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1,237 results for “AC”
Figure 2 in Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration
Figure 2. Purification and identification of rAc-cathB-1. (A) Purification of rAc-cathB-1 from the cell culture supernatant. Lanes: MW, molecular weight markers (listed in kDa on the side); 1, concentrated culture supernatant in binding buffer; 2, column flowthrough; 3, binding buffer wash; 4–6, successive eluate fractions (during the elution step, the eluate was collected in one 5 ml tube after another); 7, eluate from gel filtration. (B) Identification of purified rAc-cathB-1 by western blot with an antiserum against Ac-cathB-1 expressed in E. coli and an anti-Myc antibody.
Figure 5 in Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration
Figure 5. Effects of antiserum on the hydrolytic activity of activated rAc-cathB-1 and larval gut penetration. (A) The inhibition of activated rAc-cathB-1 by antiserum. One microgram of rAc-cathB-1 was activated and incubated with the positive serum (6.0 µg) or negative serum (6.0 µg) for 30 min, respectively, prior to assessment of the degradation of Z-RR-AMC. The hydrolytic reaction was performed at 37 °C for 30 min and the fluorescence of released AMC was measured. All data were presented as relative activities of activated rAc-cathB-1, where the activity of the control (without serum treatment) was taken as 100%. (B) Inhibition of larval penetration ability. Two hundred L3 larvae were pretreated with the undiluted positive serum, the undiluted negative serum, or PBS at 37 °C for 30 min, respectively. The three groups of larvae were separately injected into lumens of rat gut sacks and kept in sterilized Tyrode's solution at 37 °C for 3 h. Each trial was conducted in triplicate and the numbers of larvae remaining in the gut lumen were counted. Numbers of L3 that penetrated the isolated gut were calculated and presented as indicated. Asterisk (*), P <0.05; ns, not significant.
Figure 4 in Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration
Figure 4. Assessment of the hydrolytic activity of activated rAc-cathB-1. (A) Activated rAc-cathB-1 completely degraded fibronectin and laminin but did not cleave type I collagen over a 12-h period. In the incubation buffer (pH 6.0), 10 µg of the respective substrates was treated with equal volumes of PBS, activated rAc-cathB-1, or activated rAc-cathB-1 plus E64 at 37 °C for 12 h. After incubation, all samples were analyzed on a 5% SDS-PAGE gel followed by Coomassie brilliant blue staining. Molecular weight markers (in kDa) are listed on the side. The substrate used for each experiment is indicated at the top of the gel, and the presence or absence of a recombinant protease or protease inhibitor is indicated at the bottom of each lane. (B) Influence of rAc-cathB-1 on IEC-6 monolayer. IEC-6 cells were grown to confluence and equal amounts of rAc-cathB-1 with or without E64 were applied to cells for 2 h. The blank was made of IEC-6 with PBS added. After incubation, the adherent IEC-6 partly rounded up and the integrity of the cell sheet was disrupted by activated rAc-cathB-1. The cytoplasm and ECM were then labeled with an anti-laminin antibody (green), and the nucleus was stained with DAPI. On the merged images, the dark regions represent the intercellular space. (C) Statistical analysis of the changes to the intercellular space of IEC-6 cells. The dark area was measured and analyzed. The difference between the means for each group of samples was estimated using one-way ANOVA followed by Duncan's multiple comparison test. Asterisk (*), P <0.05; WL, white light; ns: not significant; and bar = 100 µm.
Figure 3 in Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration
Figure 3. Activation and pH-dependence profile of rAc-cathB-1. (A) Processing of rAc-cathB-1. Purified rAc-cathB-1 (0.2 mg/mL) was incubated with activation solution or reference solution in a 2:1 (v/v) ratio. Mixtures were incubated at 37 °C for 30 min, and the reaction was stopped by addition of pepstatin A (Sigma-Aldrich) to a final concentration of 1 mM. Samples were analyzed on a 12% SDS-PAGE gel followed by Coomassie brilliant blue staining. MW, molecular weight markers; purified rAc-cathB-1 incubated with activation solution and reference solution as indicated. (B) Enzymatic activity assay. Z-Arg-Arg-7-amido-4-methylcoumarin hydrochloride was used for studying the activity of pepsin-treated rAc-cathB-1 and the control. The fluorescence was measured with excitation and emission wavelengths of 355 and 460 nm, respectively, and data were presented as relative activities, where activity of the control was taken as 1. (C) The pH-dependence profile of activated rAc-cathB-1. The assay was performed with the fluorescent substrate at a final concentration of 50 µM. Fluorescence was measured and data were presented as relative activities of activated rAc-cathB-1, where the highest activity at the pH optimum was taken as 100%. Asterisk (*), P <0.05.
◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord in Hardly Venus's servant-morphological adaptations of Veneriserva to an endoparasitic lifestyle and its phylogenetic position within Dorvilleidae (Annelida)
◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord
ESA SEOM-IAS – Measurement and ACS database SO2 UV region
<p>The database contains measurements and absorption cross sections generated within the framework of the ESA project SEOM-IAS (Scientific Exploitation of Operational Missions - Improved Atmospheric Spectroscopy Databases), ESA/AO/1-7566/13/I-BG. Details on the project can be found at http://www.wdc.dlr.de/seom-ias/.</p> <p>The measurements were recorded at the German Aersopace Center (DLR) to provide a new absorption cross section database for SO2 according to the needs of the TROPOMI instrument aboard the Sentinel 5-P satellite. The data are compiled in two zip files, one for the measurements (SO2_UV_region_measurement_database_20112018.zip), one for the absorption cross sections (SO2_UV_region_absorption_cross_section_database_20112018.zip) and a readme file (ESA_SEOM_IAS_spectra_SO2UVRegion_readme.docx).</p>
Supplementary dataset for journal article "AC Characteristics of Low Ohmic Foil Shunts Influenced by Eddy Currents in the Mounting Body"
<p>This is the complete simulation environment for all simulations and plots done for the paper 'AC Characteristics of Low Ohmic Foil Shunts Influenced by Eddy Currents in the Mounting Body'.</p> <p>The entire simulation is based on fully free software:</p> <ul> <li>Linux</li> <li>GNU Octave</li> <li>Fast Field Solver: Fast Henry</li> </ul> <p>All scripts and models have been copied together in one zip-archive.</p>
Non-Abelian anyon statistics through AC conductance of a Majorana interferometer
<p>We provide the raw data used to produce Fig.3 and Fig.4 of our paper "Non-Abelian anyon statistics through AC conductance of a Majorana interferometer". We also provide a s<span>hematic three-dimensional view of the consid</span><span>ered experimental device for observing non-Abelian braiding </span><span>of Ising anyons via the AC conductance.</span></p>
RAW DATA - Control of Intermolecular Interactions toward the Production of Free-Standing Interfacial Polydopamine Films - ACS Applied Materials & Interfaces 2023 15 (30), 36922-36935 DOI: 10.1021/acsami.3c05236
<div>This repository contains RAW data for the experiment described in the publication:</div> <div> </div> <div>Jakub Szewczyk, Visnja Babacic, Adam Krysztofik, Olena Ivashchenko, Mikołaj Pochylski, Robert Pietrzak, Jacek Gapiński, Bartłomiej Graczykowski, Mikhael Bechelany, and Emerson Coy, Control of Intermolecular Interactions toward the Production of Free-Standing Interfacial Polydopamine Films, ACS Applied Materials & Interfaces 2023 <em>15</em> (30), 36922-36935, DOI: 10.1021/acsami.3c05236.</div> <div> </div> <div>For more information please contact the corresponding authors.</div>
Text-fig. 6. Molars of Microtus from Mikhailovka-5. Microtus ex gr. agrestis LINNAEUS, 1761: a–l: M2, m–s: M3; Microtus (Terricola) ex gr. subterraneus (SELYS-LONGCHAMPS, 1836): t–z: m1, aa–ab: m2, ac–ag: M3. in Late Pleistocene (Eemian) Mollusk And Small Mammal Fauna From Mikhailovka-5 (Kursk Oblast, Central Russia)
Text-fig. 6. Molars of Microtus from Mikhailovka-5. Microtus ex gr. agrestis LINNAEUS, 1761: a–l: M2, m–s: M3; Microtus (Terricola) ex gr. subterraneus (SELYS-LONGCHAMPS, 1836): t–z: m1, aa–ab: m2, ac–ag: M3.
