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Figures 31-33 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 31-33 Quasicalathus, light microscopic images of the holotypes of Q. agonicollis sp. nov. (31.) and Q. conservans sp. nov. (32, 33.). 31. Ventral side of head showing chaetotaxy of mentum; 32. General view of the amber piece with fossil in dorsal view; 32. Left lateral view. Abbreviations: ce – compound eye; el – elytron; ems – external seta of submentum; gu – gula; ims – internal seta of submentum; msf – mesofemur; mt – mentum; mtf – metafemur; prf – profemur; pt – pronotum.
Figures 47-51 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 47-51 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of specimen "Groehn 7889". 47. Dorsal aspect; 48. Left lateral aspect; 49. Ventral aspect; 50. Pronotum (the arrows point to the insertions of the lateral setae); 51. Prosternum (for better view the prolegs are partly removed using the clipping plane function of Amira software. Abbreviations: cx – procoxa; psp – prosternal process; tr – protrochanter.
Figures 6-12 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 6-12 Quasicalathus elpis (Ortuño and Arillo 2009), light microscopic images of specimens "Groehn 7889" (6–8.) and "Groehn 7962" (9–12.). 6, 10. General view of the amber pieces (in Fig. 6, only the part of the large amber piece bearing the Quasicalathus fossil is shown); 7. Ventral side of body; 8. Left mesotarsi iv + v; 9. Pronotum and anterior part of elytra, left side of body; 11, 12. Medial part of left elytron (Fig. 12 shows the enlarged part of the elytron marked by the white frame in Fig. 11; the white arrow points to the insertion of the discal seta). Abbreviations: bs – insertion of the pronotal laterobasal seta; hm – humerus; I–VIII – elytral intervals 1–8.
Figures 34-38 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 34-38 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of specimens "Groehn 4879" (34–37.) and "Groehn 7814" (38.). 34. Dorsal aspect; 35. Right lateral aspect; 36. Ventral aspect; 37, 38. Prosternum and basal portions of prolegs. Abbreviations: cx – procoxa; fm – profemur; psp – prosternal process; tr – protrochanter.
Figures 81-89 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 81-89 Quasicalathus conservans sp. nov., volume rendering of the holotype. 81. Head, dorsal aspect (the arrows point to the insertions of the supraorbital setae); 82. Head, ventral aspect; 83. Left external part of metathorax, ventral view; 84. Submentum (the arrows point to the insertions of the four lateral setae); 85. Posterior part of prosternum and procoxae; 86. Posterior part of metasternum and metacoxae; 87–89. Preserved remains of the aedeagus (87. Right lateral aspect; 88. Dorsal aspect; 89. Left lateral aspect). Abbreviations: bb – basal bulb of aedeagal median lobe; ce – compound eye; cxp – metacoxal plate; eph – partly evaginated lobes of endophallus; gu – gula; mem – metepimeron; mes – metepisternum; mtt – mentum tooth; mv – metaventrite; pcx – procoxa; pmr –preserved distal part of right paramere of aedeagal median lobe; psp – prosternal process; sc – scutellum; sps – setae of sensory pit; tl – terminal lamella of aedeagal median lobe.
Figures 61-64 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 61-64 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of specimens "MAIG 76" (61–63.) and "GZG 16185" (64.); 61. Dorsal aspect; 62. Right lateral aspect; 63, 64. Pronotum (the pronotal outline on left side is highlighted by dotted line in Fig. 64).
Figures 39-46 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 39-46 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of specimen "Groehn 7814" using different grey scales of the Amira software. 39. Dorsal aspect; 40. Lateral aspect. The displaced aedeagus (highlighted by red colour) was separated by the segmentation function of Amira software in Figures 39 and 40; 41. Head (the arrows point to the insertions of the supraorbital setae); 42. Pronotum (the arrows point to the insertions of the lateral setae); 43–46. Remains of the aedeagus in right lateral aspect (43.); Left lateral aspect (44.); Left lateral aspect (45.); Dorsal aspect (46.). The distal margins of the styloid apophysis of the right paramere in Fig. 43 and the lobate apophysis of the left paramere in Fig. 45 are highlighted by red dotted lines. Abbreviations: bb – basal bulb of aedeagal median lobe; os – distal ostium; pml – left paramere; pmr – right paramere; tl – terminal lamella of median lobe.
