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opencc-zeroApr 2024View details →

Data from: Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin

Pharmacological activation of hypoxia-inducible factor 1alpha (HIF-1α), a hypoxia-responsive transcription factor, has attracted increasing attention due to its efficacy not only in renal anemia but also in various disease models. Our study demonstrated that a HIF-1 activator enhanced exosome production from cultured endothelial cells synergistically with adiponectin, an adipocyte-derived factor, through both transcriptional induction and posttranscriptional stabilization of an adiponectin binding partner, T-cadherin. Increased exosome levels were observed in wild-type mice but not in T-cadherin null mice after consecutive administration of roxadustat. Adiponectin- and T-cadherin-dependent increased exosome production may be involved in the pleiotropic effects of HIF-1 activators.

opencc-by-4.0Feb 2022View details →

Raw Data for the article: Extracellular Vesicle-Derived microRNAs of Human Wharton's Jelly Mesenchymal Stromal Cells May Activate Endogenous VEGF-A to Promote Angiogenesis

Despite low levels of vascular endothelial growth factor (VEGF)-A, the secretome of human Wharton's jelly (WJ) mesenchymal stromal cells (MSCs) effectively promoted proangiogenic responses in vitro, which were impaired upon the depletion of small (~140 nm) extracellular vesicles (EVs). The isolated EVs shared the low VEGF-A profile of the secretome and expressed five microRNAs, which were upregulated compared to fetal dermal MSC-derived EVs. These upregulated microRNAs exclusively targeted the VEGF-A gene within 54 Gene Ontology (GO) biological processes, 18 of which are associated with angiogenesis. Moreover, 15 microRNAs of WJ-MSC-derived EVs were highly expressed (Ct value ≤ 26) and exclusively targeted the thrombospondin 1 (THBS1) gene within 75 GO biological processes, 30 of which are associated with the regulation of tissue repair. The relationship between predicted microRNA target genes and WJ-MSC-derived EVs was shown by treating human umbilical-vein endothelial cells (HUVECs) with appropriate doses of EVs. The exposure of HUVECs to EVs for 72 h significantly enhanced the release of VEGF-A and THBS1 protein expression compared to untreated control cells. Finally, WJ-MSC-derived EVs stimulated in vitro tube formation along with the migration and proliferation of HUVECs. Our findings can contribute to a better understanding of the molecular mechanisms underlying the proangiogenic responses induced by human umbilical cord-derived MSCs, suggesting a key regulatory role for microRNAs delivered by EVs.

opencc-by-4.0Jul 2022View details →

Raw datasets and media accompanying the manuscript: Extracellular Vesicles (EVs) Are Copurified with Feline Calicivirus, yet EV-Enriched Fractions Remain Infectious

Raw datasets and media accompanying the manuscript: Extracellular Vesicles (EVs) Are Copurified with Feline Calicivirus, yet EV-Enriched Fractions Remain Infectious

opencc-by-4.0Jul 2022View details →

Brain-Derived Neurotrophic Factor and extracellular vesicle-derived miRNAs in an Italian cohort of individuals with obesity: a key to explain the link between depression and atherothrombosis.

This record contains raw data related to the article “Brain-Derived Neurotrophic Factor and Extracellular Vesicle-Derived miRNAs in an Italian Cohort of Individuals With Obesity: A Key to Explain the Link Between Depression and Atherothrombosis”  Abstract:  Background: Obesity and depression are intertwined diseases often associated with an increased risk of cardiovascular (CV) complications. Brain-Derived Neurotrophic Factor (BDNF), altered in the brain both of subjects with depression and obesity, provides a potential link between depression and thrombosis. Since the relationship among peripheral BDNF, depression and obesity is not well-defined, the aim of the present report has been to address this issue taking advantage of the contribution played by extracellular vesicle (EV)-derived miRNAs. Research Process: Associations among circulating BDNF, depression and EVderived miRNAs related to atherothrombosis have been evaluated in a large Italian cohort of obese individuals (n = 743), characterized by the Beck Depression Inventory (BDI-II) score. Results: BDI-II was negatively associated with BDNF levels without a significant impact of the rs6265 BDNF polymorphism; this association was modified by raised levels of IFN-g. BDNF levels were linked to an increase of 80 EV-derived miRNAs and a decrease of 59 miRNAs related to atherosclerosis and thrombosis. Network analysis identified at least 18 genes targeted by these miRNAs, 7 of which involved in depression and CV risk. The observation of a possible link among BDNF, depression, and miRNAs related to atherothrombosis and depression in obesity is novel and may lead to a wider use of BDNF as a CV risk biomarker in this specific subject group.

