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469 results for “Image Analysis”
Accompanying dataset for: "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues"
<p>Mouse datasets were acquired using the manual IBEX multiplex imaging protocol and accompany the manuscript “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, A. Radtke <em>et al.</em>, 2020, PNAS.</p> <p>All image data are stored using the <a href="https://imaris.oxinst.com/support/imaris-file-format">Imaris file format</a>. To view these multi-channel images, you can either use one of these free viewers, <a href="https://imaris.oxinst.com/imaris-viewer">Imaris viewer</a>, <a href="https://imagej.net/Fiji">Fiji</a>.</p> <p>Each experiment has an associated imaging meta-data file in xlsx format and the resulting image in Imaris format.</p> <p><strong>Mouse spleen (Manual)</strong></p> <p>Dataset is a 16 parameter IBEX experiment performed on a mouse spleen section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse thymus (Manual)</strong></p> <p>Dataset is a 26 parameter IBEX experiment performed on a mouse thymus section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse lung (Manual)</strong></p> <p>Dataset is a 23 parameter IBEX experiment performed on a mouse lung section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.379 µm), y (0.379 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse small intestine (Manual)</strong></p> <p>Dataset is a 20 parameter IBEX experiment performed on a mouse small intestine section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse liver (Manual)</strong></p> <p>Dataset is an 18 parameter IBEX experiment performed on a liver section from a LysM-tdtomato reporter mouse labeled with antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse naive lymph node (Manual)</strong></p> <p>Dataset is a 41 parameter IBEX experiment performed on a mouse lymph node section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse immunized lymph node (Manual)</strong></p> <p>Dataset is a 41 parameter IBEX experiment performed on a mouse lymph node section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p>
Dead Sea Scrolls data collection (images, labels, prediction plots) for dating ancient manuscripts using radiocarbon and AI-based writing style analysis
<p>The dataset is associated with the following article:<br>Title: <strong>Dating ancient manuscripts using radiocarbon and AI-based writing style analysis</strong><br>Authors: Mladen Popović, Maruf A. Dhali, Lambert Schomaker, Johannes van der Plicht, Kaare Lund Rasmussen, Jacopo La Nasa, Ilaria Degano, Maria Perla Colombini, and Eibert Tigchelaar<br><em>(Under review)</em></p> <p>This data set is collected for the ERC project:<br>The Hands that Wrote the Bible: Digital Palaeography and Scribal Culture of the Dead Sea Scrolls<br>PI: Mladen Popović<br>Grant agreement ID: 640497<br>Project website: <a href="https://cordis.europa.eu/project/id/640497">https://cordis.europa.eu/project/id/640497</a></p> <p> </p> <p><strong>Copyright (c) </strong> University of Groningen, 2024. All rights reserved.<br><strong>Disclaimer and copyright notice for all data contained on the *.tar.gz files:</strong></p> <p><strong>1)</strong> permission is hereby granted to use the data for research purposes. It is not allowed to distribute this data for commercial purposes.</p> <p><strong>2) </strong>provider gives no express or implied warranty of any kind, and any implied warranties of merchantability and fitness for purpose are disclaimed.</p> <p><strong>3) </strong>provider shall not be liable for any direct, indirect, special, incidental, or consequential damages arising out of any use of this data.</p> <p><strong>4) </strong>the user should refer to the first public article mentioned above on this data set.</p> <p><strong>5) </strong>the recipient should refrain from proliferating the data set to third parties external to his/her local research group. Please refer interested researchers to this site to obtain their own copy.