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4,529 results for “drosophila”
Data underlying the publication: Gut bacteria-derived volatiles mediate the Drosophila melanogaster (Diptera: Drosophilidae) attraction
<p>Bacteria-originated volatile molecules play a crucial role in chemical communications between insects, representing their promising application as odor bait traps in pest control. In this study, we investigated the behavioral preferences of the fruit fly <em>Drosophila melanogaster</em> (Diptera: Drosophilidae) towards the fermentation broth of seven gut-associated bacteria using trap choice assays. All seven bacterial fermentations significantly attracted adults and larvae compared to the medium control. We assessed the effects of bacterial fermentations on bayberry fruits' olfaction attractiveness to fly adults and found that the bayberry fruits sprayed with fermentation broth from seven bacteria were all significantly more attractive to insects than the non-sprayed fruits, resulting in increased egg numbers. We also compared the attractive effect of bacterial fermentations with a sugar-vinegar mixture and a commercial odor-bait. The commercial odor bait proved more enticing than the unconcentrated 5-day fermentation broths. However, out of the seven bacteria, 64-fold concentrated bacterial fermentation of <em>Corynebacterium</em> (Actinomycetota phylum) was significantly more attractive than commercial bait. Finally, we chemically identified the predominant compounds 2-methylpropanal and acetaldehyde, which are likely responsible for the behavioral preference of fruit flies. Our findings provide a deeper understanding of how gut microbes affect insect behavior and offer a potential bacteria-originated odor bait for fly control in the orchard.</p>
Data Analysis for "Effects of an Entomopathogenic Fungus on the Reproductive Potential of Drosophila males"
<p>Dataset and code used for data analyses and visualization in our manuscript:</p> <p>"Effects of an Entomopathogenic Fungus on the Reproductive Potential of Drosophila Males"</p> <p>by Aijuan Liao, Fanny Cavigliasso, Loriane Savary and Tadeusz J. Kawecki</p> <p>Published in Ecology and Evolution</p>
Biochemical fractionation of human α-Synuclein in a Drosophila model of synucleinopathies
<p>Synucleinopathies are a group of central nervous system pathologies that are characterized<br>by the intracellular accumulation of misfolded and aggregated α-synuclein in proteinaceous depositions<br>known as Lewy Bodies (LBs). The transition of α-synuclein from its physiological to pathological<br>form has been associated with several post-translational modifications such as phosphorylation and<br>an increasing degree of insolubility, which also correlate with disease progression in post-mortem<br>specimens from human patients. Neuronal expression of α-synuclein in model organisms, including<br>Drosophila melanogaster, has been a typical approach employed to study its physiological effects.<br>Biochemical analysis of α-synuclein solubility via high-speed ultracentrifugation with buffers of<br>increasing detergent strength offers a potent method for identification of α-synuclein biochemical<br>properties and the associated pathology stage. Unfortunately, the development of a robust and<br>reproducible method for the evaluation of human α-synuclein solubility isolated from Drosophila<br>tissues has remained elusive. Here, we tested different detergents for their ability to solubilize<br>human α-synuclein carrying the pathological mutation A53T from the brains of aged flies. We also<br>assessed the effect of sonication on the solubility of human α-synuclein and optimized a protocol<br>to discriminate the relative amounts of soluble/insoluble human α-synuclein from dopaminergic<br>neurons of the Drosophila brain. Our data established that, using a 5% SDS buffer, the three-step<br>protocol separates cytosolic soluble, detergent-soluble and insoluble proteins in three sequential<br>fractions according to their chemical properties. This protocol shows that sonication breaks down<br>α-synuclein insoluble complexes from the fly brain, making them soluble in the SDS buffer and thus<br>enriching the detergent-soluble fraction of the protocol.</p>
Data from: Sensory perception of rivals has trait-dependent effects on plasticity in Drosophila melanogaster
