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Figure 1 in A new damsel-dragonfly from the Mesozoic of China with a hook-like male anal angle (Odonata: Isophlebioptera: Campterophlebiidae)

Figure 1. Angustiphlebia mirabilis gen. nov. et sp. nov. (A) Photograph of holotype specimen CNU-ODO-NN2011016, part only; (B) photograph of forewing; (C) line drawing of forewing, part and counterpart combined; (D) photograph of hind wing; (E) drawing of hind wing. Scale bars represent 10 mm.

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FIGURE 2. Hexabothriid sucker sclerite hook morphological measurements. A in Towards addressing the current state of confusion within the Hexabothriidae Price, 1942 (1908): Callorhynchocotyle Suriano & Incorvaia, 1982 (Monogenea: Hexabothriidae) re-visited, with the preliminary evaluation of novel parameters for measuring haptoral armature of hexabothriids

FIGURE 2. Hexabothriid sucker sclerite hook morphological measurements. A. Hook length; line a–b, hook aperture; line b– c, hook base width; line a–c. B. Hook curve length; line e–f. C. Hook aperture angle (x) from vertex (f) by rays f–b, f–c.

FIGURE 1. A in The rediscovery of Impatiens sigmoidea Hook. f. (Balsaminaceae) in Guizhou, China after more than 100 years

FIGURE 1. A Lectotype of Impatiens sigmoidea (E. M. Bodinier & J. Laborde 2688, E00313668) B Isolectotype (P00780758).

FIGURE 3. Impatiens sigmoidea. A–B Habit C in The rediscovery of Impatiens sigmoidea Hook. f. (Balsaminaceae) in Guizhou, China after more than 100 years

FIGURE 3. Impatiens sigmoidea. A–B Habit C Flower in face view D Flower in lateral view E Flowering branch F Base of stem and roots G Leaves H Inflorescences I–J Flower K Dissected flower L Bracts M Dorsal petals. Photographs by XIN-XIANG BAI.

FIGURE 5. Morphological comparison between Impatiens sigmoidea and I. lasiophyton. A–C I. sigmoidea. A–B Habit C Dissected flower. D–F I. lasiophyton. D in The rediscovery of Impatiens sigmoidea Hook. f. (Balsaminaceae) in Guizhou, China after more than 100 years

FIGURE 5. Morphological comparison between Impatiens sigmoidea and I. lasiophyton. A–C I. sigmoidea. A–B Habit C Dissected flower. D–F I. lasiophyton. D Flower in face view E Flower in lateral F Dissected flower. Photographs by XIN-XIANG BAI.

FIGURE 2. A in The rediscovery of Impatiens sigmoidea Hook. f. (Balsaminaceae) in Guizhou, China after more than 100 years

FIGURE 2. A Syntype of Impatiens sigmoidea (J. P. Cavalerie 287, E00313667) B Isotype of I. labordei (E. M. Bodinier & J. Laborde 2689, E00313666) C Holotype of I. labordei (E. M. Bodinier & J. Laborde 2689, P00780709).

FIGURE 4. Impatiens sigmoidea. A Flowering branch B in The rediscovery of Impatiens sigmoidea Hook. f. (Balsaminaceae) in Guizhou, China after more than 100 years

FIGURE 4. Impatiens sigmoidea. A Flowering branch B Base of stem and roots C Flower in face view D Flower in lateral view E Dissected flower F Anthers with pedicel and bract. Drawn by YI CHEN.

Fig. 6 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 6. Heatmap representing organ specific significantly enriched genes corresponding to (A) Isosteroidal alkaloid biosynthesis, (B) Sucrose and starch metabolism, (C) UGTs and CYPs, (D) aquaporins, (E) ABC transporters, (F) Transcription factor and Transposable elements. The red-blue scale represents positive enrichment (red) and negative enrichment (Blue) of transcripts. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 5. Significantly enriched transcripts (nodes) in predicted interactome network. (A) Steroidal alkaloid biosynthesis pathways (B) Isopentenyl diphosphate biosynthesis pathway, (C) Sucrose and starch metabolic pathways and (D) Aquaporins. The nodes encircled in red color represents higher enrichment in the bulb while the nodes encircled in grey and blue represents higher enrichment in arial organs (leaf and stem). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 7 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 7. qRT-PCR expression-based validation of RNA-seq data using potential 20 genes involved in isosteroidal alkaloid biosynthetic pathway. (A) Bulb vs. Leaf, (B) Leaf vs. Stem and (C) Bulb vs. Stem.