</p> <p> </p> <p><strong>Organization of the data:<br></strong><em>(Update on 19 April 2024: OxCal data for accepted 2-sigma ranges are updated with the incusion and exclusion of minor peaks. New prediction plots are added after the model is trained with accepted 2-sigma ranges, including minor peaks. The old plots are also kept. <br><br><OLD Updates below; disregard><br>updated on 07-Feb-2024: OxCal data for selected ranges added in a new directory in addition to previously available original OxCal data. Enoch's prediction plots and test images are reorganized for easy access to the users.<br><OLD Updates above; disregard><br><br>Please use the files from this version and disregard the previous two versions: 10.5281/zenodo.10629480 and 10.5281/zenodo.8168210)</em></p> <p>There are four *.tar.gz files:</p> <p><em><strong>C14-Oxcal-data-updated.tar.gz</strong></em> contains one directory with radiocarbon data (OxCal [1] raw data) for all 30 manuscripts. Three additional directories contain name-corrected files for original OxCal data, files with accepted ranges, and files with accepted ranges including minor peaks. Please refer to the original article for details about OxCal data and the manuscripts. 25 out of 30 raw OxCal data are used (accepted ranges only) as the training labels during the training of Enoch, the date prediction model.</p> <p><em><strong>train-images-c14.tar.gz</strong></em> contains the clean and preprocessed (binarized, aligned, and arrangement corrected) training images for the 25 radiocarbon-dated training manuscripts (including 4Q52; 64 images in total). </p> <p><em><strong>test-images-all.tar.gz</strong></em> contains the clean and preprocessed test images for 135 previously undated manuscripts. The images are organized in three different directories: the first one with all 359 images for the 135 manuscripts, the second one with the selected 135 images, and the final one with 25 images to illustrate the poor quality of images. </p> <p><em><strong>Enoch-prediction-new-with-minor-peaks.tar.gz</strong></em> contains the new date prediction plots for each of the 135 test images, where Enoch was trained with the inclusion of minor peaks for the 2-sigma accepted ranges and with a data balancing threshold of 0.05. These plots are used by expert palaeographers' evaluation of Enoch's style-based date predictions of 135 previously undated manuscripts.</p> <p><em><strong>Enoch-predictions.tar.gz</strong></em> contains the date prediction plots for each of the 135 test images. There are two directories inside the *.tar.gz file:<br><br>- <em>prediction-plots-for-selected-135:</em> Prediction plots with data balancing threshold of 0.05. <br>- <em>extra-plots:</em> contains four additional directories:<br> - <em>Enoch-predictions-c14wo4Q52-balanced05:</em> Prediction plots with data balancing threshold of 0.05. <br> - <em>Enoch-predictions-c14wo4Q52-balanced10:</em> Prediction plots with data balancing threshold of 0.1.<br> - <em>Enoch-predictions-c14wo4Q52-unbalanced:</em> Unbalanced raw predictions.<br> - <em>Enoch-predictions-c14wo4Q52-combined:</em> Combined plots with all three prediction plots (unbalanced, 0.05, 0.1).<br>Please refer to the original article for more details.</p> <p>The updated code to run the plot is available here: <a href="https://doi.org/10.5281/zenodo.10998860">https://doi.org/10.5281/zenodo.10998860</a></p> <p><strong>If you have any questions, please get in touch with us:</strong><br>Mladen Popović <m.popovic(at)rug.nl><br>Maruf A. Dhali <m.a.dhali(at)rug.nl><br>Lambert Schomaker <l.r.b.schomaker(at)rug.nl></p> <p> </p> <p><strong>References:</strong><br>1. Bronk Ramsey, C. (2001). Development of the radiocarbon calibration program. <em>Radiocarbon</em>, <em>43</em>(2A), 355-363.</p>
Volumetric and morphological analysis of the clades based on the in vivo confetti imaging