Series AC Arc Fault Detection Method Based on High-Frequency Coupling Sensor and Convolution Neural Network
<p>The data provided can be used for the development of methods for the detection of arcing faults in a domestic low-voltage electrical networks (230V - 50 Hz). The data files are current and voltage signatures experimentally measured.</p> <p>Test for to produce an arcing fault : Open contact electrodes and Carbonized path wires</p> <p>The ReadMe file describes :</p> <p>- the test set up and the the procedure followed to make the measurements</p> <p>- the list of household appliances and their main characteristics.</p> <p>- the name of the data files</p> <p>- the type of arcing faults</p>
Arc fault detection and appliances classification in AC home electrical networks using Recurrence Quantification Plots and Image Analysis
<p>The data provided can be used for the development of methods for the detection of arcing faults in a domestic low-voltage electrical networks (230V - 50 Hz). The data files are current and voltage signatures experimentally measured.</p> <p>The ReadMe file describes :</p> <p>- the test set up and the the procedure followed to make the measurements</p> <p>- the list of household appliances and their main characteristics.</p> <p>- the name of the data files</p> <p>- the type of arcing faults</p> <p> </p>
AC&C/SPARC Ozone Database for CMIP5 - Hitop Version
<p>The AC&C/SPARC ozone is provided on pressure levels between 1000-1hPa. For use in high-top models, an extrapolation of the dataset up to ~0.01 hPa or higher, may be required. The UK National Centre for Atmospheric Science (NCAS) has produced an updated/extended version of the SPARC ozone dataset. The following were done: (1) A multiple-linear regression is performed on the historical raw pressure-level data between 1000-1 hPa consistent with the Randal and Wu method used to construct the timeseries. The ozone is then represented as: O3(t) = a*SOL + b*EESC + seasonal_cycle + residuals. For consistency, the 11-yr solar cycle and EESC indices are identical to those used to prepare the original dataset. NOTE: the SOLAR index is a 180.5nm timeseries provided by Fei Wu at NCAR. (2) The seasonal cycle and residual fields are extrapolated and reduced above 1 hPa using O3(z)=O3(1hPa)*exp(-(z-z(1hPa))/H). Where H = 7 km. The seasonal cycle field is then used to provide a smooth climatology of ozone up to the model top. It has also been smoothed in latitude to the poles with a cosine function. (3) To minimize the 11-yr solar cycle and trend components in the mesosphere the a and b regression coefficients are extrapolated and reduced above 1 hPa twice as rapidly as the seasonal cycle, i.e. using the same method but with H = 3.5 km. (4) The full ozone timeseries is then reconstructed using the equation from (1). The ozone climatologies are not affected. (5) The standard SPARC ozone dataset which extends into the future does not include solar cycle variability post-2009. For production of a dataset extending into the future including an 11-yr ozone solar cycle, the solar regression index is used to build a future time series consistent with a repeating solar irradiance compiled by the UK Met Office and is modelled as a sinusoid with a period of 11 years, with mean and max-min values corresponding to solar cycle 23 (Gareth Jones, pers. comm.).</p>
Open Dataset for publication: Resonant laser ionization and mass separation of Ac-225
<p>The dataset for publication in preparation:</p> <p>Resonant laser ionization and mass separation of Ac-225.</p> <p> </p> <p>Includes all data analysed for results given in the publication.</p>
AC efficiency of GrInHy2.0 electrolysis plant (incl. compressor)
<p>AC efficiency [%] of the GrInHy2.0 electrolysis system incl. compressor unit (compression to ~10 bar), with respect to lower heating value of produced hydrogen: eta_{el,plant} = m\dot * H_i / (P_{el,HTE}+P_{el,Compr}), heat intake not included as energy input.</p> <p>Time: May 2021 - October 2022</p>
AC efficiency of GrInHy2.0 HTE electrolyzer
<p>AC efficiency [%] of the SOEC electrolyzer system w/o compressor unit, with respect to lower heating value of produced hydrogen: eta_{el,HTE} = m\dot * H_i / P_{el,HTE}, heat intake not included as energy input.</p> <p>Time: May 2021 - October 2022</p>
