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FIGURES 22–29 in Restoring an old concept of Pholeuonopsis (=Blattodromus syn. nov.) and new recombination for one species from the Balkan Peninsula (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini)

FIGURES 22–29. Pholeuonopsis (s. str.) grabowskii grabowskii Apfelbeck, 1907a, 22–23: aedeagus, dorsal and left latero– ventral aspects, scale bar 0.86 mm; 24: median lobe apex, ventral aspect, scale bar 0.19 mm; 25: paramera apex, dorsal aspect, scale bar 0.02 mm. 26: redrawing of spermatheka of Pholeuonopsis (s. str.) tarensis, from Ćurčić & Pavićević 2018 in S. Ćurčić et al. 2018. 27: redrawing of spermatheka of Pholeuonopsis (s. str.) sljivovicensis, from S. Ćurčić et al. 2015. 28: redrawing of spermatheka of Pholeuonopsis (s. str.) perucensis, from S. Ćurčić et al. 2014. 29: Vilina pećina cave, on the entrance, Lebršnik Mts., Bosnia & Herzegovina.

FIGURES 15–21 in Restoring an old concept of Pholeuonopsis (=Blattodromus syn. nov.) and new recombination for one species from the Balkan Peninsula (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini)

FIGURES 15–21. Pholeuonopsis (s. str.) herculeana Reitter, 1904, 15–16: aedeagus, dorsal and left latero–ventral aspects, scale bar 1.37 mm; 17: median lobe apex, ventral aspect, scale bar 0.44 mm; 18: paramera apex, dorsal aspect, scale bar 0.03 mm; 19: female ventrite VIII, dorsal aspect, scale bar 1.00 mm; 20: style, dorsal aspect, scale bar 0.35 mm; 21: spermatheca, dorsal aspect, scale bar 0.20 mm.

FIGURES 7–14 in Restoring an old concept of Pholeuonopsis (=Blattodromus syn. nov.) and new recombination for one species from the Balkan Peninsula (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini)

FIGURES 7–14. Pholeuonopsis (s. str.) herculeana Reitter, 1904, 7: venter, dorsal aspect, scale bar 0.89 mm; 9: mesoventral carina, right lateral aspect, scale bar 0.23 mm; 11: metendosternite, dorsal aspect, scale bar 1.40 mm; 12: metatergal apparatus, dorsal aspect, scale bar 0.94 mm. Pholeuonopsis (s. str.) grabowskii grabowskii Apfelbeck, 1907a, 8: venter, dorsal aspect, scale bar 0.62 mm; 10: mesoventral carina, right lateral aspect, scale bar 0.20 mm; 13: metendosternite, dorsal aspect, scale bar 0.51 mm; 14: metatergal apparatus, left side and medial part, dorsal aspect, scale bar 0.62 mm.

FIGURES 1–6 in Restoring an old concept of Pholeuonopsis (=Blattodromus syn. nov.) and new recombination for one species from the Balkan Peninsula (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini)

FIGURES 1–6. Pholeuonopsis (s. str.) herculeana Reitter, 1904, 1: habitus, dorsal aspect, scale bar 1.25 mm, original by P. Krásenský, ex Hlaváč et al. 2017: 92; 3: pronotum, dorsal aspect, scale bar 1.88 mm; 5: head, dorsal aspect, scale bar 0.80 mm. Pholeuonopsis (s. str.) grabowskii grabowskii Apfelbeck, 1907a, 2: habitus, dorsal aspect, scale bar 0.97 mm, original by P. Krá- senský, ex Hlaváč et al. 2017: 96; 4: pronotum, dorsal aspect, scale bar 1.20 mm; 6: head, dorsal aspect, scale bar 0.60 mm.

opencc-zeroAug 2021View details →

dryad32/100

Altered 3D chromatin structure permits inversional recombination at the IgH locus

