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zenodo28/100

Figure 29 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 29 Spine of the accessory penial gland, Laspionchis bourkei matangensis. A Holotype, Peninsular Malaysia, [5958 H] (USMMC 00055) B Peninsular Malaysia, [5959] (USMMC 00056) C Philippines, [3343] (PNM 041254) D Peninsular Malaysia, [5960] (USMMC 00056) E same as A F same as B. Scale bars: 100 μm (A–C), 50 μm (D), 5 μm (E, F).

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zenodo28/100

Figure 27 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 27 Live specimens, Laspionchis bourkei matangensis. A Dorsal view, 13 mm long [1785], Indonesia, Sumatra (UMIZ 00113) B dorsal view, 10 mm long [1784], Indonesia, Sumatra (UMIZ 00113) C dorsal view, 11 mm long [1783], Indonesia, Sumatra (UMIZ 00113) D dorsal view, 15 mm long [5961], Peninsular Malaysia, Matang (USMMC 00056) E dorsal view, 25 mm long [3343], Philippines, Bohol (PNM 041254) F holotype, dorsal view, 15 mm long [5958 H], Peninsular Malaysia, Matang (USMMC 00055) G ventral view, 13 mm long [5963], Peninsular Malaysia, Matang (USMMC 00056) H ventral view, 8 mm long [5965], Peninsular Malaysia, Matang (USMMC 00056) I ventral view, same as E.

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zenodo28/100

Figure 28 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 28 Penial hooks, Laspionchis bourkei matangensis, Peninsular Malaysia A, B Holotype [5958 H] (USMMC 00055) C–E [5959] (USMMC 00056) F [5960] (USMMC 00056). Scale bars: 10 μm (A, C), 3 μm (B), 4 μm (D), 5 μm (E), 20 μm (F).

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zenodo28/100

Figure 26 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 26 Habitats, Laspionchis bourkei matangensis. A, B Peninsular Malaysia, Matang, old and open Rhizophora forest of with hard and soft mud, many creeks, and many old logs (st 256, type locality) C Singapore, mud outside mangrove on sun-exposed mudflat (st 7) D Philippines, narrow mangrove on the edge of fish ponds, tall Rhizophora and Avicennia trees, many old logs (st 194) E Indonesia, Sumatra, soft but solid mud, big Avicennia, a few logs, some Nypa on margin, little open space (st 76) F Vietnam, open Avicennia and Rhizophora mangrove with hard mud by a small road and deep mud near water (st 221).

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zenodo28/100

Figure 25 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 25 Radula, Laspionchis bourkei lateriensis, Indonesia, Ambon A–D holotype [6064 H] (UMIZ 00115) E, F [6063] (UMIZ 00116). A Rachidian and innermost lateral teeth B rachidian and innermost lateral teeth C lateral teeth D lateral teeth E lateral teeth (inferior view) F outermost lateral teeth. Scale bars: 10 μm (A, F), 20 μm (B, C), 30 μm (D), 8 μm (E). Abbreviations: bls basal lateral spine hlt hook of a lateral tooth.

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zenodo28/100

Figure 24 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 24 Spine of the accessory penial gland, Laspionchis bourkeiA–D, FL. bourkei bourkei, Australia, Northern Territory E, GL. bourkei lateriensis, Indonesia, Ambon. A Holotype, [1657 H] (NTM P.57615) B [1666] (NTM P.57618) C [1692] (NTM P.57619) D [1673] (NTM P.57618) E holotype, [6064 H] (UMIZ 00115) F same as A G same as E. Scale bars: 100 μm (A–E), 10 μm (F, G).

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zenodo28/100

Figure 23 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 23 Penial hooks, Laspionchis bourkeiA–D Holotype, L. bourkei lateriensis, Indonesia, Ambon [6064 H] (UMIZ 00115) E, FL. bourkei bourkei, Australia, Northern Territory [1692] (NTM P.57619). Scale bars: 40 μm (A, E), 20 μm (B), 5 μm (C, D), 4 μm (F).

