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Figs 18–21 in Nebela kivuense Gauthier-Lièvre et Thomas, 1961 (Amoebozoa, Arcellinida), Missing for a Half-century; Found 11,500 km from "home"

Figs 18–21. Dried tests with circular or elliptical scales "scavenged" from other species of testate amoebae. Figs 18, 20 – light microscope images of tests mounted in Canada Balsam. Figs 19, 21 – scanning electron microscope images of two different tests.

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Figs 2–17 in Nebela kivuense Gauthier-Lièvre et Thomas, 1961 (Amoebozoa, Arcellinida), Missing for a Half-century; Found 11,500 km from "home"

Figs 2–17. Diagramatic representations and light and electron microscope images illustrating test morphology of Nebela kivuense. Figs 2–6, 12a, 13a and 14 – variations in test shape as seen in plan views together with the lateral view of the same tests (Figs 7–11, 12b, 13b). Fig. 15 – scanning electron microscope image showing the flattened anterior region of a test with its thickened rim of organic cement around the pseudostomal aperture (arrow). Fig. 16 – well developed internal cyst plate (arrow). Fig. 17 – thick-walled spherical cyst (arrow).

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Fig. 1. A in Nebela kivuense Gauthier-Lièvre et Thomas, 1961 (Amoebozoa, Arcellinida), Missing for a Half-century; Found 11,500 km from "home"

Fig. 1. A reproduction of Figs 1e and 1f from Gauthier-Lièvre and Thomas (1961) illustrating the shape of the test of Nebela kivuense; a and c – lateral (edge) views; b and d – plan views.

Figs 22–27 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 22–27. Ultrastructure of the amoeba of Luapeleamoeba hula n. g. n. sp. 22. General ultrastructure showing nucleus (N) with homogeneous central nucleolus, mitochondria (M), and centrosomal region with Golgi n. g. n. sp. (G) and MTOC. Note lack of an obvious cell coat. Scale bar – 2.0 µm. 23. Detail of nucleus (N) and portion of the nucleolus (Nu). Scale bar – 1.0 µm. 24. Detail of mitochondrion (M). Scale bar – 500 nm. 25. Detail of cell surface showing microfilament-rich cortex and lack of cell coat. Scale bar – 500 nm. 26, 27. Detail centrosomal region, Golgi (G), MTOC, and microtubules (MT on figures). Fig. 26 is an enlargement of the centrosomal region of Fig. 22 and views the MTOC from a perspective that shows its lamellate structure. Scale bars – 200 nm.

Figs 12–21 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 12–21. Time series of events in Luapeleamoeba hula n. g. n. sp. life cycle. 12–15. Changes in spore shape. 16–18. Ingestion of basidiospore. 19–21. Cytokinesis. All images taken with 20 × dry lens bright field microscopy on agar surface in primary isolation plate (PIP). Approximately 30 sec elapsed between images. Scale bars: 10µm throughout.

Fig. 11 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Fig. 11. Fluorescence image of fixed Luapeleamoeba hula n. g. n. sp. amoebae. Red is actin stain and blue is DNA stain. The amoebae on the left and right are motile, the one in the center is stationary. Small areas of DNA fluorescence outside the nuclei are from undigested bacteria in the food vacuoles. Scale bar 10 µm.

Figs 1–10 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 1–10. Light micrographs of Luapeleamoeba hula n. g. n. sp. strain LHI05M-5a-1. 1. Side view of fruiting body on native substrate in primary isolation plate (PIP). 2. Living amoeba on agar surface in monoeukaryotic culture. The cell is moving in the direction of the bottom of the image. 3. Amoebae and fruiting bodies in various stages of development on agar surface viewed subaerially from top in a PIP during the late afternoon. i – immature sporocarp, M – mature sporocarp, P – prespore cell. Note the apparent thickness of the amoebae. 4. Fruiting bodies in various stages of development viewed subaerially on native substrate in PIP during late afternoon. Amoebae seen obliquely are obviously dome shaped. 5. Stalk with apophysis viewed from side after spore has discharged. 6. Floating form of a living amoeba in liquid media slightly flattened with cover slip under 63 × oil differential interference contrast microscopy (DIC). 7. Amoeba gently fixed on slide to maintain locomotive form, 40 × dry DIC. 8. Two living amoebae in PIP digesting fungal spores. 9. Amoeba gently fixed on slide to maintain locomotive form showing blunted triangular subpseudopodia extending from broad hyaline lamellipodium, 40 × dry DIC. 10. Three amoebae gently fixed on slide to maintain locomotive form 40 × dry phase contrast. Scale bars: 10 µm throughout.

Fig. 1 in A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA

Fig. 1. Photomicrographs of Flamella piscinae n. sp. A – locomotive form with trailing filaments; B – locomotive form; C – locomotive form with subpseudopodia; D – locomotive form; E – cytokinesis; F – floating form; G – floating form; H – single cyst; I – cyst enveloped within multiple walls; J – cysts sharing walls; K – cysts sharing walls in a linear conformation. Scale bar: 10 µm. All images are to scale.