<p>The social environment has myriad effects on individuals, altering reproduction, immune function, cognition, and aging. Phenotypic plasticity enables animals to respond to heterogeneous environments such as the social environment but requires that they assess those environments accurately. It has been suggested that combinations of sensory cues allow animals to respond rapidly and accurately to changeable environments, but it is unclear whether the same sensory inputs are required in all traits that respond to a particular environmental cue. <em>Drosophila melanogaster</em> males, in the presence of rival males, exhibit a consistent behavioral response by extending mating duration. However, exposure to a rival also results in a reduction in their lifespan, a phenomenon interpreted as a trade-off associated with sperm competition strategies. <em>D. melanogaster</em> perceive their rivals by using multiple sensory cues; interfering with at least two of olfactory, auditory, or tactile cues eliminates the extension of mating duration. Here we assessed whether these same cues were implicated in the lifespan reduction. Removal of combinations of auditory and olfactory cues removed the extended mating duration response to a rival, as previously found. However, we found that these manipulations did not alter the reduction in lifespan of males exposed to rivals or induce any changes in activity patterns, grooming or male-male aggression. Therefore, our analysis suggests that lifespan reduction is not a cost associated with the behavioral responses to sperm competition. Moreover, this highlights the trait-specific nature of the mechanisms underlying plasticity in response to the same environmental conditions. </p>
Data from: Numerical data for systemic effects of photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) porphyrin on healthy Drosophila melanogaster
<p>Porphyrins are commonly used as photosensitizing agents (PA) in photodynamic therapy (PDT), a non-invasive method predominantly used for the treatment of subcutaneous tumors. Development of novel PA with increased tissue selectivity and effectiveness is important for the wider use of PDT in the treatment of various types of cancers. We used <em>Drosophila melanogaster</em> as a model organism to study the systemic effects of 5,10,15,20-tetrakis(<em>N</em>-methylpyridinium-3-yl)-porphyrin (TMPyP3). First, we defined the optimal feeding protocol and showed that TMPyP3 was absorbed and retained longer in the neuronal than the non-neuronal extracts. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) concentration increased 24 hours after the photoactivation of orally delivered TMPyP3 but only in the head extracts. After 7 days, regardless of the photoactivation, TMPyP3 led to lower concentration of H<sub>2</sub>O<sub>2</sub>, which correlated with the decreased climbing ability measured as negative geotaxis. The results suggest that systemic treatment with TMPyP3 may interfere with redox regulation and thus impair cellular signaling and behavioral output. Further studies are needed to establish the disruptive effect that porphyrins have on redox homeostasis, how long it persists, and mechanistic differences of retention between different tissues.</p>
Data and code for: A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila (doi: https://doi.org/10.1101/2024.03.28.587162)
<p><strong> </strong>Raw data and R code for:</p> <p>Tiina S. Salminen, Laura Vesala, Yuliya Basikhina, Megan Kutzer, Tea Tuomela, Ryan Lucas, Katy Monteith, Arun Prakash, Tilman Tietz<sup> </sup>and Pedro F. Vale. A naturally occurring mitochondrial genome variant confers broad protection from infection in <em>Drosophila </em>(<strong>doi:</strong> https://doi.org/10.1101/2024.03.28.587162)</p> <p><em> </em></p> <p><strong>Data files:</strong></p> <p>CFU_stats.R: statistics for CFU data</p> <p>Prettgeri_CFU.csv: colony forming units (CFUs) in <em>P. rettgeri</em>-infected flies</p> <p>Saureus_CFU.csv: colony forming units (CFUs) in <em>S. aureus</em>-infected flies</p> <p> </p> <p>gene exp_mitotypes-stats.R: statistics for the qPCR data</p> <p>gene exp_mitotypes.csv: data on the relative gene expression of <em>FucTC</em>, <em>AANATL3</em>, <em>Acp1</em> and <em>CG3397</em> among mitotypes</p> <p> </p> <p>copynumber_stats.R: statistics for the copynumber data</p> <p>copynumber.csv: Mitochondrial copy number as copies of the mtDNA target gene <em>16S</em> relative to nuclear target gene <em>RpL32 </em></p> <p> </p> <p>respirometry_stats.R: statistics for the respirometry data</p> <p>respirometry.csv: Mitochondrial respiration measured in uninfected male flies by measuring oxygen consumption of the OXPHOS complexes I, III and IV</p> <p> </p> <p>ROS_stats.R: statistics for ROS</p> <p>ROS.csv: hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) quantification (whole flies)</p> <p> </p> <p>wasp_stats.R: Statistics on the proportion of melanised and unmelanised wasp larvae</p> <p>Wasps_data.csv: Data on proportion of melanised (killed) and unmelanised (living) <em>L. boulardi</em> parasitoid wasp larvae found in <em>Drosophila</em> larvae</p> <p> </p> <p>CC_stats.R: statistics on crystal cell counts</p> <p>CC.csv: amount of hemocytes called crystal cell in the larvae</p> <p> </p> <p>HC_stats.R: statistics on hemocyte counts</p> <p>hc_uninf.csv: total and activated hemocyte (blood cell) counts in uninfected larvae</p> <p>hc_inf.csv: total and activated hemocyte (blood cell) counts in infected larvae (48 h after infection by <em>L. boulardi</em> parasitoid wasps)</p> <p> </p> <p>PPO3.R: statistics on PPO3 expression levels</p> <p>PPO3.csv: data on gene expression level of prophenoloxidase (PPO3)</p> <p> </p> <p>MMP.R: statistics on MMP</p> <p>cybridMMP.csv: mitochondrial membrane potential (MMP) measured from larval hemocytes using the TMRM dye</p> <p> </p> <p>CellROX.R: statistics on CellROX<sup>TM </sup>signal</p> <p>CellROX.csv: Reactive oxygen species (ROS) measured from larval hemocytes using CellROX<sup>TM</sup> green reagent</p> <p> </p> <p>Hazard.Rmd: hazard ratios</p> <p>Combined.xlsx: data on hazard ratios Hazard ratios of survival post <em>P. rettgeri, S. aureus, </em>Kallithea and DCVinfections</p> <p> </p> <p>Mito_priming.R: Statistics on the effect of priming</p> <p>mito_priming.csv: data on the effect of immune priming with heat-killed bacteria prior to infection</p> <p> </p> <p>hc_stats_supplementary.R: statistics for sex-specific differences in hemocyte counts (data: hc_uninf.csv & hc_inf.csv )</p> <p>PI_hc_stats.R: statistics on dead (Propidium iodide, PI-positive) vs. living (PI-negative) larval hemocytes (data: hc_uninf.csv & hc_inf.csv )</p> <p> </p> <p>melanisation_stats.R: statistics on melanisation response</p> <p>melanisation_males.csv: melanisation response in hemolymph</p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p>
Drosophila pupal retina
<p>Confocal imaging data associated with paper:</p> <p> <a href="https://pubmed.ncbi.nlm.nih.gov/38533736/">Inter-plane feedback coordinates cell morphogenesis and maintains 3D tissue organization in the Drosophila pupal retina. </a></p> <div><span>Sun X, Decker J, Sanchez-Luege N, Rebay I.</span> <span>Development. 2024 Mar 15;151(6):dev201757. doi: 10.1242/dev.201757. Epub 2024 Mar 27.</span> <span>PMID: <span>38533736</span></span></div>
Alternative reproductive tactic in male Drosophila prolongata
<p>Species with intense male-male competition for access to females often show alternative reproductive tactics (ARTs) where males of lower competitive ability adopt a sneaky behavior to gain access to mates. These ARTs are usually associated with intrasexual dimorphisms, in that males with distinct morphologies show different tactics. In some cases, however, males adopt different tactics without being dimorphic. Male <em>Drosophila prolongata</em> exhibit continuous variation in body size and shape, with enlarged forelegs that they use in male-male contests and in courtship, including stimulation of the female's abdomen. During this 'leg vibration', however, nearby males can intercept the courted female and mate without their own courtship. Here, we studied the causes and consequences of these different mating tactics in competitive mating trials between males varying in their size and shape. We found that male mating tactics were condition-dependent. Whereas large, high-condition males were more likely to show leg vibration in their courtship, smaller, lower-condition males were more likely to intercept. However, the number of offspring produced was independent of male condition and reproductive tactic. We discuss possible scenarios for the evolution and maintenance of the ARTs and some future directions for the study of ARTs in this species and in general.</p>
Title of Dataset: NMR metabolomic analysis of Drosophila head extracts: 2 genotypes (control, paraKO)
<p>Characterization of <em>para<sup>ko</sup></em> model in <em>Drosophila melanogaster</em> showed homeostasis disturbances as heat-induced phenotype, neuromuscular and cognitive alterations. Moreover, preliminary results during starvation assay revealed possibly differences in metabolism. To assess that NMR spectroscopy was made, comparing heads from young control and mutant flies.</p>
DNA Metabarcoding Enables High-Throughput Detection of Spotted Wing Drosophila (Drosophila suzukii) within Unsorted Trap Catches
<p>Data to support the paper: DNA Metabarcoding Enables High-Throughput Detection of Spotted Wing Drosophila (Drosophila suzukii) within Unsorted Trap Catches</p>
Impact of modified caneberry trellis systems on microclimate and habitat suitability for Drosophila suzukii
