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28 results for “Catharanthus roseus”
Fig. 8 in P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata
Fig. 8. Molecular models of Camptotheca CYP72A564, CYP72A565 and CYP72A730. (A) Backbone overlays of Camptotheca CYP72A730, CYP72A564 and CYP72A565 models are shown with the RMSD variance of CYP72A564 and CYP72A565 from the CYP72A730 backbone depicted in green (0.0 Å), yellow (3.0 Å) and red (4.5 Å). (B) SRS regions in CYP72A proteins shown with predicted substrate contact residues (gray fill). (C) Identical versus (D) different side chain residues predicted within 4.5 Å of loganic acid (gray) docked in Camptotheca CYP72A564 (orange) versus loganic acid (aqua) docked in CYP72A730 (magenta). (E) Identical versus (F) different side chain residues predicted within 4.5 Å of loganic acid (gray) docked in Camptotheca CYP72A565 (rose) versus loganic acid (aqua) docked in CYP72A730 (magenta).
Fig. 4 in P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata
Fig. 4. LC-MS analyses of in vitro assays with purified His6-tagged CYP72A proteins reconstituted with His6-tagged CPR proteins. Reactions containing purified His6- tagged CYP72A protein, full-length His6-tagged Caa CPR1 protein (A, B) or full-length His6-tagged Caa CPR2 protein (C, D), and 250 μM loganic acid (A,C) or loganin (B,D), were incubated at 30◦ C and analyzed by LC-MS as described in experimental procedures. Extracted ion chromatograms for loganic acid (m/z 375.1297), secologanic acid (m/z 373.1140), secoxyloganic acid (m/z 389.1089); loganin sodium salt (m/z +413.1418), secologanin sodium salt (m/z +411.1262), secoxyloganin (m/z 403.1246) are given with stacked chromatograms as marked.
Fig. 3 in P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata
Fig. 3. LC-MS analyses of in vitro assays with CYP72A proteins expressed in yeast microsomes. 100 μl reactions containing 10 μl CYP72A microsomes isolated from WAT11 with 250 μM loganic acid or loganin and 500 μM NADPH in 100 mM NaPO (pH 7.4) were incubated at 30◦ C and analyzed by LC-MS as 4 described in the experimental procedures. (A) Extracted ion chromatograms for loganic acid (m/z 375.1297), secologanic acid (m/z 373.1140), secoxyloganic acid (m/z 389.1089). (B) Extracted ion chromatograms for loganin sodium salt (m/z +413.1418), secologanin sodium salt (m/z +411.1262), secoxyloganin (m/z 403.1246).
Fig. 6. Type I in P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminata
Fig. 6. Type I binding spectra of SLAS candidates. Substrate-induced Type I binding spectra for (A) CYP72A564, (B) CYP72A565 and from 998 nM (violet) to 1.90 mM (crimson) using loganic acid (top inset) or loganin (bottom inset). (C) Binding isotherms calculated as the difference of the valley (~419 nm) from the peak (~388 nm) are shown for loganic acid () and loganin (). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Data for: Single-cell multi-omics in the medicinal plant Catharanthus roseus
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Small RNA profiling of aster yellows infected Catharanthus roseus plants
GEO Series GSE213754. Catharanthus roseus. 9 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus
GEO Series GSE217852. Catharanthus roseus. 35 samples. Type: Expression profiling by high throughput sequencing.
Fig. 6. The V19H four-point mutant retained V19H activity and gained T3O-like activity. Recombinant yeast expressing different V19H in Site directed mutagenesis of Catharanthus roseus (+)-vincadifformine 19-hydroxylase (CYP71BY3) results in two distinct enzymatic functions
Fig. 6. The V19H four-point mutant retained V19H activity and gained T3O-like activity. Recombinant yeast expressing different V19H mutants were incubated with (+)-vincadifformine (15) (A) or with ()-tabersonine (1) (B) and reaction products were submitted to UPLV-MS analysis. Traces from yeast expressing the V19H single mutants (orange), from top to bottom, are V19H-L106R, V19H–S312T, V19H-A376P, and V19H–F377L. Traces from yeast expressing the V19H double mutant (pink) is V19H- A376P–F377L, and the 4-point mutant (red) is V19HL106R–S312T-A376P–F377L. Yeast expressing wildtype V19H (green) and wild-type T3O (blue) were used as positive controls for (+)-minovincinine (15) (A) and tabersonine-2,3-epoxide (5) (B) biosynthesis. All V19H mutants retain V19H activity, producing (+)-minovincinine (16) from (+)-vincadifformine (15) (A: orange, pink, and red), but the V19H 4-point mutant gained T3O-like activity, producing tabersonine-2,3-epoxide (5) from ()-tabersonine (1) (B: red). V19H: (+)-vincadifformine 19-hydroxylase; T3O: tabersonine 3-oxygenase. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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