Figures 78-80 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 78-80 Quasicalathus conservans sp. nov., volume rendering of the holotype using different grey scales of the Amira software. 78. Dorsal aspect; 79. Left lateral aspect (aed – aedeagus); 80. Ventral aspect; the aedeagus (highlighted by red colour) was separated by the segmentation function of Amira software.
Figures 52-57 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 52-57 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of specimen "Groehn 7889". 52. Head, ventral aspect (the arrows point to the insertions of the setae near base of mentum tooth and on submentum); 53. Abdomen, left lateral aspect (the arrows point to the insertions of the setae on ventrites IV, V, and VI); 54. Metacoxal area (the arrows point to the insertions of the coxal setae); 55. Apical gonocoxites, ventral aspect; 56. Apical gonocoxites, dorsal aspect; 57. Gonocoxites and remains of the bursa copulatrix (the latter was highlighted by red colour using the segmentation function of Amira software). Abbreviations: at – apical tooth of retinacle; kes – metathoracic katepisternum; cx – metacoxa; cxp – metacoxal plate; des – dorsal ensiform setae; ep – elytral epipleuron; fm – metafemur; gu – gula; mdl – left mandible; gx1 – basal gonocoxite; gx2 – apical gonocoxite; mdr – right mandible; mo – molar; mt – mentum; sp – sensory pit; tr – metatrochanter; ves – ventral ensiform setae; v3, v4, v5, v6 – ventritres III, IV, V, VI.
Figures 73-77 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 73-77 Quasicalathus agonicollis sp. nov., volume rendering of specimen "GZG 16188". 73. Dorsal aspect; 74. Basal portion of pronotum and anterior part of elytra (right side of body; the arrows point to the insertions of the pronotal basolateral seta and the parascutellar seta); 75. Prosternum with basal portion of prolegs; 76. Left apical gonocoxite, ventral aspect; 77. Gonocoxites, ventral aspect. Abbreviations: cx – procoxa; des – dorsal ensiform setae; fm – profemur; gx1 – basal gonocoxite; gx2 – apical gonocoxite; hm – humerus; pst – prosternum; sc – scutellum; sps – setae of sensory pit; tr – protrochanter; ves – ventral ensiform setae.
Figures 13-17 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 13-17 Quasicalathus elpis (Ortuño and Arillo 2009), light microscopic images of specimens "CCHH 952" (13–15.) and "OSAC 269" (16, 17.). 13. Dorsal view of body; 14. Pronotum and anterior part of elytra showing the markedly concave basal margin and projected humeri (the white arrows point the insertion pores of the parascutellary setae); 15, 17. General view of the amber pieces; 16. Posterior part of left elytron (the white arrow points to the insertion of the discal seta).
Figures 18-24 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 18-24 Quasicalathus, light microscopic images of Q. elpis Ortuño & Arillo, 2009 (18–20.) and Q. agonicollis sp. nov. (21–24.). 18. General view of the amber piece "MAIG 76" (only that part of the large amber piece bearing the Quasicalathus fossil is shown; the fossil is widely covered by milky coating); 19. General view of the two fragments of specimen "GZG 16185"; the left one bears only the negative imprint of the left elytra on the inclusion wall; 20. Right anterior part of body of specimen "GZG 16185" showing part of head, pronotum and humerus; 21, 22. General view of the amber piece "GZG 16188" (21. With fossil in dorsal view; 22. In ventral view); 23. Anterior part of specimen "GZG 16188"; 24. Head of specimen "GZG 16188". Abbreviations: a1–a6 – antennomeres 1–6; as – anterior supraorbital seta; bs – pronotal laterobasal seta; ms – pronotal lateral seta; ps – posterior supraorbital seta.
Figures 58-60 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 58-60 Quasicalathus elpis (Ortuño and Arillo 2009), volume rendering of the dorsal aspects of specimens "Groehn 7962" (58.), "CCHH 952" (59.), and "OSAC 265" (60.).