opencc-by-4.0Oct 2022View details →

Bacteria-Derived Extracellular Vesicle Microbiome in NAFLD Patients With and Without Obstructive Sleep Apnea

These are the supplementary figures.

opencc-by-4.0Jul 2024View details →

The effect of extracellular vesicles derived from oral squamous cell carcinoma on the metabolic profile of oral fibroblasts

Oral cancer is one of the most common forms of head and neck cancers. Oral squamous cell carcinoma (OSCC) accounts for more than 90% of the oral malignancies. The molecular pathogenesis of OSCC is complex as it involves altered expression of specific genes and proteins, but also comprises changes in metabolic processes. It is suggested that extracellular vesicles (EVs) released by cancer cells may contribute to cancer development and metastasis by recruiting and changing phenotype of normal cells that surround the tumor. The aim of the project was to characterize the effect of OSCC EVs on the metabolic profile of normal oral fibroblasts (NOFs). Targeted liquid chromatography-mass spectrometry metabolic profiling was performed on control cells and NOFs exposed to OSCC EVs for 24 and 48 h. Analysis of detected metabolites revealed that OSCC EVs affected NOFs the most after 24 h of exposure. Among metabolites that were significantly altered at 24 h, pyruvate, ATP, UTP, coenzyme A, and dihydroxyacetone phosphate were upregulated, while fatty acids such as nervonic acid, linoleate, oleate, palmitoleic acid, and docosahexaenoic acid were downregulated. These findings were supported by Western blotting of pyruvate kinase M2 (PKM2). The metabolic pathways of glycolysis, citric acid cycle, and amino acid metabolism were enriched, suggesting that OSCC EVs cause phenotype switch in NOFs that may contribute to acquiring a pro-tumorigenic phenotype.

opencc-by-4.0Aug 2024View details →

Raw data for: Orthogonal analysis reveals inconsistencies in cargo loading of extracellular vesicles

Raw datasets and media accompanying the manuscript: Orthogonal analysis reveals inconsistencies in cargo loading of extracellular vesicles, published in Journal of Extracellular Biology in 2024.

opencc-zeroAug 2021View details →

Data from: Identifying extracellular vesicles from single cells

Extracellular vesicles (EVs) are constantly secreted from both eukaryotic and prokaryotic cells. EVs, including those referred to as exosomes, may have an impact on cell signaling and an incidence in diseased cells. In this manuscript, a platform to capture, quantify, and phenotypically classify the EVs secreted from single cells is introduced. Microfluidic chambers of about 300 pL are employed to trap and isolate individual cells. The EVs secreted within these chambers are then captured by surface-immobilized monoclonal antibodies (mAbs), irrespectively of their intracellular origin. Immunostaining against both plasma-membrane and cytosolic proteins was combined with highly sensitive, multi-color total internal reflection fluorescence microscopy (TIRFM) to characterize the immobilized vesicles. The data analysis of high-resolution images allowed the assignment of each detected EV to one of 15 unique populations, and demonstrated the presence of highly-heterogeneous phenotypes even at the single-cell level. The analysis also revealed that each mAb isolates phenotypically-different EVs, and more vesicles were effectively immobilized when CD63 was targeted instead of CD81. Finally, we demonstrate how an heterogeneous suppression in the secreted vesicles is obtained when the enzyme neutral sphingomyelinase is inhibited.

opencc-by-4.0Aug 2021View details →

Tunable Resistive Pulse Sensing data of "The impact of storage on extracellular vesicles: a systematic study" experiments