<p>This dataset contains a script in programming language that describes the analytical pipeline for image processing of the in vivo data from the Confetti mice skin. The algorithm describes volumetric analysis, 3D reconstruction, density analysis, as well multiple other morphological parameters. </p>
Reference data and analysis software for "Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane"
<p>Reference data set for the single molecule co-tracking analysis presented in "Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane". Corresponding author for further inquiries:</p> <p>Prof. Dr. Jacob Piehler</p> <p>University of Osnabrück, Department of Biology/Chemistry, Division of Biophysics, Barbarastr. 11, 49076 Osnabrück, Germany</p> <p>https://www.biophysik.uni-osnabrueck.de/</p>
How to Build an Image Processing Pipeline for Automating Multiparameter Histocytometry Analysis
<p>Image files for evaluation of an upcoming Current Protocols submission, as well as associated reference files.</p>
Live cell microscopy: From image to insight - raw data & analysis
<p>Accompanying raw and processed data as well as analysis scripts for the publication Biophysics Rev. 3, (2022); <a href="https://doi.org/10.1063/5.0082799">10.1063/5.0082799</a> "Live cell microscopy: From image to insight".</p>
Comprehensive Automatic Processing and Analysis of Adaptive Optics Flood Illumination Retinal Images
<p>A collaborative research group has established this database to support AO-FIO image utilization and evaluation of photoreceptor detection. <br> Please cite the following publication when using the database:</p> <p>Eva Valterova, Jan D. Unterlauft, Mike Francke, Toralf Kirsten, Radim Kolar, and Franziska G. Rauscher, "Comprehensive automatic processing and analysis of adaptive optics flood illumination retinal images on healthy subjects," Biomed. Opt. Express <strong>14</strong>, 945-970 (2023)<br> <br> The database can be utilized in connection with our application MATADOR for AO-FIO image registration and analysis, which is freely available on:</p> <p>https://github.com/evavalterova/MATADOR.git</p> <p>The database includes</p> <ul> <li>over 200 flood illumination adaptive optics images of 10 normal healthy subjects. Each folder includes 10 images of the right eye (denoted by OD) and 10 images of the left eye (denoted by OS) with their preliminary determined retinal position during image acquisition.</li> <li>foveal and peripheral patches. Each consists of 40 cropped regions from the set of 200 images. In each cropped region are manually labeled positions of photoreceptors by three evaluators.</li> <li>axial lengths of 10 subjects in ".xlsx" file<br> </li> </ul>
Animal Recognition Using Methods Of Fine-Grained Visual Analysis - Kashtanka Pets (All Dev and Test Images, Single Folder)
<p>Kashtanka Pets images, with all Dev and Test images (total 66639 images). In a single folder, with filenames indicating path of file in original dataset distribution.</p>
Image data for bioRxiv article named: mtFociCounter - Reproducible, open source and quantitative single-cell analysis of mitochondrial nucleoids and other foci
<p>Raw imaging data to reproduce and test the findings of the bioRxiv article: <strong>mtFociCounter </strong>- Reproducible, open source and quantitative single-cell analysis of mitochondrial nucleoids and other foci. It contains data from three imaging days and 2 or three technical replicates on each day.</p> <p> </p>
Sample 3D image data from RIMS method for image analysis code demo
<p>Sample 3D image data from RIMS method applied to mechanical test on hydrogel sphere packings, to be used in image analysis code demo as demonstrated in the ALERT Geomechanics doctoral school 2022. The data is a small subset from a larger set of data as found on Dryad via 10.5061/dryad.6djh9w0x8 and is separated here on Zenodo to make the subset more machine-readable.</p>
Database that contains all images (plus 180 more) employed in the article: "Image features for quality analysis of thick blood smears employed in malaria diagnosis"