<p>Caneberries are trellised to facilitate harvest and agrochemical applications as well as to improve crop yield and quality. Trellising can also increase airflow and light penetration within the canopy and affect its microclimate. We compared an experimental trellis that split the canopy into halves to standard I- and V-trellises, measuring <i>Drosophila suzukii</i> (Matsumura) fruit infestation as well as canopy temperature and relative humidity in raspberries at two commercial you-pick diversified farms. To evaluate the combined effects of trellising systems and pruning, we pruned one half of each row in blackberry plantings at two research farms and assessed <i>D. suzukii</i> infestation, canopy microclimate (temperature, relative humidity, and light intensity), fruit quality parameters (interior temperature, total soluble solids, and penetration force), and spray coverage/deposition. Trellis installation costs, labor inputs, and yield were used to further evaluate the trellis systems from an economic perspective. Fruit quality was not affected by trellising or pruning and lower total yield was observed in the experimental trellis treatment on one farm. Although <i>D. suzukii</i> infestation was only affected by trellising and pruning at one site, we observed a relationship between higher temperatures and reduced infestation on nearly all farms. Occasionally, lower relative humidity and high light intensity corresponded with lower infestation. Ultimately, the experimental trellis was less economically efficient than other trellising systems and our ability to successfully manipulate habitat favorability varied in a site-specific manner. <i>Drosophila suzukii </i>management approaches that rely upon unfavorable conditions are likely to be more effective in hot, dry regions.</p>
Impacts of male-killing Spiroplasma on the metabolic rate and ectoparasite resistance capacity (endurance) of Drosophila
<p>Ectoparasitic mites are hypothesized to horizontally transmit bacterial endosymbionts, especially male-killing Spiroplasma. In this study we test how <em>Spiroplasma poulsonii </em>MSRO affects fly physiology and behaviour, and potential mite interactions.</p>
Census and phenotype data supporting Drosophila adaptive tracking
<p>Direct observation of evolution in response to natural environmental change can resolve <span>fundamental questions about adaptation including its pace, temporal dynamics, and underlying</span> p<span>henotypic and genomic architecture. </span>W<span>e tracked evolution of fitness-associated phenotypes and</span> <span>allele frequencies genome-wide in ten replicate field populations of <i>Drosophila melanogaster</i></span><i> </i><span>over ten generations from summer to late fall. Adaptation </span><span>was evident over each sampling</span> <span>interval (1-4 generations) with exceptionally rapid phenotypic adaptation and large allele frequency shifts at many independent loci. The direction and basis of the adaptive response</span> <span>shifted repeatedly over time, consistent with the action of strong and rapidly fluctuating</span> <span>selection. Overall, we find clear phenotypic and genomic evidence of a</span><span>daptive tracking</span> <span>occurring contemporaneously with environmental change, demonstrating the temporally dynamic</span> <span>nature of adaptation.</span></p>
Data from: Sperm metabolic rate predicts female mating frequency across Drosophila species
<p>Female mating rates vary widely, even among closely related species, but the reasons for this variation are not fully understood. Across <i>Drosophila </i>species, female mating frequencies are positively associated with sperm length. This association may be due in part to sperm limitation, with longer-spermed species transferring fewer sperm, or to cryptic female choice. However, a previously overlooked factor is sperm metabolic rate, which may correlate with sperm length. If faster-metabolizing sperm accumulate age-related cellular damage more quickly, then females should remate sooner to obtain fresh sperm. Alternatively, frequent female mating may select for increased sperm competitiveness via increased metabolism. Here, we measure sperm metabolism across 13 <i>Drosophila </i>species and compare these measures to published data on female mating rate and on sperm length. Using fluorescent lifetime imaging microscopy, we quantify NAD(P)H metabolism ex vivo, in intact organs. Phylogenetically controlled regression reveals that sperm metabolic rate is positively associated with sperm length and with female mating frequency. Path analysis shows sperm length driving sperm metabolism and sperm metabolism either driving or being driven by female mating rate. While the causal directionality of these relationships remains to be fully resolved, and the effect of sperm metabolism on sperm aging and/or sperm competitiveness remains to be established, our results demonstrate the importance of sperm metabolism in sexual selection.</p>
Data from: Natural Zeitgebers under temperate conditions cannot compensate for the loss of a functional circadian clock in timing of a vital behavior in Drosophila
