genome-sequenced cancers in the UK population, Mutational Signatures Data
<p>This uploads contains the mutational signature data from the article <strong>Substitution mutational signatures in whole-genome-sequenced cancers in the UK population</strong>,<strong> </strong><em>Science</em>, doi:10.1126/science.abl9283, 2022.</p>
Genome wide mRNA sequencing data in macrophages without and with CX-5461 treatment
<p class="MsoNormal"><span>CX-5461, a novel selective RNA polymerase I inhibitor, shows potential anti-inflammatory and immunosuppressive activities. However, the molecular mechanisms underlying the inhibitory effects of CX-5461 on macrophage-mediated inflammation remain to be clarified. In the present study, we attempted to identify the systemic biological processes which were modulated by CX-5461 in inflammatory macrophages. Primary peritoneal macrophages were isolated from normal Sprague Dawley rats, and primed with lipopolysaccharide or interferon-gamma. Genome-wide RNA sequencing was performed. The study suggests that limiting cell proliferation predominates in the inhibitory effects of CX-5461 on macrophage-mediated inflammation. </span></p>
Data from: Whole genome sequencing and phylogenomic analysis show support for the splitting of genus Pythium
<p>The genus <em>Pythium</em><span> (nom. cons.) sensu lato (s.l.) is composed of many important species of plant pathogens. Early molecular phylogenetic studies suggested paraphyly of </span><em>Pythium</em><span>, which led to a formal proposal by Uzuhashi and colleagues in 2010 to split the genus into </span><em>Pythium</em><span> sensu stricto (s.s.), </span><em>Elongisporangium, Globisporangium, Ovatisporangium</em><span> (= </span><em>Phytopythium</em><span>), and </span><em>Pilasporangium</em><span> using morphological characters and phylogenies of the mt cytochrome </span><em>c</em><span> oxidase subunit 2 (</span><em>cox2</em><span>) and D1–D2 domains of nuc 28S rDNA. Although the split was fairly justified by the delineating morphological characters, there were weaknesses in the molecular analyses, which created reluctance in the scientific community to adopt these new genera for the description of new species. In this study, this issue was addressed using phylogenomics. Whole genomes of 109 strains of </span><em>Pythium</em><span> and close relatives were sequenced, assembled, and annotated. These data were combined with 10 genomes sequenced in previous studies. Phylogenomic analyses were performed with 148 single-copy genes represented in at least 90% of the taxa in the data set. The results showed support for the division of </span><em>Pythium</em><span> s.l. The status of alternative generic names that have been used for species of </span><em>Pythium</em><span> in the past (e.g., </span><em>Artotrogus, Cystosiphon, Eupythium, Nematosporangium, Rheosporangium, Sphaerosporangium</em><span>) was investigated. Based on our molecular analyses and review of the </span><em>Pythium</em><span> generic concepts, we urge the scientific community to adopt the generic names </span><em>Pythium, Elongisporangium, Globisporangium</em><span>, and their concepts as proposed by Uzuhashi and colleagues in 2010 in their work going forward. In order to consolidate the taxonomy of these genera, some of the recently described </span><em>Pythium</em><span> spp. are transferred to </span><em>Elongisporangium</em><span> and </span><em>Globisporangium</em><span>.</span></p>
Xcc BrA1 re-sequenced genome contigs, annotation file and Trinitiy De-nove assembled DEG's
<p>Assembled genome contigs of Xcc strain BrA1 (Behlau et al 2017) which was re-sequenced by our lab and used in a copper stress Transcriptome study. A hybrid RNA-seq analysis pipeline was used and the DEG Trinity De-novo assembled transcripts are also included.</p>
Genome sequences of Rhizopogon roseolus, Mariannaea elegans, Myrothecium verrucaria, and Sphaerostilbella broomeana and the identification of biosynthetic gene clusters for fungal peptide natural products
<p>Data to accompany the paper, detailed in manifest.txt</p>
Neutral and adaptive drivers of genomic change in introduced brook trout (Salvelinus fontinalis) populations revealed by pooled sequencing
<p>Understanding the drivers of successful species invasions is important for conserving native biodiversity and for mitigating the economic impacts of introduced species. However, whole-genome resolution investigations of the underlying contributions of neutral and adaptive genetic variation in successful introductions are rare. Increased propagule pressure should result in greater neutral genetic variation, while environmental differences should elicit selective pressures on introduced populations, leading to adaptive differentiation. We investigated neutral and adaptive variation among nine introduced brook trout (<em>Salvelinus fontinalis</em>) populations using whole-genome pooled sequencing. The populations inhabit isolated alpine lakes in western Canada and descend from a common source, with an average of ~19 (range of 7-41) generations since introduction. We found some evidence of bottlenecks without recovery, no strong evidence of purifying selection, and little support that varying propagule pressure or differences in local environments shaped observed neutral genetic variation differences. Putative adaptive loci analysis revealed non-convergent patterns of adaptive differentiation among lakes with minimal putatively adaptive loci (0.001%-0.15%) that did not correspond with tested environmental variables. Our results suggest that (i) introduction success is not always strongly influenced by genetic load, (ii) observed differentiation among introduced populations can be idiosyncratic, population-specific, or stochastic, and (iii) conservatively, in some introduced species, colonization barriers may be overcome by support through one aspect of propagule pressure or benign environmental conditions.</p>
Sequences of Staudtia kamerunensis obtained through low coverage whole genome skimming
<p>The impact of Pleistocene climatic oscillations on the biodiversity of African tropical rain forests remains poorly understood, and the Congo Basin is particularly understudied. We aim to elucidate how Pleistocene climatic oscillations shaped lowland tropical rain forests by investigating the intraspecific diversity and evolutionary history of a widespread tree species, <em>Staudtia kamerunensis</em> Warb.</p> <p>We sequenced 88 individuals of <em>Staudtia kamerunensis</em> and 1 of <em>Staudtia pterocarpa</em> using a genome skimming approach. We used maximum likelihood and Bayesian inference to infer the plastid phylogeny. We estimated the time of speciation and differentiation, genetic diversity, and we employed a continuous phylogeographic approach to infer the dispersal history of its plastid lineages.</p> <p>We identified five plastid lineages that diverged during the Early or Middle Pleistocene and are parapatric, suggesting past population fragmentation. Four lineages are endemic to Lower Guinea, and one spans the Congo Basin. We found contrasting patterns of expansion in the two regions, with a rapid and recent range expansion of the Congolian lineage in the last 200,000 years, while the spread of the Lower Guinean lineages was substantially slower.</p> <p>The contrasting demographic histories between eastern and western lineages, associated with contrasted levels of plant species richness and rates of endemism, suggest that forest cover was more stable in Lower Guinea during the Late Pleistocene than in Congolia, where the biodiversity might have been eroded before the forest re-expanded in the Congo basin. This study illustrates how a continuous phylogeographic inference approach, mostly applied so far for inferring the spread of fast-evolving pathogens over months or years, can provide new insights to reconstruct the dispersal history of tropical tree species over thousands or millions of years.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.