<p>We share with you a bank of images obtained from microscopic fields of thick blood smears employed in the malaria diagnosis, and also the .csv file that contains the labels for each image.</p> <p>The images are saved with a unique name that is found in the first column of the .csv file. The second column contains the labels from each image, according to their unique names.</p> <p>The labeling process was done with the online toolbox Labelbox. Labelbox, "Labelbox," Online, 2020. [Online]. Available: https://labelbox.com </p> <p>If you are interested in using our database, cite our article as a way to recognize our work. We will be grateful for that. </p> <p>CITATION: Fong Amaris, W.M., Martinez, C., Cortés-Cortés, L.J. et al. Image features for quality analysis of thick blood smears employed in malaria diagnosis. Malar J 21, 74 (2022). https://doi.org/10.1186/s12936-022-04064-2</p> <p>URL of our paper: https://malariajournal.biomedcentral.com/articles/10.1186/s12936-022-04064-2</p> <p><strong>--- This is the link where you can find our images Bank: https://drive.google.com/drive/folders/1Qrv0e4bSEtkeqtPABz-klQp-6D6OjU-X?usp=sharing </strong></p> <p>It is important you to know that along with this .txt file, we are sharing the .csv file that contains 600 names of images (in the first column) with their respective labels (second column aside)</p> <p>This file corresponds to the instructions of an extended label file related to 600 images (and 600 new labels) in contrast to our previous label file with 420 labels from 420 images (https://www.researchgate.net/publication/359439520_Database420LabelsInstructionstxt ; https://www.researchgate.net/publication/359438904_Database420Labelscsv).</p> <p>Best Regards</p> <p> </p>
Automated Segmentation of Large Image Datasets using Artificial Intelligence for Microstructure Characterisation and Damage Analysis
<p>Many properties of commonly used materials are driven by their microstructure, which can be influenced<br>by the composition and manufacturing processes. To optimise future materials, understanding the<br>microstructure is critically important. Here, we present two novel approaches based on artificial intelligence<br>that allow the segmentation of the phases of a microstructure for which simple numerical approaches, such<br>as thresholding, are not applicable: One is based on the nnU-Net neural network, and the other on generative<br>adversarial networks (GAN).<br>Using scanning electron microscopy images collected from large areas (~1 mm²) of dual-phase steels as a<br>case study, we demonstrate how both methods effectively segment intricate microstructural details,<br>including martensite, ferrite, and damage sites, for subsequent analysis.<br>Either method shows substantial generalizability across a range of image sizes and conditions, including<br>heat-treated microstructures with different phase configurations. The nnU-Net excels in mapping large<br>image areas. Conversely, the GAN-based method performs reliably on smaller images, providing greater<br>step-by-step control and flexibility over the segmentation process.<br>This study highlights the benefits of segmented microstructural data for various purposes, such as<br>calculating phase fractions, modelling material behaviour through finite element simulation, and<br>conducting geometrical analyses of damage sites and the local properties of their surrounding<br>microstructure.</p> <p>https://doi.org/10.1016/j.matdes.2024.113031</p>
Fig. 3. Agarose gel image Fig. 4 in Optimization Of Dna Extraction Protocol For Dna Isolation From Air-Dried Collection Material For Further Phylogenetic Analysis (Coleoptera: Carabidae)
Fig. 3. Agarose gel image Fig. 4. Agarose gel image (successful PCR amplification) (failed PCR amplification) M: marker (bp) M: marker (bp) A1: Agonum fuliginosum Panzer, 1809 A: Agonum fuliginosum Panzer, 1809 A2: Agonum thoreyi Dejean, 1828 O: Omophron aequale aequale Morawitz, 1863 O: Omophron aequale aequale Morawitz, 1863 N: Notiophilus semistriatus Say, 1823 N: Notiophilus semistriatus Say, 1823 Nk: negative control. Nk: negative control.