<p><span>The adaptive significance of adjusting behavioral activities to the right time of the day seems obvious but is under debate. Our data provides evidence that proper timing of eclosion, a vital behavior of the fruit fly <em>Drosophila melanogaster</em>, requires a functional molecular clock under quasi-natural conditions. </span></p> <p><span>We compared eclosion profiles and assessed eclosion rhythmicity in wildtype flies (CS) and clock-related mutant strains <em>(per<sup>01</sup></em>, <em>pdf<sup>01</sup></em>, <em>han<sup>5304</sup></em>) under laboratory and outdoor conditions. In the laboratory, flies were entrained in either light-dark cycle (LD12:12) or warm (25°C)-cold (16°C) cycle (WC12:12), and tested under entrainment or constant conditions using TriKinetics Drosophila Eclosion Monitors. For outdoor assays, a WEclMon system was used and experiments were performed between July-Octobre 2014 and July-Octobre 2016. </span></p> <p><span>Flies with a defective molecular clock showed impaired rhythmicity and gating under natural temperate conditions in Würzburg/Germany even in the presence of a full complement of abiotic Zeitgebers. We also found that eclosion rhythmicity cannot be entrained by daily cycles in relative humidity. Low relative humidity also did not or only weakly affect the ability of the flies to eclose and unfold their wings.</span></p> <p><span>Our results suggest that the presence of natural Zeitgebers is not sufficient, and a functional molecular clock is required to induce stable temporal eclosion patterns in flies under temperate conditions with considerable day-today variation in light intensity and temperature. Temperate Zeitgebers are, however, sufficient to functionally rescue a loss of PDF-signalling.</span></p> <p><span>The data set belongs to the publication:</span></p> <p><em><span>Ruf F, Mitesser O, Mungwa ST, Horn M, Rieger D, Hovestadt T, and Wegener C (2021) Natural Zeitgebers Under Temperate Conditions Cannot Compensate for the Loss of a Functional Circadian Clock in Timing of a Vital Behavior in Drosophila. </span>Journal of Biological Rhythms 36: 271–285. DOI: 10.1177/0748730421998112.</em></p>
Sex-specific variation in R-loop formation in Drosophila melanogaster
<p>Source Data for manuscript, "Sex-specific variation in R-loop formation in <em>Drosophila melanogaster</em>".</p>
Environmental variation and biotic interactions limit adaptation at ecological margins: lessons from rainforest Drosophila and European butterflies
<p>Models of local adaptation to spatially varying selection predict that maximum rates of evolution are determined by the interaction between increased adaptive potential owing to increased genetic variation, and the cost genetic variation brings by reducing population fitness. We discuss existing and new results from our laboratory assays and field transplants of rainforest Drosophila and UK butterflies along environmental gradients, which try to test these predictions in natural populations. Our data suggest that: (i) local adaptation along ecological gradients is not consistently observed in time and space, especially where biotic and abiotic interactions affect both gradient steepness and genetic variation in fitness; (ii) genetic variation in fitness observed in the laboratory is only sometimes visible to selection in the field, suggesting that demographic costs can remain high without increasing adaptive potential; and (iii) antagonistic interactions between species reduce local productivity, especially at ecological margins. Such antagonistic interactions steepen gradients and may increase the cost of adaptation by increasing its dimensionality. However, where biotic interactions do evolve, rapid range expansion can follow. Future research should test how the environmental sensitivity of genotypes determines their ecological exposure, and its effects on genetic variation in fitness, to predict the probability of evolutionary rescue at ecological margins. This article is part of the theme issue 'Species' ranges in the face of changing environments (Part II)'.</p>
Data for: Neural correlates of individual odor preference in Drosophila
