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Supplementary material 6 from: Li Y, Evans NT, Renshaw MA, Jerde CL, Olds BP, Shogren AJ, Deiner K, Lodge DM, Lamberti GA, Pfrender ME (2018) Estimating fish alpha- and beta-diversity along a small stream with environmental DNA metabarcoding. Metabarcoding and Metagenomics 2: e24262. https://doi.org/10.3897/mbmg.2.24262
PCoA plot based on all the replicates from Eagle Creek :
Supplementary material 7 from: Li Y, Evans NT, Renshaw MA, Jerde CL, Olds BP, Shogren AJ, Deiner K, Lodge DM, Lamberti GA, Pfrender ME (2018) Estimating fish alpha- and beta-diversity along a small stream with environmental DNA metabarcoding. Metabarcoding and Metagenomics 2: e24262. https://doi.org/10.3897/mbmg.2.24262
Profiling table for all the replicates. :
Figure 5b from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 5b The distribution of Hippopotamyrus sp. 'Buzi', (red circle), Hippopotamyrus sp. 'Pungwe' (blue circle) and Hippopotamyrus sp. 'Ruo' (green circle) in the Eastern Zimbabwe Highlands freshwater ecoregion and adjacent areas.
Figure 4a from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 4a Bayesian phylogenetic tree based on mtDNA cytochrome oxidase sub unit I (COI) sequences showing the candidate species or molecular operational taxonomic units (MOTUs) identified within Zaireichthys monomotapa from the Eastern Zimbabwe Highlands freshwater ecoregion. Well supported nodes are shown by a solid circle. The indicated candidate species or MOTUs were identified using the GMYC method based on trees that were built using three different combinations of priors and rates of molecular evolution: (i) yule model and a constant clock, (ii) yule model and a relaxed clock, and (iii) coalescent model with constant population size and a constant clock.
Figure 5a from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 5a Bayesian phylogenetic tree based on mtDNA cytochrome oxidase sub unit I (COI) sequences showing the candidate species or molecular operational taxonomic units (MOTUs) identified within Hippopotamyrus ansorgii from the Eastern Zimbabwe Highlands freshwater ecoregion. Well supported nodes are shown by a solid circle. The indicated candidate species or MOTUs were identified using the GMYC method based on trees that were built using three different combinations of priors and rates of molecular evolution: (i) yule model and a constant clock, (ii) yule model and a relaxed clock, and (iii) coalescent model with constant population size and a constant clock.
Figure 4b from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 4b The distribution of Zaireichthys monomotapa sensu stricto (green circle), Zaireichthys sp. 'slender' (red circle) and Zaireichthys sp. 'leopard spot' (orange circle) in the Eastern Zimbabwe Highlands freshwater ecoregion and adjacent areas.
Figure 2c from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 2c The distribution of Chiloglanis sp. 'dwarf' (green circle), Chiloglanis sp. 'Nyangombe' (blue circle) and Chloglanis sp. 'Pungwe' (red circle) in the Eastern Zimbabwe Highlands freshwater ecoregion and adjacent areas.
Figure 3b from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 3b The distribution of Amphilius sp. 'natalensis Buzi' (dark blue circle), Amphilius sp. 'natalensis Pungwe' (red circle) and Amphilius sp. 'natalensis Ruo' (light blue circle), Amphilius sp. 'uranoscopus Save' (purple triangle), Amphilius sp. 'uranoscopus Buzi' (black triangle), Amphilius sp. 'uranoscopus Pungwe' (green triangle), Amphilius sp. 'uranoscopus Zambezi' (orange triangle) and Amphilius sp. 'uranoscopus Ruo' (yellow triangle) in the Eastern Zimbabwe Highlands freshwater ecoregion and adjacent areas.
Figure 3a from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 3a Bayesian phylogenetic tree based on mtDNA cytochrome oxidase sub unit I (COI) sequences showing the candidate species or molecular operational taxonomic units (MOTUs) identified within Amphilius uranoscopus and A. natalensis from the Eastern Highlands of Zimbabwe. Well supported nodes are shown by a solid circle. The indicated candidate species or OTUs were identified using the GMYC method based on trees that were built using three different combinations of priors and rates of molecular evolution: (i) yule model and a constant clock (YULE), (ii) coalescent model with constant population size and a constant clock (CONC) and (iii) yule model and a relaxed clock (RELA).
Figure 2b from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 2b The distribution of Chiloglanis sp. 'rough skin' (orange circle), Chiloglanis sp. 'Zambezi' (blue circle) and Chloglanis sp. 'Shire' (purple circle) in the Eastern Zimbabwe Highlands freshwater ecoregion and adjacent areas.
Figure 2a from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 2a Bayesian phylogenetic tree based on mtDNA cytochrome oxidase sub unit I (COI) sequences showing the candidate species or molecular operational taxonomic units (MOTUs) identified within Chiloglanis neumanni from the Eastern Zimbabwe Highlands freshwater ecoregion. Well supported nodes are shown by a solid circle. The indicated candidate species or MOTUs were identified using the GMYC method based on trees that were built using three different combinations of priors and rates of molecular evolution: (i) coalescent model with constant population size and a constant clock (CONC), (ii) yule model and a constant clock (YULE) and (iii) yule model and a relaxed clock (RELA).