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zenodo28/100

Figure 22 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 22 Anterior, male, copulatory apparatus, Laspionchis bourkei. A Holotype, L. bourkei bourkei, Australia, Northern Territory, [1657 H] (NTM P.57615) B holotype, L. bourkei lateriensis, Indonesia, Ambon, [6064 H] (UMIZ 00115) C holotype, L. bourkei matangensis, Peninsular Malaysia, [5958 H] (USMMC 00055). Scale bars: 1 mm (A), 2 mm (B), 0.5 mm (C). Abbreviations: ag accessory penial gland dd deferent duct ms muscular sac ps penial sheath rm retractor muscle v vestibule.

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zenodo28/100

Figure 21 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 21 Posterior, hermaphroditic (female) reproductive system, Laspionchis bourkei. A Holotype, L. bourkei bourkei, Australia, Northern Territory, [1657 H] (NTM P.57615) B holotype, L. bourkei lateriensis, Indonesia, Ambon, [6064 H] (UMIZ 00115) C holotype, L. bourkei matangensis, Peninsular Malaysia, [5958 H] (USMMC 00055). Scale bars: 2 mm (A), 1 mm (B, C). Abbreviations: dd deferent duct fgm female gland mass hg hermaphroditic gland ov oviduct rs receptaculum seminis sp spermatheca.

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zenodo28/100

Figure 11 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 11 External morphology and nervous system, Laspionchis bouchetiA Australia, Queensland [2693] (MTQ) B, C holotype, Australia, Northern Territory [1688 H] (NTM P.57614). A Dorsal, anterior view B ventral, posterior view C nervous system, dorsal view. Scale bars: 2 mm (A), 3 mm (B), 0.5 mm (C). Abbreviations: lcg left cerebral ganglion lpg left pedal ganglion lplg left pleural ganglion mo male opening ot ocular tentacle rcg right cerebral ganglion rplg right pleural ganglion vg visceral ganglion.

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zenodo28/100

Figure 14 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 14 Reproductive system, Laspionchis bouchetiA Brunei [1037] (BDMNH) B, C holotype, Australia, Northern Territory [1688 H] (NTM P.57614). A Posterior, hermaphroditic (female) reproductive system B posterior, hermaphroditic (female) reproductive system C anterior, male, copulatory apparatus. Scale bars: 3 mm (A) 1 mm (B) 2 mm (C). Abbreviations: ag accessory penial gland dd deferent duct dmf distal muscle fibers fgm female gland mass hg hermaphroditic gland ms muscular sac ov oviduct ps penial sheath rm retractor muscle rs receptaculum seminis sp spermatheca v vestibule.

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zenodo28/100

Figure 17 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 17 Habitats, Laspionchis bourkeiA–DL. bourkei bourkei Australia, Northern Territory EL. bourkei lateriensis, Indonesia, Ambon. AAvicennia on sandy mud (st 61, type locality) B same as A C large Sonneratia alba, open forest, soft mud by shore (st 62) DSonneratia, Rhizophora, and Ceriops mangrove (st 66) E mudflat beside a mangrove and a creek (st 128, type locality).

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zenodo28/100

Figure 2 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 2 Phylogenetic relationships within Laspionchis based on concatenated mitochondrial COI and 16S DNA sequences for 80 individuals (including 19 outgroups). Numbers by the branches are the bootstrap values (maximum likelihood analysis, ML) and the posterior probabilities (Bayesian analysis). Only numbers > 60% (ML) and > 0.9 (Bayesian) are indicated. Numbers for each individual correspond to unique identifiers for DNA extraction. All sequences of Laspionchis individuals are new. Sequences of the outgroups are from our previous studies (Dayrat et al. 2011, 2016, 2017, 2018, 2019; Dayrat and Goulding 2017; Goulding et al. 2018a, b, c). Information on specimens can be found in the lists of material examined and in Table 1. The color used for each subspecies is the same as the color used in Figs 3–7.

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zenodo28/100

Figure 10 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 10 Live animals, Laspionchis boucheti, dorsal view. A 39 mm long [5610], Vietnam, Can Gio (ITBZC IM 00017) B 31 mm long [5609], Vietnam, Can Gio (ITBZC IM 00017) C 15 mm long [2578], Australia, Queensland (MTQ) D 16 mm long [1685], Australia, Northern Territory (NTM P.57613).