<p>Data associated with: Neural correlates of individual odor preference in Drosophila</p> <p>Abstract: Behavior varies even among genetically identical animals raised in the same environment1–10. However, little is known about the circuit or anatomical underpinnings of this individuality11,12. Drosophila olfaction is an ideal system for discovering the origins of behavioral individuality among genetically identical individuals. The fly olfactory circuit is well-characterized13–16,17–19 and stereotyped15, yet stable idiosyncrasies in odor preference4, neural coding4, and neural wiring20,21 are present and may be relevant to behavior. Using paired behavior and two-photon imaging measurements, we show that individual odor preferences in odor-vs-air and odor-vs-odor assays are predicted by idiosyncratic calcium dynamics in Olfactory Receptor Neurons (ORNs) and Projection Neurons (PNs), respectively. This suggests that circuit variation at the sensory periphery determines individual odor preferences. Furthermore, paired behavior and immunohistochemistry measurements reveal that variation in ORN presynaptic density also predicts odor-vs-odor preference. This point in the olfactory circuit appears to be a locus of individuality where microscale variation gives rise to idiosyncratic behavior. To unify these results, we constructed a leaky-integrate-and-fire model of 3,062 neurons in the antennal lobe. In these simulations, stochastic fluctuations at the glomerular level, like those observed in our ORN immunohistochemistry, produce variation in PN calcium responses with the same structure as we observed experimentally, the very structure that predicts idiosyncratic behavior. Thus, our results demonstrate how minute physiological and structural variations in a neural circuit may produce individual behavior, even when genetics and environment are held constant.</p>
No major cost of evolved survivorship in Drosophila melanogaster populations coevolving with Pseudomonas entomophila
<p><span>Rapid exaggeration of host and pathogen traits via arms race dynamics is one possible outcome of host-pathogen coevolution. However, the exaggerated traits are expected to incur costs in terms of resource investment in other life-history traits. The current study investigated the costs associated with evolved traits in a host-pathogen coevolution system. We used the <em>Drosophila melanogaster</em> (host)-<em>Pseudomonas entomophila</em> (pathogen) system to experimentally derive two selection regimes, one where the host and pathogen both coevolved, and the other, where only the host evolved against a non-evolving pathogen. After 17 generations of selection, we found that hosts from both selected populations had better post-infection survivorship than controls. Even though the coevolving populations tended to have better survivorship post-infection, we found no clear evidence that the two selection regimes were significantly different from each other.. There was weak evidence for the coevolving pathogens being more virulent than the ancestral pathogen. We found no major cost of increased post-infection survivorship. The costs were not different between the coevolving hosts and the hosts evolving against a non-evolving pathogen. We found no evolved costs in the coevolving pathogens. Thus, our results suggest that increased host immunity and pathogen virulence may not be costly.</span></p>
No evidence that relatedness or familiarity modulate male harm in Drosophila melanogaster flies from a wild population
<p>Sexual selection frequently promotes the evolution of aggressive behaviours that help males compete against their rivals, but which may harm females and hamper their fitness. Kin selection theory predicts that optimal male-male competition levels can be reduced when competitors are more genetically related to each other than to the population average, contributing to resolve this sexual conflict. Work in <em>Drosophila melanogaster</em> has spearheaded empirical tests of this idea, but studies so far have been conducted in lab-adapted populations in homogeneous rearing environments that may hamper kin recognition, and used highly skewed sex ratios that may fail to reflect average natural conditions. Here, we performed a fully factorial design with the aim of exploring how rearing environment (i.e. familiarity) and relatedness affect male-male aggression, male harassment, and overall male harm levels in flies from a wild population of <em>Drosophila melanogaster</em>, under more natural conditions. Namely, we: a) manipulated relatedness and familiarity so that larvae reared apart were raised in different environments, as is common in the wild, and b) studied the effects of relatedness and familiarity under average levels of male-male competition in the field. We show that, contrary to previous findings, groups of unrelated-unfamiliar males were as likely to fight with each other and harass females than related-familiar males, and that overall levels of male harm to females were similar across treatments. Our results suggest that the role of kin selection in modulating sexual conflict is yet unclear in <em>Drosophila melanogaster</em>, and call for further studies that focus on natural populations and realistic socio-sexual and ecological environments.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.