Immunoglobulin heavy chain (IgH) genes are assembled by two sequential DNA rearrangement events that are initiated by recombinase activating gene products (RAG) 1 and 2. Diversity gene segments (DH) rearrange first, followed by variable (VH) gene rearrangements. Here we provide evidence that each rearrangement step is guided by different rules of engagement between rearranging gene segments. DH gene segments, that recombine by deletion of intervening DNA, must be located within a RAG1/2 scanning domain for efficient recombination. In the absence of intergenic control region 1, a regulatory sequence that delineates the RAG scanning domain on WT IgH alleles, VH and DH gene segments can recombine with each other by both deletion and inversion of intervening DNA. We propose that VH gene segments find their targets by diffusion-controlled mechanisms. These distinct mechanisms may underlie differential allelic choice associated with each step of IgH gene assembly.

opencc-by-4.0Sep 2021View details →

Barley recombination open data

Data concerning the publication "Genomic prediction of the recombination rate variation in barley – A route to highly recombinogenic genotypes".

opencc-by-4.0Nov 2022View details →

CX3CR1 CreERT2 TdTomato Fatemap Recombination for brain, meninges, and blood

These files contain quantification of recombination efficiency in tamoxifen-injected CX3CR1 CreERT2 TdTomato mice. The blood data was acquired longitudinally over six weeks, whereas the brain and meninges data were acquired at the end of the six weeks. Workspace files are included to demonstrate gating and quantification.

dryad32/100

Drosophila melanogaster recombination experiments with inversion heterozygotes

Recombination suppression in chromosomal inversion heterozygotes is a well-known but poorly understood phenomenon. Surprisingly, recombination suppression extends far outside of inverted regions where there are no intrinsic barriers to normal chromosome pairing, synapsis, double-strand break formation, or recovery of crossover products. The interference hypothesis of recombination suppression proposes heterozygous inversion breakpoints possess chiasma-like properties such that recombination suppression extends from these breakpoints in a process analogous to crossover interference. This hypothesis is qualitatively consistent with chromosome-wide patterns of recombination suppression extending to both inverted and uninverted regions of the chromosome. The present study generated quantitative predictions for this hypothesis using a probabilistic model of crossover interference with gamma-distributed inter-event distances. These predictions were then tested with experimental genetic data (>40,000 meioses) on crossing-over in intervals that are external and adjacent to four common inversions of Drosophila melanogaster. The crossover interference model accurately predicted the partially suppressed recombination rates in euchromatic intervals outside inverted regions. Furthermore, assuming interference does not extend across centromeres dramatically improved model fit and partially accounted for excess recombination observed in pericentromeric intervals. Finally, inversions with breakpoints closest to the centromere had the greatest excess of recombination in pericentromeric intervals, an observation that is consistent with negative crossover interference previously documented near Drosophila melanogaster centromeres. In conclusion, the experimental data support the interference hypothesis of recombination suppression, validate a mathematical framework for integrating distance-dependent effects of structural heterozygosity on crossover distribution, and highlight the need for improved modeling of crossover interference in pericentromeric regions.

opencc-zeroJan 2023View details →

opencc-by-4.0Feb 2023View details →

Rhometa: Population recombination rate estimation from metagenomic read datasets

Rhometa provides a suite of pipelines for calculating the recombination rate in metagenomic datasets. The tool is developed using Nextflow, a workflow management tool, and the Python programming language. The repository contains all results generated using Rhometa for the manuscript. During the development of Rhometa, various pipelines were employed, including our Nextflow LDhat pipeline. The scripts and results for all pipelines are included. The Github repositories for the pipelines are referenced in the accompanying manuscript. Files named "figure" pertain to the evaluation of simulated data, the simulations themselves have not been included to save space. However, the scripts used to generate the simulations and the final results are included. The file "Lookup_tables.zip" contains lookup tables used in the analysis. The lookup tables are too large to include, but the scripts used to create them are included. Where applicable, experiment accession codes are provided.