Figure 1 from: Chakona A, Kadye WT, Bere T, Mazungula DN, Vreven E (2018) Evidence of hidden diversity and taxonomic conflicts in five stream fishes from the Eastern Zimbabwe Highlands freshwater ecoregion. ZooKeys 768: 69-95. https://doi.org/10.3897/zookeys.768.21944
Figure 1 Map of the Eastern Zimbabwe Highlands (EZH) freshwater ecoregion showing the river systems (Lower Zambezi, Pungwe, Buzi and Save) that drain this region and the sampling localities for the present study.
Figure 1 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 1 - Location of the Vermelho River, eastern Atlantic basin, Antonina, Paraná state, Brazil. Black circles indicate the sampling reaches; arrow indicates the flow direction. Source: PEREIRA Jaime Luiz Lopes, 2015.
Figure 4 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 4 - Species rarefaction curves for reaches in the Vermelho River, Paraná state, Brazil. E(Sn) denotes the expected number of species according to abundance.
Figure 3 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 3 - Scatter plot of sampling sites and mesohabitats in the Vermelho River, Paraná state, Brazil, along the environmental gradient produced by the first two PCA axes (PC1 and PC2). Inner figure shows Pearson correlations of variables with PC1.
Figure 6 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 6 - Ordination of sampling mesohabitats in the middle reach through a detrended correspondence analysis (DCA) applied to density matrix of fish species in the Vermelho River, Paraná state, Brazil.
Figure 2 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 2 - Fish sampling procedure. Length (above) and average depth (below) of each blocked (with seines) sampling site are showed. Middle sites were clustered into mesohabitats (see text). Displacement of fishing indicates the upstream direction of passage of electrofishing dip nets with electrodes.
Figure 5 from: Lazzarini Wolff L, Segatti Hahn N (2017) Fish habitat associations along a longitudinal gradient in a preserved coastal Atlantic stream, Brazil. Zoologia 34: 1-13. https://doi.org/10.3897/zoologia.34.12975
Figure 5 - Ordination of the sampling sites through a detrended correspondence analysis (DCA) applied to the density matrix of fish species in the Vermelho River, Paraná state, Brazil.
Figure 1 in Length-weight and length-length relationships of 10 fish species from headwater streams of the lower Iguassu River basin, Brazil
Figure 1. Sampled streams in the Iguassu River basin, Brazil: 1) São José Stream, 2) Lageado Stream, 3) Pedregulho Stream, 4) Rio do Salto Stream, 5) Arroio Passo Liso Stream, 6) Iapu Stream, 7) Três Barras Stream, 8) Aparecida Stream and 9) Caçula Stream. PR, Paraná State; SC, Santa Catarina State; RS, Rio Grande do Sul State.
Table 3. Principal component loading values for seven habitat variables across 13 in Impacts of Wastewater Effluent on Temperate Stream Fish Assemblage Structure
<p>Table 3. Principal component loading values for seven habitat variables across 13 sites.</p><table><tbody><tr><th>Variable</th><th>PC1</th><th>PC2</th><th>PC3</th><th>PC4</th><th>PC5</th><th>PC6</th><th>PC7</th></tr></tbody><tbody><tr><th>Mean depth</th><td>0.48071</td><td>0.28063</td><td>0.17675</td><td>0.44277</td><td>0.31253</td><td>0.60423</td><td>0.01098</td></tr><tr><th>Max depth</th><td>0.52734</td><td>0.2317</td><td>0.01965</td><td>0.21237</td><td>0.15811</td><td><i>–</i> 0.7713</td><td>0.05367</td></tr><tr><th>Discharge</th><td>0.1488</td><td>0.53788</td><td><i>–</i> 0.4311</td><td>0.37747</td><td><i>–</i> 0.599</td><td>0.02831</td><td><i>–</i> 0.0247</td></tr><tr><th>% gravel</th><td>0.52703</td><td><i>–</i> 0.0162</td><td><i>–</i> 0.1975</td><td><i>–</i> 0.5051</td><td><i>–</i> 0.3351</td><td>0.17973</td><td>0.53227</td></tr><tr><th>% pebble</th><td><i>–</i> 0.0551</td><td><i>–</i> 0.5723</td><td><i>–</i> 0.4095</td><td>0.52749</td><td>0.10692</td><td><i>–</i> 0.0208</td><td>0.46008</td></tr><tr><th>% cobble</th><td><i>–</i> 0.292</td><td>0.16769</td><td>0.66603</td><td>0.16685</td><td><i>–</i> 0.1803</td><td><i>–</i> 0.0806</td><td>0.61335</td></tr><tr><th>% boulder</th><td><i>–</i> 0.3204</td><td>0.47152</td><td><i>–</i> 0.3636</td><td><i>–</i> 0.2349</td><td>0.60197</td><td><i>–</i> 0.0034</td><td>0.35384</td></tr></tbody></table>
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