Fig. 7 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 7. Histograms depicting distribution of data for characters analyzed in this study. The black lines represent density curves that fit our data. Aperture height (ah), test diameter (td) and test top diameter (ttd) present a distribution similar to a bimodal distribution, while all the other characters show a nearly normal distribution. Characters tb2 and bw2 are not shown since they are equal to tb1 and bw1, respectively.

Fig. 2 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 2. Lateral view of six individuals of Arcella gandalfi. A and B – individuals with highest shell, top invagination is easily seen; C and D – individuals with intermediate shell height, top invagination easily seen; E and F – individuals with lowest shell height. Scale bar: 20 µm.

Fig. 1 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 1. Representation of measured characters. A – Arcella gandalfi in lateral view, showing aperture height (ah), test height (th), test top invagination (tti) and test top diameter (ttd). B – Arcella gandalfi in apertural view, showing the test diameter (td), aperture diameter (ad), test border 1–2 (tb1–2) and brim width 1–2 (bw1–2).

Fig. 6 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 6. Morphology of Arcella gandalfi under Scanning Electron Microscopy (SEM). A and B – lateral view showing the aperture region; C – shell detail showing elongated shape of the alveolar units on the conical extension of the shell. Scale bars: 20 µm.

Fig. 5 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 5. Representative images of an Arcella brasiliensis individual. A – apertural view, showing the distinct marginal ring (test brim); B – lateral view showing the rounded dome. Scale bar: 20 µm.

Fig. 4 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 4. Apertural view of two individuals of Arcella gandalfi. A and B – distinct marginal ring (test brim) similar to Arcella brasiliensis easily seen. Scale bar: 20 µm.

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Fig. 2 in Molecular Phylogenetics Evidence for a Novel Lineage of Amoebae Within Discosea (Amoebozoa: Lobosa)

Fig. 2. Maximum-Likelihood SSU tree of subphylum Lobosa, with emphasis on major representatives of the class Discosea. The monophyletic resolution of Flabellinia and Longamoebia was obtained after omitting unstable taxa Stygamoeba and Vermistella (see Fig. 1). Members of the class Tubulinea were used as outgroup. Bootstrap values (BV) for ML/NJ/MP were presented at nodes; filled circles – 100% BV with all methods; * – node supported but BV <40%. For acc. nos. – see Fig. 1.

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Fig. 1 in Molecular Phylogenetics Evidence for a Novel Lineage of Amoebae Within Discosea (Amoebozoa: Lobosa)

Fig. 1. Maximum-Likelihood tree based on SSU rDNA of major representatives of the subphylum Lobosa and the class Discosea, following the classification of Smirnov et al. (2011). Members of the class Tubulinea were used as outgroup. Subclasses and orders were indicated, and for Dermamoebida families also. Bootstrap values (BV) for ML/NJ/MP were presented at nodes; filled circles – 100% BV with all methods; * – node supported but BV <40%.

Fig. 7 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa

Fig. 7. Sequential phase contrast micrographs of encysting T. tubasferens. The individual was observed at irregular intervals. A–H – formation of a first, larger cyst; I–J – formation of second, smaller cyst; phase contrast in I, Hoffmann modulation contrast in J. Scale bar for all images: 20 µm.

Fig. 4 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa

Fig. 4. Fine structure of amoeboid forms. A – a nucleus (N) with undulating margin and somewhat irregularly shaped nucleolus (Nu); B – mitochondria (M) with tubular cristae, and the uncoated peripheral plasma membrane with villous-like protrusions (arrow); C – an apparent feeding invagination of the cell contains bacteria (B) and an enlarged arrangement of plasma membrane protrusions (asterisk); D – "trumpet-shaped" tapered assembly of microtubules (arrow) distributed in the cytoplasm; E – an enlarged view of the microtubular assembly showing arrangement of the electron dense broadened attachment plates (arrow) where the microtubules terminate. Scale bar in A: 2 µm, B: 0.5 µm, C: 1 µm and D, E: 0.3 µm.

Fig. 3 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa

Fig. 3. Phase contrast micrographs of T. tubasferens, large locomotive forms. A – a large multinucleated individual in active locomotion; B – a detail of the fine, filose pseudopods at the trailing region of the individual in A; C – a distinct large individual in active locomotion; D – a detail of the anterior hyaline margin of the individual in C; E – a different morphology of large individual, at beginning of locomotion; F – a typical trailing pseudopod at the uroidal region, with corkscrew aspect; G – an individual with a very high number of filose pseudopods protruded at the anterior region; H, I – a locomotive individual with a high number of pseudopods at the trailing region; J, K, L, M – a sequential documentation of a locomotive amoeba, to demonstrate the aspect of the trailing and anterior regions during active movement. Scale bar for all images: 50 µm.

Fig. 2 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa

Fig. 2. Phase contrast micrographs of T. tubasferens trophic forms. A – contrasting size of five individuals within a monoeukaryotic culture; B – a small tetranucleated individual; C – medium sized individual with a large anterior region; D, E, F – different morphologies of small sized individuals; G, H, I – different morphologies of large, multinucleated individuals, without extensive branching; J, K – typical extensively branched large individual, showing many gaps within the cytoplasm; L – a large individual. Scale bar for all images: 50 µm.