dryad32/100

Sorghum leaf blight phenotypes for two recombinant inbred line populations

Sorghum leaf blight and northern corn leaf blight, both caused by Exserohilum turcicum, are major diseases of sorghum and maize, respectively. Examining the genetic architecture of resistance in sorghum will lead to a better understanding of the relationship between resistance in sorghum and maize, which can ultimately enhance management options in both crops. In 2018 and 2019 we evaluated two sorghum recombinant inbred line (RIL) populations for resistance to E. turcicum. The BTx623 x IS3620C and BTx623 x SC155 populations consisted of 235 and 81 RILs, respectively. Resistance in both populations was moderately to highly heritable. We identified a total of six quantitative trait loci (QTL) across the two populations. Three QTL with small to moderate effect sizes were identified in the BTx623 x IS3620C population. Three QTL, including a large-effect QTL on chromosome three that explained 24% of the variation, were identified in the BTx623 x SC155 population. We compared the identified QTL with the position of northern corn leaf blight candidate genes and found eight candidate resistance gene orthologs that colocalize with the sorghum leaf blight QTL. There were also several nucleotide-binding leucine rich repeat encoding genes within the candidate intervals. Understanding host resistance in multiple species furthers our understanding of the Exserohilum turcicum pathosystem.

opencc-zeroApr 2023View details →

Fig. 7 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 7. Antibacterial effects of a series of purified McDef1 concentration against S. aureus (A), E. coli (B), S. pullorum (C) and A. hydrophila (D). 1: 30 μg/ml, 2: 40 μg/ ml, 3: 50 μg/ml, 4: 60 μg/ml, 5: 80 μg/ml, 6: 100 μg/ml, 7: Negative control, empty vector expression supernatant; 8: Positive control, for A̢B̢C: 0.2 mg/mL Ampicillin, for D: 0.5 mg/mL Kanamycin.

Fig. 6 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 6. Antibacterial effects of recombinant yeasts with McDef1 or McDef2 fermentation supernatant on S. aureus (A), S. pullorum (B), E. coli (C) and A. hydrophila (D). 1: Fermentation supernatant of the recombinant McDef1; 2: Fermentation supernatant of the recombinant McDef2; 3: Negative control, empty vector expression supernatant; 4: Positive control, A̢B̢C: 0.2 mg/mL Ampicillin; D: 0.5 mg/mL Kanamycin.

Fig. 4 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 4. Multiple sequence alignments of the mature peptides of McLTP1 homologs from different plants with ClustalW2. The target proteins McLTP1 of this study are highlighted with yellow fluorescent background. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 2. Multiple sequence alignments of the McDef1 (A) and McDef2-5 (B) homologs from different plants with ClustalW2. The conserved domains α-core and γ-core are marked under the homologous sequences respectively. The target proteins McDef1 in (A) and McDef2-5 in (B) of this study are highlighted with yellow fluorescent background. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 1. The alignment analysis of McDef1-5. The underlined amino acids represent the signal sequence predicted with SignalP. The cysteines are marked in yellow background and the putative disulfide bonds are also shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8 in Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins

Fig. 8. Antibacterial effects of a series of purified McDef2 concentration against S. aureus (A), E. coli (B), S. pullorum (C) and A. hydrophila (D). 1: 30 μg/ml, 2: 40 μg/ ml, 3: 50 μg/ml, 4: 60 μg/ml, 5: 80 μg/ml, 6: 100 μg/ml, 7: Negative control, empty vector expression supernatant; 8: Positive control, for A̢B̢C: 0.2 mg/mL Ampicillin, for D: 0.5 mg/mL Kanamycin.

closedIPD-NOFeb 2026View details →

Safety, and Immunogenicity Study of the Recombinant Two-component COVID-19 Vaccine (CHO Cell)

ClinicalTrials.gov study NCT05084989. IPD Sharing: NO. Countries: 1. Publications: 2.

restrictedIPD-UNDECIDEDFeb 2026View details →

Use of Activated Recombinant FVII in Spinal Surgery

ClinicalTrials.gov study NCT00102037. IPD Sharing: Not stated. Countries: 1. Publications: 2.

closedIPD-NOFeb 2026View details →

Study on the Thrombolytic Effect of Platelet Membrane Coated Recombinant Staphylokinase on Human Arterial Thrombus

ClinicalTrials.gov study NCT05978791. IPD Sharing: NO. Countries: 1. Publications: 25.