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zenodo28/100

Figure 1 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 1 Intestinal loops, dorsal view A, B Type I C, D Type II E–H Between types I and II. Small black arrows show the direction of the intestinal transport. The clockwise loop is in blue. The counterclockwise loop is in yellow. The transitional loop (between clockwise and counterclockwise loops) is in green. Red arrows indicate the orientation of the transitional loop. A Type I, with a transitional loop oriented at 3 o'clock, redrawn from Plate (1893: pl. 8, fig. 29) B Type I, with a transitional loop oriented at 3 o'clock, Wallaconchis sinanui (from Goulding et al. 2018: fig. 8D) C Type II, with a transitional loop oriented at 9 o'clock, redrawn from Plate (1893: pl. 8, fig. 31) D Type II, with a transitional loop oriented at 9 o'clock, Paromoionchis tumidus (from Dayrat et al. 2019: fig. 12A) E Between types I and II, with a descending transitional loop at 7 o'clock, holotype, Laspionchis boucheti, Australia, Northern Territory, [1688 H] (NTM P.57614) F Between types I and II, with a descending transitional loop at 6 o'clock, L. boucheti, Australia, Northern Territory, [1681] (NTM P.57612) G Between types I and II, with a descending transitional loop at 6 o'clock, L. boucheti, Vietnam, [5610] (ITBZC IM 00017) H Between types I and II, with a descending transitional loop at 5 o'clock, L. boucheti, Australia, Queensland, [2612] (MTQ). Scale bars: 1 mm (B), 2 mm (D, E), 5 mm (F–H).

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zenodo28/100

Figure 16 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 16 Spine of the accessory penial gland, Laspionchis bouchetiA Australia, Queensland [2693] (MTQ) B, C Brunei [1037] (BDMNH). Scale bars: 100 μm (A, B), 10 μm (C).

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zenodo28/100

Figure 15 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 15 Penial hooks, Laspionchis bouchetiA Brunei [1037] (BDMNH) B–D Vietnam [5609] (ITBZC IM 00017). Scale bars: 20 μm (A), 40 μm (B, C), 10 μm (D).

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zenodo28/100

Figure 13 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 13 Radula, Laspionchis bouchetiA–D Vietnam [5609] (ITBZC IM 00017) E, F holotype, Australia, Northern Territory [1688 H] (NTM P.57614). A Rachidian and innermost lateral teeth B lateral teeth with rachidian teeth C lateral teeth D outermost lateral teeth E rachidian and lateral teeth F lateral teeth. Scale bars: 10 μm (A) 30 μm (B, F) 50 μm (C) 20 μm (D) 40 μm (E). Abbreviations: 1llt first left lateral tooth 1rlt first right lateral tooth 2llt second left lateral tooth 2rlt second right lateral tooth bls basal lateral spine hlt hook of a lateral tooth mc median cusp phe posterior hook extension rt rachidian tooth.

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Figure 19 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 19 Digestive system, dorsal view, Laspionchis bourkeiA–CL. bourkei bourkei, Australia, Northern Territory D Holotype, L. bourkei lateriensis, Indonesia, Ambon E, FL. bourkei matangensis, Peninsular Malaysia. A [1673] (NTM P.57618) B [1666] (NTM P.57618) C [1693] (NTM P.57619) D [6064 H] (UMIZ 00115) E [5959] (USMMC 00056) F holotype, [5958 H] (USMMC 00055). Scale bars: 2 mm (A, C–E), 2.5 mm (B), 1 mm (F). Abbreviations: ddg dorsal digestive gland i intestine pdg posterior digestive gland r rectum st stomach.

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Figure 18 from: Dayrat B, Goulding TC, Khalil M, Comendador J, Ngô Xuân Q, Tan SK, Tan SH (2019) A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae). ZooKeys 877: 31-80. https://doi.org/10.3897/zookeys.877.36698

Figure 18 Live animals, Laspionchis bourkeiA–D, G, HL. bourkei bourkei Australia, Northern Territory E, F, IL. bourkei lateriensis, Indonesia, Ambon. A Dorsal view, 20 mm long [1618] (NTM P.57617) B dorsal view, 21 mm long [1656] (NTM P.57617) C holotype, dorsal view, 23 mm long [1657 H] (NTM P.57615) D dorsal view, 19 mm long [1673] (NTM P.57618) E dorsal view, 12 mm long [2743] (UMIZ 00116) F dorsal view, 17 mm long [2753] (UMIZ 00116) G ventral view, 19 mm long [1659] (NTM P.57618) H ventral view, 23 mm long [1617] (NTM P.57617) I ventral view, same as E.

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