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4,276
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ShareScore release 0.9.0
Dataset results
4,276 results for “transcription factors”
The SAS-Chromatin Remodeling Complex Mediates Inflorecence-Specific Chromatin Accessibility for Transcription Factor Binding [ChIP-Seq]
GEO Series GSE254655. Arabidopsis thaliana. 24 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
transcription factor PlagL2 regulates steps in chylomicron metabolism
GEO Series GSE9123. Mus musculus. 8 samples. Type: Expression profiling by array.
Transcription factor-like 5 is a potential DNA/RNA binding protein essential for maintaining male fertility in mice
GEO Series GSE187385. Mus musculus. 3 samples. Type: Expression profiling by high throughput sequencing.
Mesp1 induces a subset of hematopoietic-associated transcription factors in ES cell-derived Flk1+Tie2+ endothelium
GEO Series GSE34537. Mus musculus. 2 samples. Type: Expression profiling by array.
Transcription profiling of human MCF10A cells subjected to ionizing radiation and treatment with transforming growth factor beta-1
Transforming growth factor beta-1 (TGFbeta) is a tumor suppressor during the initial stage of tumorigenesis, but it can switch to a tumor promoter during neoplastic progression. Ionizing radiation (IR), both a carcinogen and a therapeutic agent, induces TGFbeta activation in vivo. We now show that IR sensitizes human mammary epithelial cells (HMEC) to undergo TGFbeta-mediated epithelial to mesenchymal transition (EMT). Non-malignant HMEC (MCF10A, HMT3522 S1 and 184v) were irradiated with 2 Gy shortly after attachment in monolayer culture, or treated with a low concentration of TGFbeta (0.4 ng/ml), or double-treated. All double-treated (IR+TGFbeta) HMEC underwent a morphological shift from cuboidal to spindle-shaped. This phenotype was accompanied by decreased expression of epithelial markers E-cadherin, beta-catenin and ZO-1, remodeling of the actin cytoskeleton, and increased expression of mesenchymal markers N-cadherin, fibronectin and vimentin. Furthermore, double-treatment increased cell motility, promoted invasion and disrupted acinar morphogenesis of cells subsequently plated in Matrigel. Neither radiation nor TGFbeta alone elicited EMT, even though IR increased chronic TGFbeta signaling and activity. Gene expression profiling revealed that double treated cells exhibit a specific 10-gene signature associated with Erk/MAPK signaling. We hypothesized that IR-induced MAPK activation primes non-malignant HMEC to undergo TGFbeta-mediated EMT. Consistent with this, Erk phosphorylation were transiently induced by irradiation, persisted in irradiated cells treated with TGFbeta, and treatment with U0126, a Mek inhibitor, blocked the EMT phenotype. Together, these data demonstrate that the interactions between radiation-induced signaling pathways elicit heritable phenotypes that could contribute to neoplastic progression. Experiment Overall Design: Nonmalignant human mammary epithelial MCF10A cells (passages 106 and 108) were seeded at cloning density in 35mm dishes (10^5 cells/dish). Cell culture medium consisted of 3ml/dish of MGEM serum free medium (Cambrex Inc.), supplemented or not with 400pg/ml recombinant Transforming Growth Factor-beta. Cells were irradiated or not 5h post plating using 160 KV X-ray with a total dose of 2Gy. Sham, IR-treated, TGFbeta-treated and double-treated (IR+TGFbeta) MCF10A cells were harvested 8 days post-IR. Briefly, cells were washed with PBS, denatured in Trizol, scraped off the dish and subjected to chloroform extraction. After centrifugation, the upper phase was precipitated with an equal volume of isopropanol. RNA precipitates were resuspended in RNase free water and further purified on RNeasy columns (Qiagen, Germany). RNA quality was assessed on an Agilent Bio-Analyzer. The dataset analyzed by microarray included biological duplicates for each treatment in two independent experiments and three sham treated samples. Microarray data were generated at the Lawrence Berkeley National Laboratory Molecular Profiling Laboratory (http://hta.lbl.gov) using a high-throughput, automated GeneChip system (Affymetrix). Briefly, target preparation, HT_HG-U133A array plate hybridization setup, washing and staining were performed on an Affymetrix robotic system (GCAS) using version 2.1 protocols. Scanning (protocol version 2.2.09) was performed on a CCD-based high throughput scanner (Affymetrix). Samples were analyzed and clustered with the (UNO) One Color GenetrafficTM software version 3.2-12 (Iobion Informatics LLC, Stratagene, La Jolla, CA). Genes whose expression was specifically altered by treatment were defined as those in which dye ratio was more than 1.75-fold (mean log2ratio>0.8) from baseline in at least three out of the four treated samples compared to the three sham samples. Significance analysis tests (p<0.05) were performed using Excel between sham samples and either IR, TGFbeta or TGFbeta+IR samples.
Transcription factor Etv3 controls the tolerogenic function of dendritic cells (RNA-seq of Etv3 KO GM-DCs)
GEO Series GSE306682. Mus musculus. 5 samples. Type: Expression profiling by high throughput sequencing.
Polymerization of BTB transcription factors regulates chromatin occupancy [RNA-Seq]
GEO Series GSE251638. Homo sapiens. 99 samples. Type: Expression profiling by high throughput sequencing.
Pharmacologic inhibition of BAF chromatin remodeling complexes as a therapeutic approach to transcription factor-dependent cancers [WES]
GEO Series GSE241449. Homo sapiens. 10 samples. Type: Other.
Generation of Mature Megakaryocytes from Pluripotent Stem Cells by a Chemically-Defined Transcription Factor-Based Forward Programming Approach
GEO Series GSE54822. Homo sapiens. 16 samples. Type: Expression profiling by array.
Transcription factor occupancy is affected by genetic variation in the white adipose tissue of C57BL6/j and 129S1/SvImJ mice
GEO Series GSE281688. Mus musculus. 64 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Transcription factor induction of vascular blood stem cell niches in vivo
GEO Series GSE124151. Danio rerio. 35 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other.
Myeloid, B, and T lineage regeneration from pluripotent stem cells by defined transcription factors
GEO Series GSE189736. Mus musculus. 1 samples. Type: Expression profiling by high throughput sequencing.
The histone modification H3K27me3 and transcription factor MoGti1 coordinately control expression of effector genes in rice blast fungus
GEO Series GSE159923. Pyricularia oryzae. 48 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Identification of Transcription Factor EGL-5::POL-2 Binding Regions in L3
GEO Series GSE40945. Caenorhabditis elegans. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Effect of combinatorial transcription factor overexpression on iPSC-derived endothelial cells
GEO Series GSE233694. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
UV irradiation remodels the specificity landscape of transcription factors
GEO Series GSE223023. Arabidopsis thaliana; synthetic construct; Mus musculus; Homo sapiens. 46 samples. Type: Other.
A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1 [G1E_ATACseq]
GEO Series GSE133404. Mus musculus. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Study 5- RNA-seq of male KOLF2.2J hiPSC-derived cortical brain organoids and extra-embryonic lineages homozygous null for nine different transcription factors and one RNA splicing factor
GEO Series GSE287844. Homo sapiens. 48 samples. Type: Expression profiling by high throughput sequencing.
The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis through inhibition of Wnt signaling
GEO Series GSE70300. Mus musculus. 24 samples. Type: Expression profiling by array.
Cell State Dependent Alteration of Melanoma Plasticity and Immunity by the Circadian Transcription Factor Bmal1 [scRNA-Seq]
GEO Series GSE202287. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.