Figures 65-72 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 65-72 Quasicalathus agonicollis sp. nov., volume rendering of the holotype using different grey scales of the Amira software. 65. Dorsal aspect (the negative imprint of the fossil on the inclusion wall is shown); 66. Basal portion of pronotum and anterior part of elytra (positive of the fossilized beetle is shown; the arrows point to the insertions of the pronotal basolateral setae and the parascutellar setae); 67. Metacoxa (the arrows point to the insertions of the three coxal setae each side); 68–71. Aedeagus in dorsal aspect (68.), Right lateral aspect (69.), Ventral aspect (70.), Left lateral aspect (71.); The remains of the parameres are coloured (red: left paramere; green: right paramere); 72. left lateral aspect of beetle body; the aedeagus (highlighted by red colour) was separated by the segmentation function of Amira software. Abbreviations: bb – basal bulb of aedeagal median lobe; hm – humerus; os – distal ostium of median lobe; sc – scutellum; tl – terminal lamella of median lobe.
Figures 1-5 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 1-5 Quasicalathus elpis (Ortuño and Arillo 2009), light microscopic images of specimens "Groehn 4879" (1–3.) and "Groehn 7814" (4, 5.). 1, 5. General view of the amber pieces; 2. Ventral side of head (the white arrow points to the mentum tooth; note that the mentum is somewhat detached from the head capsule); 3. Pronotum and anterior part of elytra showing the markedly concave basal margin and projected humeri; 4. Right lateral view of body.
Figures 25-30 from: Schmidt J, Scholz S, Will K (2022) Character analysis and descriptions of Eocene sphodrine fossils (Coleoptera, Carabidae) using light microscopy, micro-CT scanning, and 3D imaging. Deutsche Entomologische Zeitschrift 69(1): 19-44. https://doi.org/10.3897/dez.69.79931
Figures 25-30 Quasicalathus agonicollis sp. nov., light microscopic images of specimen "GZG 16188" (25–26.) and the holotype (28–30.). 25. Posterior part of pronotum and anterior part of elytra, right side; 26. Anterior part of fifth interval of left elytra showing microsculpture; 27. Posterior part of elytra (the arrows point to the insertions of the discal setae); 28. General view of the amber piece; 29. Right dorsal view of beetle body (the arrow points to the insertion of the discal seta on left elytron); 30. Left ventral view. Abbreviations: bs – insertion of the pronotal laterobasal seta; hm – humerus; m – mite (syninclusion).
Confocal and micro-CT data of Crenossidium slipinskii, holotype, NIGP180053
<p>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices and X-ray microtomography (micro-CT) slices for the holotype of <em>Crenossidium slipinskii</em> (NIGP180053) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "The first fossil of Nossidiinae From mid-Cretaceous amber of northern Myanmar (Coleoptera: Ptiliidae)" (DOI:10.3389/fevo.2022.911512).</p> <p>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using the 488 nm (Argon) laser excitation line. The original CZI files are provided, which could be opened by the ZEISS ZEN software.</p> <p>Micro-CT data were obtained with a Zeiss Xradia 520 Versa 3D X-ray microscope. Scanning parameters were as follows: isotropic voxel size, 1.7807 μm; power, 3 W; acceleration voltage, 40 kV; exposure time, 6 s; projections, 3001. The TIFF stack is provided.</p>
Text-fig. 5. Anacardiaceae (a–m), Burseraceae (n–q). Scale bars = 1 cm. a–f: Pentoperculum sp. a–c: USNM PAL 772360. a: Lateral view of endocarp, apex up; three germination valves visible, the central clearly displaying the bipartite nature of the valve, reflected light, palladium coated. b: Apical view displaying six locules, with two preserved germination valves at the lower left, reflected light, palladium coated. c: Basal view of the endocarp, the locules suggested by swellings; note point of attachment, micro-CT scan surface rendering. d–f: Pentoperculum sp. USNM PAL 772359, reflected light, palladium coated. d: Lateral view of a probable 6-loculed endocarp, apex up; a single intact germination valve in the center, displaying the central lineation that divides it in two. e: Apical view; two bi-partite germination valves are visible, indicated by arrows to the middle cleavage line of two of the valves. f: Basal view, the locules suggested by the undulations in the margin. g–i: Indet. Spondioideae. USNM PAL 772358, reflected light, palladium coated. g: Lateral view of multi-locular endocarp, apex up. h: Apical view showing finely punctuate surface and peripheral locule cavities. i: Basal view. j–m: Cf. Pleiogynium USNM PAL 772357. j: Lateral view of the multi-locular endocarp, apex up; note intact germination valve on left and exposed locule facing the viewer, micro-CT scan surface rendering. k: Lateral view, rotated about 30° from (j), showing three exposed locules, reflected light, palladium coated; note bipartite locule lining at center. l: Apical view of the multilocular endocarp; the locule with intact germination valve at the upper right, reflected light, palladium coated. Arrows to each locule. m: Basal view showing central point of attachment and prominent radiating ridges aligned with the locules, micro-CT scan surface rendering. n–q: Canarium, USNM PAL 772361. Scale bar = 1 cm. n: Lateral view of endocarp directly facing one germination valve flanked by two strong ridges; apex up; specimen coated in sodium nitrate and photographed by R. A. Scott. o: Lateral view facing one of the three pronounced ridges, flanked to the left and right by two germination valves; apex up. p: Apical view displaying the three strong ridges, arching over the apex and flanking three deep embayments, covered with germination valves. q: Basal view, the three ridges being less pronounced. o–q: Reflected light, palladium coated. in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.