Multi-modal image analysis for large scale cancer tissue studies within IMMUcan: multiplex immunofluorescence images
<p>In cancer research, multiplexed imaging has enabled the in-depth characterization of the tumor microenvironment (TME) and how it relates to patient prognosis. However, standardized, multi-modal data from large numbers of patients to identify robust biomarkers is missing. To provide such data across five cancer indications, the IMMUcan consortium performs broad molecular and cellular spatial profiling of thousands of cancer samples. Two reproducible and scalable workflows have been developed for whole slide multiplexed immunofluorescence (mIF) and imaging mass cytometry (IMC) to overcome challenges of reproducibility and scalability. For mIF we developed IFQuant, a web-based tool optimized for user-friendliness and reproducibility. This Zenodo record contains the mIF images and IFQuant settings to reproduce the results presented in the referenced publication. The companion IMC dataset is available as a joint Zenodo record.</p>
BRAIN Journal-Performance Analysis of Unsupervised Clustering Methods for Brain Tumor Segmentation-Figure 3:(a) Input MR Image (b) Enhanced Image (c) Segmented Tumor (d) Located brain tumor
<p>Figure 3 shows three different original brain MR images, contrast enhancement of the<br> images, segmented images using K-means algorithm and finally located tumor. Fig 1.4 shows the<br> performance of the unsupervised clustering methods with the no. of tumor pixels and execution<br> time to locate the brain tumor.</p>
Figure 9. Performance analysis of FCM-PSO, GPC-PSO and GFCM-PSO-An Optimized Clustering Approach for Automated Detection of White Matter Lesions in MRI Brain Images
<p>All scans obtained from different image clustering models are manually ranked based on<br> values in table 1. Table 2 represents WML detection rates of optimized images. FCM, GPC and<br> GFCM clustering methods and hybrid optimized methods (FCM-PSO, GPC-PSO and GFCM-PSO)<br> are applied on a dataset of 208 images and ranking is done in terms of under detected, over<br> detected, properly detected as shown in figure 8 and figure 9.</p>
Figure 8. Performance analysis of FCM, GPC and GFCM Figure 9.-An Optimized Clustering Approach for Automated Detection of White Matter Lesions in MRI Brain Images
<p>All scans obtained from different image clustering models are manually ranked based on<br> values in table 1. Table 2 represents WML detection rates of optimized images. FCM, GPC and<br> GFCM clustering methods and hybrid optimized methods (FCM-PSO, GPC-PSO and GFCM-PSO)<br> are applied on a dataset of 208 images and ranking is done in terms of under detected, over<br> detected, properly detected as shown in figure 8 and figure 9. The number of images detected<br> properly in GFCM is comparatively high than FCM and GPC. The optimized result of GFMC<br> provides accurate detection of WMLs and it properly detects 195 images.</p>
ENTICE VM image analysis and optimised fragmentation frequently built images dataset
<p>As part of the evaluation of ENTICE VM image analysis and optimised fragmentation services we have implemented a simulation environment which analyses online software package repositories (e.g. ones offered by the maintainers of the Ubuntu and Debian Linux distributions) and deduces decomposition options as well as expected fragment sizes based on metadata acquired from these repositories. This dataset contains the collected recipes for several frequently built Ubuntu Linux based VMIs (e.g., LAMP, LAPP, LEMP, LLMP, LYME, MEAN/MERN, LTM, etc.) and the calculated fragments and their relations. The dataset is used to analyse and evaluate the behaviour of the fragmentation services. The dataset is in compressed LRZIP format.</p>
Partitioned Image Data for Machine Learning Analysis of Molecular Biology Figures
<p><strong> Corpus Composition</strong></p> <p>This data collection provides four types of hand-curated images from open access research articles images. The types are:</p> <ol> <li>chart (n=811): data displays such as bar charts, scatterplots, line graphs, etc.</li> <li>diagram (n=816): any general conceptual diagram</li> <li>gel (n=1182): the output of electrophoresis experiments in Northern, Western, or Southern Blot experiments. </li> <li>histology (n=3458): microscope images of tissue with histological staining</li> </ol> <p>The images are simply organized in subdirectories as individual files. File names are based on PubMed Id and Figure number. </p>
Cross-modal (text and figures) Analysis of a Scientific Corpus from Semantic Scholar - images
<p>In this notebook we show the application of cross-modal techniques to improve the categorization of scientific papers through content related to figures, using both the textual part (captions) and the visual part (figures, diagrams, images) jointly. To this purpose, we use several CNN models and execute some experiments, illustrating our approach. This deposit contains high quality versions of the images used in the analysis.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.