Codes to analyze AC plastcity 2P data
<p>Custom written Matlab codes to extract and analyze 2photon data from Tiff raw files. Instructions to run the code is provided in the word file.</p>
Analysis of Effective Stiffness and Anisotropy of AC 16 Asphalt Mixture within NCN project Weave-UNISONO 2021, project No 2021/03/Y/ST8/00079
<p><strong>Summary</strong>:</p> <p>The internal structure of the AC 16 (asphalt concrete mixture) was divided into the mortar phase and the mineral aggregate phase. Static creep tests using the Bending Beam Rheometer were conducted for the mortar phase to fit the rheological model. The aggregate arrangement and orientation were analysed using ImageJ software for the mineral phase. The Finite Element Method (FEM using ABACUS software) meshes were prepared based on images with an assumption of plane strain in 2D formulation. Using the FEM model, the tension/compression tests using selected characteristic directions were conducted, and the effective constrained stiffness moduli were estimated.</p> <p><strong>The dataset includes:</strong></p> <ul> <li>TIFF input and output image of AC16 lateral surface, txt output results file <ul> <li>xz_AC_16 lateral surface_areas colour.tiff</li> <li>xz_AC_16 lateral surface.tiff</li> <li>xz_AC_16 lateral surface ImageJ - results.txt</li> </ul> </li> <li>grey TIFF image for plot profile <ul> <li>grey image for plot profile.tif</li> </ul> </li> <li>BBR test results, CSV raw data <ul> <li>sample 1 mortar.csv</li> <li>sample 2 mortar.csv</li> </ul> </li> <li>Input images: scan in xy plane and scan in xz plane <ul> <li>xy_AC_16 mel-dol.tif</li> <li>xy_AC_16.tif</li> <li>xy_AC_16 ImageJ - results.txt</li> <li>xz_AC_16 mel_dol.tif</li> <li>xz_AC_16.tif</li> <li>xz_AC_16 ImageJ - results.txt</li> </ul> </li> <li>Abaqus Input Files – horizontal and vertical tension <ul> <li>xy_AC_16_horizontal_tension.txt</li> <li>xy_AC_16_vertical_tension.txt</li> <li>xz_yz_AC_16_horizontal_tension.txt</li> <li>xz_yz_AC_16_vertical_tension.txt</li> </ul> </li> </ul>
MINI-AC motif mappings of Arabidopsis and maize
<p>MINI-AC motif mappings of Arabidopsis and maize.</p> <p>For each species, there are the genome-wide motif mappings and the locus-based motif mappings using a non-coding genomic space of 5kb upstream of the translation start site, introns, and 1kb downstream of the translation end site.</p> <p>In version 2 I added, for the locus-based on maize (<em>Zea mays</em>), the motif mappings using the non-coding genomic spaces of: (1) 1kb upstream of the translation start site, introns, and 1kb downstream of the translation end site and (2) 15kb upstream of the translation start site, introns, and 2.5kb downstream of the translation end site.</p> <p>In version 2.5 I added the bed files with the coordinates of the small (1kb upstream of the translation start site, the 1kb downstream of the translation end site, and the introns) and large (15kb upstream of the translation start site, the 2.5kb downstream of the translation end site, and the introns) non-coding genomic spaces.</p> <p>In version 3 I added for maize genome version 5 (B73 RefGen_v5), the bed files with the motif mappings using the non-coding genomic spaces of: (1) 1kb upstream of the translation start site, introns, and 1kb downstream of the translation end site and (2) 5kb upstream of the translation start site, introns, and 1kb downstream of the translation end site, and (3) 15kb upstream of the translation start site, introns, and 2.5kb downstream of the translation end site. Additionally, I added the bed files with the coordinates of the small (1kb upstream of the translation start site, the 1kb downstream of the translation end site, and the introns) and large (15kb upstream of the translation start site, the 2.5kb downstream of the translation end site, and the introns) non-coding genomic spaces.</p>
Telemedicine in High-Risk Cardiovascular Patients Post-ACS
ClinicalTrials.gov study NCT05015634. IPD Sharing: NO. Countries: 1. Publications: 9.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
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OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.