Text-fig. 5. Anacardiaceae (a–m), Burseraceae (n–q). Scale bars = 1 cm. a–f: Pentoperculum sp. a–c: USNM PAL 772360. a: Lateral view of endocarp, apex up; three germination valves visible, the central clearly displaying the bipartite nature of the valve, reflected light, palladium coated. b: Apical view displaying six locules, with two preserved germination valves at the lower left, reflected light, palladium coated. c: Basal view of the endocarp, the locules suggested by swellings; note point of attachment, micro-CT scan surface rendering. d–f: Pentoperculum sp. USNM PAL 772359, reflected light, palladium coated. d: Lateral view of a probable 6-loculed endocarp, apex up; a single intact germination valve in the center, displaying the central lineation that divides it in two. e: Apical view; two bi-partite germination valves are visible, indicated by arrows to the middle cleavage line of two of the valves. f: Basal view, the locules suggested by the undulations in the margin. g–i: Indet. Spondioideae. USNM PAL 772358, reflected light, palladium coated. g: Lateral view of multi-locular endocarp, apex up. h: Apical view showing finely punctuate surface and peripheral locule cavities. i: Basal view. j–m: Cf. Pleiogynium USNM PAL 772357. j: Lateral view of the multi-locular endocarp, apex up; note intact germination valve on left and exposed locule facing the viewer, micro-CT scan surface rendering. k: Lateral view, rotated about 30° from (j), showing three exposed locules, reflected light, palladium coated; note bipartite locule lining at center. l: Apical view of the multilocular endocarp; the locule with intact germination valve at the upper right, reflected light, palladium coated. Arrows to each locule. m: Basal view showing central point of attachment and prominent radiating ridges aligned with the locules, micro-CT scan surface rendering. n–q: Canarium, USNM PAL 772361. Scale bar = 1 cm. n: Lateral view of endocarp directly facing one germination valve flanked by two strong ridges; apex up; specimen coated in sodium nitrate and photographed by R. A. Scott. o: Lateral view facing one of the three pronounced ridges, flanked to the left and right by two germination valves; apex up. p: Apical view displaying the three strong ridges, arching over the apex and flanking three deep embayments, covered with germination valves. q: Basal view, the three ridges being less pronounced. o–q: Reflected light, palladium coated.
Supplementary material 23 from: Hita Garcia F, Fischer G, Liu C, Audisio TL, Economo EP (2017) Next-generation morphological character discovery and evaluation: an X-ray micro-CT enhanced revision of the ant genus Zasphinctus Wheeler (Hymenoptera, Formicidae, Dorylinae) in the Afrotropics. ZooKeys 693: 33-93. https://doi.org/10.3897/zookeys.693.13012
Video 10 : Data type: Video File (.mov)
Figure 1 from: Hita Garcia F, Fischer G, Liu C, Audisio TL, Economo EP (2017) Next-generation morphological character discovery and evaluation: an X-ray micro-CT enhanced revision of the ant genus Zasphinctus Wheeler (Hymenoptera, Formicidae, Dorylinae) in the Afrotropics. ZooKeys 693: 33-93. https://doi.org/10.3897/zookeys.693.13012
Figure 1 - Schematic line drawings illustrating the measurements used in this study. A Body in profile with measuring lines for PH, PTH, and WL B Mesosoma and metasoma in dorsal view with measuring lines for A3L, A3W, A4L, A4W, A5L, A5W, A6L, A6W, DML, PW, PTL, and PTW C Head in full-face view with measuring lines for HL, HW, and SL D Metafemur in dorsal view with measuring line for MFL.
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