Raw data of Tunable Resistive Pulse Sensing (TRPS) of "The impact of storage on extracellular vesicles: a systematic study" experiments

opencc-by-4.0Aug 2021View details →

Flow cytometry data of "The impact of storage on extracellular vesicles: a systematic study" experiments

Flow cytometry raw data acquired for the pubblication of "The impact of storage on extracellular vesicles: a systematic study"

opencc-zeroAug 2021View details →

Raw data and media: Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers

Raw datasets and media accompanying the manuscript: Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers, published in the Journal of Nanobiotechnology

opencc-zeroOct 2021View details →

Reassessment of the proteomic composition and function of extracellular vesicles in the seminal plasma

Seminal plasma contains a high concentration of extracellular vesicles (EVs). The heterogeneity of small EVs or the presence of non-vesicular extracellular matter(NV) pose major obstacles in understanding the composition and function of seminal EVs. In this study, we employed high-resolution density gradient fractionation to accurately characterize the composition and function of seminal EVs and NV. We found that the seminal EVs could be divided into three different subtypes, namely high-density EV (EV-H), medium-density EV (EV-M), and low-density EV (EV-L) after purification using iodixanol,while NV was successfully isolated. EVs and NV display different features in size, shape and expression of some classic exosome markers. Both EV-H and NV could markedly promote sperm motility and capacitation compared with EV-M and EV-L, whereas only the NV fraction induced sperm acrosome reaction. Proteomic analysis results showed that EV-H, EV-M, EV-L, and NV had different protein components and were involved in different physiological functions. Further study showed that EV-M might reduce the production of sperm intrinsic reactive oxygen species (ROS) through Glutathione S-transferase Mu 2 (GSTM2).This study provides novel insights into important aspects of seminal EVs constituents and sounder footing to explore their functional properties in male fertility.

opencc-zeroMay 2023View details →

The cellular response to extracellular vesicles is dependent on their cell source and dose

Extracellular vesicles (EV) have been established to play important roles in cell-cell communication and have shown promise as therapeutic agents. However, we still lack a basic understanding of how cells respond upon exposure to EVs from different cell sources at various doses. Thus, we treated fibroblasts with EVs from twelve different cell sources at doses between 20 and 200,000 per cell, analyzed their transcriptional effects, and functionally confirmed the findings in various cell types in vitro, and in vivo using single-cell RNA-sequencing. Unbiased global analysis revealed EV dose to have a more significant effect than cell source, such that high doses downregulated exocytosis and upregulated lysozomal activity. However, EV cell source-specific responses were observed at low doses, and these reflected the activities of the EV's source cells. Finally, we assessed EV-derived transcript abundance and found that immune cell-derived EVs were most associated with recipient cells. Together, this study provides important insight into the cellular response to EVs.

controlledIPD-YESFeb 2026View details →

Efficacy of Platelet- and Extracellular Vesicle-rich Plasma in Chronic Postsurgical Temporal Bone Inflammations

ClinicalTrials.gov study NCT04281901. IPD Sharing: YES. Countries: 1. Publications: 2.

closedIPD-NOFeb 2026View details →

Extracellular Vesicle Infusion Treatment for COVID-19 Associated ARDS

ClinicalTrials.gov study NCT04493242. IPD Sharing: NO. Countries: 1. Publications: 1.

closedIPD-NOFeb 2026View details →

Antiplatelet Therapy Effect on Extracellular Vesicles in Acute Myocardial Infarction

ClinicalTrials.gov study NCT02931045. IPD Sharing: NO. Countries: 2. Publications: 1.

restrictedIPD-UNDECIDEDFeb 2026View details →

Fish Oil-derived N-3 Polyunsaturated Fatty Acids and Extracellular Vesicles

ClinicalTrials.gov study NCT03203512. IPD Sharing: Not stated. Countries: 1. Publications: 1.

controlledIPD-YESFeb 2026View details →

Safety Evaluation of Intracoronary Infusion of Extracellular Vesicles in Patients Following Coronary Stent Implantation

ClinicalTrials.gov study NCT04327635. IPD Sharing: YES. Countries: 1. Publications: 1.

publicOct 2021View details →

Reassessment of the proteomic composition and function of extracellular vesicles in the seminal plasma

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