Fig. 3 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 3. Significant KEGG pathway enrichment analysis in tissues from aerial organs (leaf & Stem) and bulb. The green colour enrichment indicates higher expression in aerial tissue while pink represents higher enrichment in bulb. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 4. Transcriptional protein-protein interactome (PPIN) prediction in F. roylei along the network statistics. (A) Overall prediction of PPI network based on significantly differential expressed transcripts. Spatial PPI network prediction of significantly enriched transcripts in (B) Bulb, (C) Leaf and (D) Stem.

Fig. 1 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 1. Summary of organ specific spatial transcriptome sequencing in F. roylei. (A): Overall quality filtering of sequenced reads; (B): Tissue specific high quality filtered reads obtained after removing low quality/adaptor contaminated sequences; (C): Assembly statistics details; (D): Venn diagram representing functional annotation with six different public protein databases.

Fig. 8 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 8. Representation of isosteroidal alkaloid biosynthesis pathway in F. roylei and heat map representing expression of genes in Stem, Leaf and Bulb tissue using red-blue scale (red: positive enrichment and Blue: negative enrichment of transcripts). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 2. Clustering of 2488 significant differentially expressed transcripts in leaf, stem and bulb in F. roylei (A) sub-cluster 1 represent transcripts with significant higher expression in bulb; (B): Sub-cluster 2 in Stem and (C): Sub-cluster 3 in leaf; (D): Pearson's correlation of organ specific significant differentially expressed clustered transcripts in leaf, stem and bulb tissues.

Fig. 2 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb

Fig. 2. (A–E) Differential gene expression analysis in comparative F. roylei transcriptome: (A) Heat-map showing differential gene expression in the bulb (PKW) vs callus (PK2); bulb (PKW) vs in vitro regenerated plantlets (PK1) and callus (PK2) vs in vitro regenerated plantlets (PK1); (B) Venn diagram represents the differential gene expression in PKW vs PK1; PKW vs PK2; PK2 vs PK1, (C–E) Volcano plots represents the differential gene expression in PKW vs PK1; PKW vs PK2; PK2 vs PK1 as colour description image, where p-value & log2 fold-change in red colour represents genes with log2 fold-change cut off 2 and p-value <=0.05; p-value in blue colour represents genes with no cut off on log2 fold-change and p-value <=0.05. Whereas, log2 fold-change in green colour represents genes with fold-change cut off 2 but no p-value cut off and non-significant (NS) in grey colour represents genes with no filter on log2 fold-change and p-value, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb

Fig. 1. (A–F) Functional annotations and unigenes classification of comparative F. roylei transcriptome: (A) Unigenes annotation with top 15 different plant species; (B) Top 5 pathway representation as per Kyoto Encyclopedia of Genes and Genomes; (C) Gene Ontology classification under the cellular component, molecular function, and biological process categories; (D) COG (Cluster of Orthologous Groups of proteins) classification into nine different categories; (E) Unigenes classification into major transcription factor families; (F) Gene family and sub-family classification using TAIR database.

Fig. 4 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb

Fig. 4. Comparative expression pattern validation for the sipeimine biosynthetic pathway genes as obtained from RNA-Seq data and qRT-PCR.

Fig. 3 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb

Fig. 3. Proposed sipeimine biosynthetic pathways in F. roylei. Heat-map showing gene expression in the bulb, callus, and regenerated plantlets. The genes were mapped using TPM (transcripts per kilobase million) values and colour-coded by increasing relative expression. The broken dotted arrow represents putative terminal biosynthesis steps. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)